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Measuring lymphocyte proliferation, survival and differentiation using CFSE time-series data.
Nat Protoc. 2007; 2(9):2057-67.NP

Abstract

Cellular proliferation is an essential feature of the adaptive immune response. The introduction of the division tracking dye carboxyfluorescein diacetate succinimidyl ester (CFSE) has made it possible to monitor the number of cell divisions during proliferation and to examine the relationship between proliferation and differentiation. Although qualitative examination of CFSE data may be useful, substantially more information about division and death rates can be extracted from quantitative CFSE time-series experiments. Quantitative methods can reveal in detail how lymphocyte proliferation and survival are regulated and altered by signals such as those received from co-stimulatory molecules, drugs and genetic polymorphisms. In this protocol, we present a detailed method for examining time-series data using graphical and computer-based procedures available to all experimenters.

Authors+Show Affiliations

Hawkins ED
Immunology Division, The Walter and Eliza Hall Institute of Medical Research, 1G Royal Parade, Parkville, Victoria, Australia.
Hommel M
No affiliation info available
Turner ML
No affiliation info available
Battye FL
No affiliation info available
Markham JF
No affiliation info available
Hodgkin PD
No affiliation info available

MeSH

Cell DifferentiationCell DivisionCell ProliferationCells, CulturedComputer SimulationData Interpretation, StatisticalFlow CytometryFluoresceinsFluorescent DyesLymphocyte ActivationLymphocytesModels, BiologicalSoftwareStaining and LabelingSuccinimides

Pub Type(s)

Journal Article

Language

eng

PubMed ID

17853861

Citation

Hawkins, Edwin D., et al. "Measuring Lymphocyte Proliferation, Survival and Differentiation Using CFSE Time-series Data." Nature Protocols, vol. 2, no. 9, 2007, pp. 2057-67.
Hawkins ED, Hommel M, Turner ML, et al. Measuring lymphocyte proliferation, survival and differentiation using CFSE time-series data. Nat Protoc. 2007;2(9):2057-67.
Hawkins, E. D., Hommel, M., Turner, M. L., Battye, F. L., Markham, J. F., & Hodgkin, P. D. (2007). Measuring lymphocyte proliferation, survival and differentiation using CFSE time-series data. Nature Protocols, 2(9), 2057-67.
Hawkins ED, et al. Measuring Lymphocyte Proliferation, Survival and Differentiation Using CFSE Time-series Data. Nat Protoc. 2007;2(9):2057-67. PubMed PMID: 17853861.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Measuring lymphocyte proliferation, survival and differentiation using CFSE time-series data. AU - Hawkins,Edwin D, AU - Hommel,Mirja, AU - Turner,Marian L, AU - Battye,Francis L, AU - Markham,John F, AU - Hodgkin,Philip D, PY - 2007/9/15/pubmed PY - 2007/12/7/medline PY - 2007/9/15/entrez SP - 2057 EP - 67 JF - Nature protocols JO - Nat Protoc VL - 2 IS - 9 N2 - Cellular proliferation is an essential feature of the adaptive immune response. The introduction of the division tracking dye carboxyfluorescein diacetate succinimidyl ester (CFSE) has made it possible to monitor the number of cell divisions during proliferation and to examine the relationship between proliferation and differentiation. Although qualitative examination of CFSE data may be useful, substantially more information about division and death rates can be extracted from quantitative CFSE time-series experiments. Quantitative methods can reveal in detail how lymphocyte proliferation and survival are regulated and altered by signals such as those received from co-stimulatory molecules, drugs and genetic polymorphisms. In this protocol, we present a detailed method for examining time-series data using graphical and computer-based procedures available to all experimenters. SN - 1750-2799 UR - https://www.unboundmedicine.com/medline/citation/17853861/Measuring_lymphocyte_proliferation_survival_and_differentiation_using_CFSE_time_series_data_ L2 - https://doi.org/10.1038/nprot.2007.297 DB - PRIME DP - Unbound Medicine ER -