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Iron-chelating agents never suppress Fenton reaction but participate in quenching spin-trapped radicals.
Anal Chim Acta. 2007 Sep 19; 599(2):315-9.AC

Abstract

Hydroxyl radical formation by Fenton reaction in the presence of an iron-chelating agent such as EDTA was traced by two different assay methods; an electron spin resonance (ESR) spin-trapping method with 5,5-dimethyl-1-pyrroline N-oxide (DMPO), and high Performance liquid chromatography (HPLC)-fluorescence detection with terephthalic acid (TPA), a fluorescent probe for hydroxyl radicals. From the ESR spin-trapping measurement, it was observed that EDTA seemed to suppress hydroxyl radical formation with the increase of its concentration. On the other hand, hydroxyl radical formation by Fenton reaction was not affected by EDTA monitored by HPLC assay. Similar inconsistent effects of other iron-chelating agents such as nitrylotriacetic acid (NTA), diethylenetriamine penta acetic acid (DTPA), oxalate and citrate were also observed. On the addition of EDTA solution to the reaction mixture 10 min after the Fenton reaction started, when hydroxyl radical formation should have almost ceased but the ESR signal of DMPO-OH radicals could be detected, it was observed that the DMPO-OH* signal disappeared rapidly. With the simultaneous addition of Fe(II) solution and EDTA after the Fenton reaction ceased, the DMPO-OH* signal disappeared more rapidly. The results indicated that these chelating agents should enhance the quenching of [DMPO-OH]* radicals by Fe(II), but they did not suppress Fenton reaction by forming chelates with iron ions.

Authors+Show Affiliations

Kyoritsu University of Pharmacy, 1-5-30 Shibakoen, Minato, Tokyo 105-8512, Japan.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

17870296

Citation

Li, Linxiang, et al. "Iron-chelating Agents Never Suppress Fenton Reaction but Participate in Quenching Spin-trapped Radicals." Analytica Chimica Acta, vol. 599, no. 2, 2007, pp. 315-9.
Li L, Abe Y, Kanagawa K, et al. Iron-chelating agents never suppress Fenton reaction but participate in quenching spin-trapped radicals. Anal Chim Acta. 2007;599(2):315-9.
Li, L., Abe, Y., Kanagawa, K., Shoji, T., Mashino, T., Mochizuki, M., Tanaka, M., & Miyata, N. (2007). Iron-chelating agents never suppress Fenton reaction but participate in quenching spin-trapped radicals. Analytica Chimica Acta, 599(2), 315-9.
Li L, et al. Iron-chelating Agents Never Suppress Fenton Reaction but Participate in Quenching Spin-trapped Radicals. Anal Chim Acta. 2007 Sep 19;599(2):315-9. PubMed PMID: 17870296.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Iron-chelating agents never suppress Fenton reaction but participate in quenching spin-trapped radicals. AU - Li,Linxiang, AU - Abe,Yoshihiro, AU - Kanagawa,Kiyotada, AU - Shoji,Tomoko, AU - Mashino,Tadahiko, AU - Mochizuki,Masataka, AU - Tanaka,Miho, AU - Miyata,Naoki, Y1 - 2007/08/06/ PY - 2007/06/04/received PY - 2007/08/01/revised PY - 2007/08/03/accepted PY - 2007/9/18/pubmed PY - 2007/10/6/medline PY - 2007/9/18/entrez SP - 315 EP - 9 JF - Analytica chimica acta JO - Anal. Chim. Acta VL - 599 IS - 2 N2 - Hydroxyl radical formation by Fenton reaction in the presence of an iron-chelating agent such as EDTA was traced by two different assay methods; an electron spin resonance (ESR) spin-trapping method with 5,5-dimethyl-1-pyrroline N-oxide (DMPO), and high Performance liquid chromatography (HPLC)-fluorescence detection with terephthalic acid (TPA), a fluorescent probe for hydroxyl radicals. From the ESR spin-trapping measurement, it was observed that EDTA seemed to suppress hydroxyl radical formation with the increase of its concentration. On the other hand, hydroxyl radical formation by Fenton reaction was not affected by EDTA monitored by HPLC assay. Similar inconsistent effects of other iron-chelating agents such as nitrylotriacetic acid (NTA), diethylenetriamine penta acetic acid (DTPA), oxalate and citrate were also observed. On the addition of EDTA solution to the reaction mixture 10 min after the Fenton reaction started, when hydroxyl radical formation should have almost ceased but the ESR signal of DMPO-OH radicals could be detected, it was observed that the DMPO-OH* signal disappeared rapidly. With the simultaneous addition of Fe(II) solution and EDTA after the Fenton reaction ceased, the DMPO-OH* signal disappeared more rapidly. The results indicated that these chelating agents should enhance the quenching of [DMPO-OH]* radicals by Fe(II), but they did not suppress Fenton reaction by forming chelates with iron ions. SN - 1873-4324 UR - https://www.unboundmedicine.com/medline/citation/17870296/Iron_chelating_agents_never_suppress_Fenton_reaction_but_participate_in_quenching_spin_trapped_radicals_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0003-2670(07)01339-6 DB - PRIME DP - Unbound Medicine ER -