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NMR characterization of the structure of a beta-(1-->3)-D-glucan isolate from cultured fruit bodies of Sparassis crispa.
Carbohydr Res. 2007 Dec 10; 342(17):2611-8.CR

Abstract

SCG, a purified beta-d-glucan, obtained from Sparassis crispa, exhibits various biological activities including an antitumor effect, enhancement of the hematopoietic response in cyclophosphamide-induced leukopenic mice, and induction of the production of cytokines. The mechanisms of these effects have been extensively investigated; however, an unambiguous structural characterization of SCG is yet to be achieved. It is well accepted that the biological effects of beta-glucan depend on its primary structures, conformation, and molecular weight. In the present study, we examine the difference of biological effects among beta-glucans, elucidate the primary structure of SCG, and compare with SPG from Schizophyllum commune using NMR spectroscopy. Our data reveal that SCG but not SPG induce cytokine production from bone marrow-derived dendritic cells (BMDCs) and their major structural units are a beta-(1-->3)-d-glucan backbone with single beta-(1-->6)-d-glucosyl side branching units every three residues.

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Authors+Show Affiliations

Tada R
Laboratory for Immunopharmacology of Microbial Products, School of Pharmacy, Tokyo University of Pharmacy and Life Science, 1432-1, Horinouchi, Hachioji, Tokyo 192-0392, Japan.
Harada T
No affiliation info available
Nagi-Miura N
No affiliation info available
Adachi Y
No affiliation info available
Nakajima M
No affiliation info available
Yadomae T
No affiliation info available
Ohno N
No affiliation info available

MeSH

AnimalsCarbohydrate SequenceFruitGlucansInterleukin-6Magnetic Resonance SpectroscopyMaleMiceMice, Inbred DBAMolecular ConformationMolecular Sequence DataPolysaccharidesPyransTumor Necrosis Factor-alphabeta-Glucans

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

17868661

Citation

Tada, Rui, et al. "NMR Characterization of the Structure of a beta-(1-->3)-D-glucan Isolate From Cultured Fruit Bodies of Sparassis Crispa." Carbohydrate Research, vol. 342, no. 17, 2007, pp. 2611-8.
Tada R, Harada T, Nagi-Miura N, et al. NMR characterization of the structure of a beta-(1-->3)-D-glucan isolate from cultured fruit bodies of Sparassis crispa. Carbohydr Res. 2007;342(17):2611-8.
Tada, R., Harada, T., Nagi-Miura, N., Adachi, Y., Nakajima, M., Yadomae, T., & Ohno, N. (2007). NMR characterization of the structure of a beta-(1-->3)-D-glucan isolate from cultured fruit bodies of Sparassis crispa. Carbohydrate Research, 342(17), 2611-8.
Tada R, et al. NMR Characterization of the Structure of a beta-(1-->3)-D-glucan Isolate From Cultured Fruit Bodies of Sparassis Crispa. Carbohydr Res. 2007 Dec 10;342(17):2611-8. PubMed PMID: 17868661.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - NMR characterization of the structure of a beta-(1-->3)-D-glucan isolate from cultured fruit bodies of Sparassis crispa. AU - Tada,Rui, AU - Harada,Toshie, AU - Nagi-Miura,Noriko, AU - Adachi,Yoshiyuki, AU - Nakajima,Mitsuhiro, AU - Yadomae,Toshiro, AU - Ohno,Naohito, Y1 - 2007/08/31/ PY - 2007/04/07/received PY - 2007/07/12/revised PY - 2007/08/17/accepted PY - 2007/9/18/pubmed PY - 2008/1/23/medline PY - 2007/9/18/entrez SP - 2611 EP - 8 JF - Carbohydrate research JO - Carbohydr Res VL - 342 IS - 17 N2 - SCG, a purified beta-d-glucan, obtained from Sparassis crispa, exhibits various biological activities including an antitumor effect, enhancement of the hematopoietic response in cyclophosphamide-induced leukopenic mice, and induction of the production of cytokines. The mechanisms of these effects have been extensively investigated; however, an unambiguous structural characterization of SCG is yet to be achieved. It is well accepted that the biological effects of beta-glucan depend on its primary structures, conformation, and molecular weight. In the present study, we examine the difference of biological effects among beta-glucans, elucidate the primary structure of SCG, and compare with SPG from Schizophyllum commune using NMR spectroscopy. Our data reveal that SCG but not SPG induce cytokine production from bone marrow-derived dendritic cells (BMDCs) and their major structural units are a beta-(1-->3)-d-glucan backbone with single beta-(1-->6)-d-glucosyl side branching units every three residues. SN - 0008-6215 UR - https://www.unboundmedicine.com/medline/citation/17868661/NMR_characterization_of_the_structure_of_a_beta__1__>3__D_glucan_isolate_from_cultured_fruit_bodies_of_Sparassis_crispa_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0008-6215(07)00370-9 DB - PRIME DP - Unbound Medicine ER -