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Studies on the detection of antibody to duck hepatitis virus by enzyme-linked immunosorbent assay.
Avian Dis. 1991 Oct-Dec; 35(4):778-82.AD

Abstract

An indirect enzyme-linked immunosorbent assay (ELISA) for the detection of antibody to duck hepatitis virus (DHV) is described. The results of ELISA were compared with those of an agar gel diffusion precipitin (AGDP) test and a serum-neutralization (SN) test. The specificity of the ELISA was in accordance with the specificity of the AGDP and SN tests, but there was a difference in sensitivity. The positive detection rates of ELISA, SN test, and AGDP test for 93 clinical samples were 68.8%, 68.8%, and 18.8%, respectively. A positive/negative (P/N) value larger than or equal to 2.1 plus an absorbance value larger than or equal to 0.4 was used as a comprehensive positive standard for the ELISA. This eliminated false-positive reactions. The results showed that the ELISA was a rapid, sensitive, and accurate method for detecting antibody to DHV.

Authors+Show Affiliations

Shenzhen Animal and Plant Quarantine Service, Guangdong, People's Republic of China.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Journal Article

Language

eng

PubMed ID

1786011

Citation

Zhao, X L., et al. "Studies On the Detection of Antibody to Duck Hepatitis Virus By Enzyme-linked Immunosorbent Assay." Avian Diseases, vol. 35, no. 4, 1991, pp. 778-82.
Zhao XL, Phillips RM, Li GD, et al. Studies on the detection of antibody to duck hepatitis virus by enzyme-linked immunosorbent assay. Avian Dis. 1991;35(4):778-82.
Zhao, X. L., Phillips, R. M., Li, G. D., & Zhong, A. Q. (1991). Studies on the detection of antibody to duck hepatitis virus by enzyme-linked immunosorbent assay. Avian Diseases, 35(4), 778-82.
Zhao XL, et al. Studies On the Detection of Antibody to Duck Hepatitis Virus By Enzyme-linked Immunosorbent Assay. Avian Dis. 1991 Oct-Dec;35(4):778-82. PubMed PMID: 1786011.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Studies on the detection of antibody to duck hepatitis virus by enzyme-linked immunosorbent assay. AU - Zhao,X L, AU - Phillips,R M, AU - Li,G D, AU - Zhong,A Q, PY - 1991/10/1/pubmed PY - 1991/10/1/medline PY - 1991/10/1/entrez SP - 778 EP - 82 JF - Avian diseases JO - Avian Dis VL - 35 IS - 4 N2 - An indirect enzyme-linked immunosorbent assay (ELISA) for the detection of antibody to duck hepatitis virus (DHV) is described. The results of ELISA were compared with those of an agar gel diffusion precipitin (AGDP) test and a serum-neutralization (SN) test. The specificity of the ELISA was in accordance with the specificity of the AGDP and SN tests, but there was a difference in sensitivity. The positive detection rates of ELISA, SN test, and AGDP test for 93 clinical samples were 68.8%, 68.8%, and 18.8%, respectively. A positive/negative (P/N) value larger than or equal to 2.1 plus an absorbance value larger than or equal to 0.4 was used as a comprehensive positive standard for the ELISA. This eliminated false-positive reactions. The results showed that the ELISA was a rapid, sensitive, and accurate method for detecting antibody to DHV. SN - 0005-2086 UR - https://www.unboundmedicine.com/medline/citation/1786011/Studies_on_the_detection_of_antibody_to_duck_hepatitis_virus_by_enzyme_linked_immunosorbent_assay_ DB - PRIME DP - Unbound Medicine ER -