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Generation of an Frs2alpha conditional null allele.
Genesis 2007; 45(9):554-9G

Abstract

The fibroblast growth factor (FGF) signaling family controls a broad spectrum of cellular processes in development and adult tissue homeostasis and function, which is expressed in almost all tissues at all stages. FGF receptor substrate 2 alpha (FRS2alpha) is an adaptor protein that recruits downstream substrates to the FGF receptor (FGFR) tyrosine kinase. Disruption of Frs2alpha gene in mice abrogates activation of the mitogen-activated protein kinase pathway by the FGFR and leads to embryonic lethality at day E7.5 post copulation. To circumvent the embryonic lethality resulting from disruption of the Frs2alpha gene, which hinders further characterization of the role of FRS2alpha in adult tissue function and homeostasis, we generated an Frs2alpha conditional null allele for temporally- and tissue-specific disruption of the Frs2alpha gene. Using gene targeting in mouse embryonic stem cells, we introduced two loxP sites flanking the largest coding exon, exon 5, in the Frs2alpha allele. Our results indicate that the floxed Frs2alpha (Frs2alpha(flox)) allele is a true conditional null allele that encodes wildtype activity and is converted to a null allele after Cre recombinase mediated recombination.

Authors+Show Affiliations

Center for Cancer and Stem Cell Biology, Institute of Biosciences and Technology, Texas A&M Health Science Center, Houston, Texas 77030-3303, USA.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, Non-P.H.S.

Language

eng

PubMed ID

17868091

Citation

Lin, Yongshun, et al. "Generation of an Frs2alpha Conditional Null Allele." Genesis (New York, N.Y. : 2000), vol. 45, no. 9, 2007, pp. 554-9.
Lin Y, Zhang J, Zhang Y, et al. Generation of an Frs2alpha conditional null allele. Genesis. 2007;45(9):554-9.
Lin, Y., Zhang, J., Zhang, Y., & Wang, F. (2007). Generation of an Frs2alpha conditional null allele. Genesis (New York, N.Y. : 2000), 45(9), pp. 554-9.
Lin Y, et al. Generation of an Frs2alpha Conditional Null Allele. Genesis. 2007;45(9):554-9. PubMed PMID: 17868091.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Generation of an Frs2alpha conditional null allele. AU - Lin,Yongshun, AU - Zhang,Jue, AU - Zhang,Yongyou, AU - Wang,Fen, PY - 2007/9/18/pubmed PY - 2007/12/21/medline PY - 2007/9/18/entrez SP - 554 EP - 9 JF - Genesis (New York, N.Y. : 2000) JO - Genesis VL - 45 IS - 9 N2 - The fibroblast growth factor (FGF) signaling family controls a broad spectrum of cellular processes in development and adult tissue homeostasis and function, which is expressed in almost all tissues at all stages. FGF receptor substrate 2 alpha (FRS2alpha) is an adaptor protein that recruits downstream substrates to the FGF receptor (FGFR) tyrosine kinase. Disruption of Frs2alpha gene in mice abrogates activation of the mitogen-activated protein kinase pathway by the FGFR and leads to embryonic lethality at day E7.5 post copulation. To circumvent the embryonic lethality resulting from disruption of the Frs2alpha gene, which hinders further characterization of the role of FRS2alpha in adult tissue function and homeostasis, we generated an Frs2alpha conditional null allele for temporally- and tissue-specific disruption of the Frs2alpha gene. Using gene targeting in mouse embryonic stem cells, we introduced two loxP sites flanking the largest coding exon, exon 5, in the Frs2alpha allele. Our results indicate that the floxed Frs2alpha (Frs2alpha(flox)) allele is a true conditional null allele that encodes wildtype activity and is converted to a null allele after Cre recombinase mediated recombination. SN - 1526-954X UR - https://www.unboundmedicine.com/medline/citation/17868091/Generation_of_an_Frs2alpha_conditional_null_allele_ L2 - https://doi.org/10.1002/dvg.20327 DB - PRIME DP - Unbound Medicine ER -