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Generation of an attenuated H5N1 avian influenza virus vaccine with all eight genes from avian viruses.
Vaccine. 2007 Oct 16; 25(42):7379-84.V

Abstract

In the face of disease outbreaks in poultry and the potential pandemic threat to humans caused by the highly pathogenic avian influenza viruses (HPAIVs) of H5N1 subtype, improvement in biosecurity and the use of inactivated vaccines are two main options for the control of this disease. Vaccine candidates of influenza A viruses of H5N1 subtype have been generated in several laboratories by plasmid-based reverse genetics with hemagglutinin (HA) and neuraminidase (NA) genes from the epidemic strains of avian viruses in a background of internal genes from the vaccine donor strain of human strains, A/Puerto Rico/8/34 (PR8). These reassortant viruses containing genes from both avian and human viruses might impose biosafety concerns, also may be do if C4/F AIV would be a live attenuated vaccine or cold-adaptive strain vaccine. In order to generate better and safer vaccine candidate viruses, we genetically constructed attenuated reassortant H5N1 influenza A virus, designated as C4/F AIV, by plasmid-based reverse genetics with all eight genes from the avian strains. The C4/F AIV virus contained HA and NA genes from an epidemic strain A/Chicken/Huadong/04 (H5N1) (C4/H5N1) in a background of internal genes derived from a low pathogenic strain of A/Chicken/F/98(H9N2). The reassortant virus was attenuated by removal of the multibasic amino acid motif in the HA gene by mutation and deletion (from PQRERRRKKR (downward arrow) G to PQIETR (downward arrow) G). The intravenous pathogenicity index (IVPI) of C4/F AIV virus was 0, whereas that of the donor virus C4/H5N1 was 3.0. The virus HA titer of C4/H5N1 in the allantoic fluid from infected embryonated eggs was as high as 1:2048. The inactivated vaccine prepared from the reassortant virus C4/F AIV-induced high HI titer in vaccinated chickens and gave 100% protection when challenged with highly pathogenic avian influenza virus of H5N1 subtype.

Authors+Show Affiliations

Shi H
Animal Infectious Disease Laboratory, School of Veterinary Medicine, Yangzhou University, 225009 Yangzhou, Jiangsu, People's Republic of China. hyshi@yzu.edu.cn
Liu XF
No affiliation info available
Zhang X
No affiliation info available
Chen S
No affiliation info available
Sun L
No affiliation info available
Lu J
No affiliation info available

MeSH

Amino Acid SequenceAnimalsBase SequenceChickensDNA, ViralFemaleFormaldehydeGenes, ViralHemagglutinin Glycoproteins, Influenza VirusHumansInfluenza A Virus, H5N1 SubtypeInfluenza VaccinesInfluenza in BirdsInfluenza, HumanMiceMice, Inbred BALB CVaccines, AttenuatedVaccines, InactivatedVirus Replication

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

17870216

Citation

Shi, Huoying, et al. "Generation of an Attenuated H5N1 Avian Influenza Virus Vaccine With All Eight Genes From Avian Viruses." Vaccine, vol. 25, no. 42, 2007, pp. 7379-84.
Shi H, Liu XF, Zhang X, et al. Generation of an attenuated H5N1 avian influenza virus vaccine with all eight genes from avian viruses. Vaccine. 2007;25(42):7379-84.
Shi, H., Liu, X. F., Zhang, X., Chen, S., Sun, L., & Lu, J. (2007). Generation of an attenuated H5N1 avian influenza virus vaccine with all eight genes from avian viruses. Vaccine, 25(42), 7379-84.
Shi H, et al. Generation of an Attenuated H5N1 Avian Influenza Virus Vaccine With All Eight Genes From Avian Viruses. Vaccine. 2007 Oct 16;25(42):7379-84. PubMed PMID: 17870216.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Generation of an attenuated H5N1 avian influenza virus vaccine with all eight genes from avian viruses. AU - Shi,Huoying, AU - Liu,Xiu Fan, AU - Zhang,Xiaorong, AU - Chen,Sujuan, AU - Sun,Lei, AU - Lu,Jianhong, Y1 - 2007/08/24/ PY - 2007/07/29/received PY - 2007/08/07/accepted PY - 2007/9/18/pubmed PY - 2007/12/20/medline PY - 2007/9/18/entrez SP - 7379 EP - 84 JF - Vaccine JO - Vaccine VL - 25 IS - 42 N2 - In the face of disease outbreaks in poultry and the potential pandemic threat to humans caused by the highly pathogenic avian influenza viruses (HPAIVs) of H5N1 subtype, improvement in biosecurity and the use of inactivated vaccines are two main options for the control of this disease. Vaccine candidates of influenza A viruses of H5N1 subtype have been generated in several laboratories by plasmid-based reverse genetics with hemagglutinin (HA) and neuraminidase (NA) genes from the epidemic strains of avian viruses in a background of internal genes from the vaccine donor strain of human strains, A/Puerto Rico/8/34 (PR8). These reassortant viruses containing genes from both avian and human viruses might impose biosafety concerns, also may be do if C4/F AIV would be a live attenuated vaccine or cold-adaptive strain vaccine. In order to generate better and safer vaccine candidate viruses, we genetically constructed attenuated reassortant H5N1 influenza A virus, designated as C4/F AIV, by plasmid-based reverse genetics with all eight genes from the avian strains. The C4/F AIV virus contained HA and NA genes from an epidemic strain A/Chicken/Huadong/04 (H5N1) (C4/H5N1) in a background of internal genes derived from a low pathogenic strain of A/Chicken/F/98(H9N2). The reassortant virus was attenuated by removal of the multibasic amino acid motif in the HA gene by mutation and deletion (from PQRERRRKKR (downward arrow) G to PQIETR (downward arrow) G). The intravenous pathogenicity index (IVPI) of C4/F AIV virus was 0, whereas that of the donor virus C4/H5N1 was 3.0. The virus HA titer of C4/H5N1 in the allantoic fluid from infected embryonated eggs was as high as 1:2048. The inactivated vaccine prepared from the reassortant virus C4/F AIV-induced high HI titer in vaccinated chickens and gave 100% protection when challenged with highly pathogenic avian influenza virus of H5N1 subtype. SN - 0264-410X UR - https://www.unboundmedicine.com/medline/citation/17870216/Generation_of_an_attenuated_H5N1_avian_influenza_virus_vaccine_with_all_eight_genes_from_avian_viruses_ DB - PRIME DP - Unbound Medicine ER -