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Validated stability-indicating densitometric thin-layer chromatography: application to stress degradation studies of minocycline.
Anal Chim Acta 2007; 599(2):302-9AC

Abstract

A simple, stability-indicating high-performance thin-layer liquid chromatographic (HPTLC) method for analysis of minocycline was developed and validated. The densitometric analysis was carried out at 345 nm using methanol-acetonitrile-isopropyl alcohol-water (5:4:0.5:0.5, v/v/v/v) as mobile phase. The method employed TLC aluminium plates pre-coated with silica gel 60F-254 as the stationary phase. To achieve good result, plates were sprayed with a 10% (w/v) solution of disodium ethylene diaminetetraacetic acid (EDTA), the pH of which was adjusted to 9.0. Compact spots of minocycline were found at R(f) = 0.30+/-0.02. For proposed procedure, linearity (r = 0.9997), limit of detection (3.7 ng spot(-1)), recovery (99.23-100.16%), and precision (% R.S.D. < or = 0.364) was found to be satisfactory. The drug undergoes acidic and basic degradation, oxidation and photodegradation. All the peaks of degradation products were well resolved from the pure drug with significantly different R(f) values. The acidic and alkaline degradation kinetics of minocycline, evaluated using this method, is found to be of first order.

Authors+Show Affiliations

Department of Pharmaceutics, Faculty of Pharmacy, Hamdard University, Hamdard Nagar, New Delhi 110062, India. jneelu78@yahoo.comNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Validation Studies

Language

eng

PubMed ID

17870294

Citation

Jain, Nilu, et al. "Validated Stability-indicating Densitometric Thin-layer Chromatography: Application to Stress Degradation Studies of Minocycline." Analytica Chimica Acta, vol. 599, no. 2, 2007, pp. 302-9.
Jain N, Jain GK, Ahmad FJ, et al. Validated stability-indicating densitometric thin-layer chromatography: application to stress degradation studies of minocycline. Anal Chim Acta. 2007;599(2):302-9.
Jain, N., Jain, G. K., Ahmad, F. J., & Khar, R. K. (2007). Validated stability-indicating densitometric thin-layer chromatography: application to stress degradation studies of minocycline. Analytica Chimica Acta, 599(2), pp. 302-9.
Jain N, et al. Validated Stability-indicating Densitometric Thin-layer Chromatography: Application to Stress Degradation Studies of Minocycline. Anal Chim Acta. 2007 Sep 19;599(2):302-9. PubMed PMID: 17870294.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Validated stability-indicating densitometric thin-layer chromatography: application to stress degradation studies of minocycline. AU - Jain,Nilu, AU - Jain,Gaurav Kumar, AU - Ahmad,Farhan Jalees, AU - Khar,Roop Krishen, Y1 - 2007/08/19/ PY - 2007/05/22/received PY - 2007/08/06/revised PY - 2007/08/09/accepted PY - 2007/9/18/pubmed PY - 2007/10/6/medline PY - 2007/9/18/entrez SP - 302 EP - 9 JF - Analytica chimica acta JO - Anal. Chim. Acta VL - 599 IS - 2 N2 - A simple, stability-indicating high-performance thin-layer liquid chromatographic (HPTLC) method for analysis of minocycline was developed and validated. The densitometric analysis was carried out at 345 nm using methanol-acetonitrile-isopropyl alcohol-water (5:4:0.5:0.5, v/v/v/v) as mobile phase. The method employed TLC aluminium plates pre-coated with silica gel 60F-254 as the stationary phase. To achieve good result, plates were sprayed with a 10% (w/v) solution of disodium ethylene diaminetetraacetic acid (EDTA), the pH of which was adjusted to 9.0. Compact spots of minocycline were found at R(f) = 0.30+/-0.02. For proposed procedure, linearity (r = 0.9997), limit of detection (3.7 ng spot(-1)), recovery (99.23-100.16%), and precision (% R.S.D. < or = 0.364) was found to be satisfactory. The drug undergoes acidic and basic degradation, oxidation and photodegradation. All the peaks of degradation products were well resolved from the pure drug with significantly different R(f) values. The acidic and alkaline degradation kinetics of minocycline, evaluated using this method, is found to be of first order. SN - 1873-4324 UR - https://www.unboundmedicine.com/medline/citation/17870294/Validated_stability_indicating_densitometric_thin_layer_chromatography:_application_to_stress_degradation_studies_of_minocycline_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0003-2670(07)01346-3 DB - PRIME DP - Unbound Medicine ER -