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Analysis of transcripts and proteins expressed in the salivary glands of Hessian fly (Mayetiola destructor) larvae.
J Insect Physiol. 2008 Jan; 54(1):1-16.JI

Abstract

Hessian fly (Mayetiola destructor) larvae are thought to manipulate host growth and metabolism through salivary secretions. However, the transcriptome and proteome of Hessian fly salivary glands have not been systematically analyzed. In this research, we analyzed Expressed-Sequence-Tags (EST) representing 6106 cDNA clones randomly selected from four libraries made from dissected salivary glands. We also analyzed the protein composition of dissected salivary glands using one- and two-dimensional gel electrophoresis as well as LC-MS/MS analysis. Transcriptomic analysis revealed that approximately 60% of the total cDNA clones and 40% of assembled clusters encoded secretory proteins (SP). The SP-encoding cDNAs were grouped into superfamilies and families according to sequence similarities. In addition to the high percentage of SP-encoding transcripts, there was also a high percentage of transcripts encoding proteins that were either involved directly in protein synthesis or in house-keeping functions that provide conditions necessary for protein synthesis. Proteomic analysis also revealed a high percentage of proteins involved in protein synthesis either directly or indirectly. The high percentage of SP-encoding transcripts and high percentage of proteins related to protein synthesis suggested that the salivary glands of Hessian fly larvae are indeed specialized tissues for synthesis of proteins for host injection. However, LC-MS/MS analysis of 64 proteins did not identify any SPs corresponding to the cDNA sequences. The lack of accumulation of SPs in the salivary glands indicated the SPs were likely secreted as soon as they were synthesized.

Authors+Show Affiliations

USDA-ARS Plant Science and Entomology Research Unit, 4008 Throckmorton Hall, 1515 College Avenue, Manhattan, KS 66506, USA. mchen@ksu.eduNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Journal Article
Research Support, U.S. Gov't, Non-P.H.S.

Language

eng

PubMed ID

17854824

Citation

Chen, Ming-Shun, et al. "Analysis of Transcripts and Proteins Expressed in the Salivary Glands of Hessian Fly (Mayetiola Destructor) Larvae." Journal of Insect Physiology, vol. 54, no. 1, 2008, pp. 1-16.
Chen MS, Zhao HX, Zhu YC, et al. Analysis of transcripts and proteins expressed in the salivary glands of Hessian fly (Mayetiola destructor) larvae. J Insect Physiol. 2008;54(1):1-16.
Chen, M. S., Zhao, H. X., Zhu, Y. C., Scheffler, B., Liu, X., Liu, X., Hulbert, S., & Stuart, J. J. (2008). Analysis of transcripts and proteins expressed in the salivary glands of Hessian fly (Mayetiola destructor) larvae. Journal of Insect Physiology, 54(1), 1-16.
Chen MS, et al. Analysis of Transcripts and Proteins Expressed in the Salivary Glands of Hessian Fly (Mayetiola Destructor) Larvae. J Insect Physiol. 2008;54(1):1-16. PubMed PMID: 17854824.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Analysis of transcripts and proteins expressed in the salivary glands of Hessian fly (Mayetiola destructor) larvae. AU - Chen,Ming-Shun, AU - Zhao,Hui-Xian, AU - Zhu,Yu Cheng, AU - Scheffler,Brian, AU - Liu,Xuming, AU - Liu,Xiang, AU - Hulbert,Scot, AU - Stuart,Jeffrey J, Y1 - 2007/08/10/ PY - 2007/06/08/received PY - 2007/07/20/revised PY - 2007/07/24/accepted PY - 2007/9/15/pubmed PY - 2008/4/24/medline PY - 2007/9/15/entrez SP - 1 EP - 16 JF - Journal of insect physiology JO - J Insect Physiol VL - 54 IS - 1 N2 - Hessian fly (Mayetiola destructor) larvae are thought to manipulate host growth and metabolism through salivary secretions. However, the transcriptome and proteome of Hessian fly salivary glands have not been systematically analyzed. In this research, we analyzed Expressed-Sequence-Tags (EST) representing 6106 cDNA clones randomly selected from four libraries made from dissected salivary glands. We also analyzed the protein composition of dissected salivary glands using one- and two-dimensional gel electrophoresis as well as LC-MS/MS analysis. Transcriptomic analysis revealed that approximately 60% of the total cDNA clones and 40% of assembled clusters encoded secretory proteins (SP). The SP-encoding cDNAs were grouped into superfamilies and families according to sequence similarities. In addition to the high percentage of SP-encoding transcripts, there was also a high percentage of transcripts encoding proteins that were either involved directly in protein synthesis or in house-keeping functions that provide conditions necessary for protein synthesis. Proteomic analysis also revealed a high percentage of proteins involved in protein synthesis either directly or indirectly. The high percentage of SP-encoding transcripts and high percentage of proteins related to protein synthesis suggested that the salivary glands of Hessian fly larvae are indeed specialized tissues for synthesis of proteins for host injection. However, LC-MS/MS analysis of 64 proteins did not identify any SPs corresponding to the cDNA sequences. The lack of accumulation of SPs in the salivary glands indicated the SPs were likely secreted as soon as they were synthesized. SN - 0022-1910 UR - https://www.unboundmedicine.com/medline/citation/17854824/Analysis_of_transcripts_and_proteins_expressed_in_the_salivary_glands_of_Hessian_fly__Mayetiola_destructor__larvae_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0022-1910(07)00170-9 DB - PRIME DP - Unbound Medicine ER -