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Expression of the vascular endothelial growth factor-receptor system in the porcine endometrium throughout the estrous cycle and early pregnancy.
Mol Reprod Dev. 2008 Feb; 75(2):362-72.MR

Abstract

The objective of this study was to investigate the protein and mRNA expression of vascular endothelial growth factor (VEGF), VEGFR-1 (fms-like tyrosine kinase, Flt-1) and VEGFR-2 (fetal liver kinase-1/kinase insert domain-containing receptor, Flk-1/KDR) in the endometrium during the estrous cycle and early pregnancy in pigs. The VEGF-receptor system was localized in epithelial and stromal cells, blood vessels, and myometrium. Western blot analysis showed higher levels of VEGF protein during the periovulatory and periimplantation periods (P < 0.001, and P < 0.05, respectively). Constant expression of VEGF mRNA during the cycle and significant upregulation on Days 22-25 of gestation (vs. Days 9-17; P < 0.001) was observed. Stable levels of VEGFR-1 mRNA and protein were detected in the endometrium of cyclic animals. However, higher VEGFR-1 protein expression was found on Days 16-17 of the estrous cycle (P < 0.01) and Days 13-15 of gestation (P < 0.05). Protein expression of VEGFR-2 was elevated on Days 2-4 of the estrous cycle (P < 0.001), but mRNA levels were constant during the cycle. In pregnancy, VEGFR-2 protein expression started to increase after Day 15 (vs. Days 9-12; P < 0.05), but induction of VEGFR-2 mRNA expression occurred earlier on Days 13-15. It appears from the present study that the VEGF-receptor system is regulated in a temporal and spatial manner during the estrous cycle and early pregnancy in pigs. The results suggest that VEGF-A family members are probably involved in appropriate preparation of endometrium for implantation and in vascular events during implantation in pigs.

Authors+Show Affiliations

Division of Reproductive Endocrinology and Pathophysiology, Institute of Animal Reproduction and Food Research, Polish Academy of Sciences, Olsztyn, Poland. moka@pan.olsztyn.plNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

17874446

Citation

Kaczmarek, Monika M., et al. "Expression of the Vascular Endothelial Growth Factor-receptor System in the Porcine Endometrium Throughout the Estrous Cycle and Early Pregnancy." Molecular Reproduction and Development, vol. 75, no. 2, 2008, pp. 362-72.
Kaczmarek MM, Waclawik A, Blitek A, et al. Expression of the vascular endothelial growth factor-receptor system in the porcine endometrium throughout the estrous cycle and early pregnancy. Mol Reprod Dev. 2008;75(2):362-72.
Kaczmarek, M. M., Waclawik, A., Blitek, A., Kowalczyk, A. E., Schams, D., & Ziecik, A. J. (2008). Expression of the vascular endothelial growth factor-receptor system in the porcine endometrium throughout the estrous cycle and early pregnancy. Molecular Reproduction and Development, 75(2), 362-72.
Kaczmarek MM, et al. Expression of the Vascular Endothelial Growth Factor-receptor System in the Porcine Endometrium Throughout the Estrous Cycle and Early Pregnancy. Mol Reprod Dev. 2008;75(2):362-72. PubMed PMID: 17874446.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Expression of the vascular endothelial growth factor-receptor system in the porcine endometrium throughout the estrous cycle and early pregnancy. AU - Kaczmarek,Monika M, AU - Waclawik,Agnieszka, AU - Blitek,Agnieszka, AU - Kowalczyk,Anna E, AU - Schams,Dieter, AU - Ziecik,Adam J, PY - 2007/9/18/pubmed PY - 2008/5/6/medline PY - 2007/9/18/entrez SP - 362 EP - 72 JF - Molecular reproduction and development JO - Mol Reprod Dev VL - 75 IS - 2 N2 - The objective of this study was to investigate the protein and mRNA expression of vascular endothelial growth factor (VEGF), VEGFR-1 (fms-like tyrosine kinase, Flt-1) and VEGFR-2 (fetal liver kinase-1/kinase insert domain-containing receptor, Flk-1/KDR) in the endometrium during the estrous cycle and early pregnancy in pigs. The VEGF-receptor system was localized in epithelial and stromal cells, blood vessels, and myometrium. Western blot analysis showed higher levels of VEGF protein during the periovulatory and periimplantation periods (P < 0.001, and P < 0.05, respectively). Constant expression of VEGF mRNA during the cycle and significant upregulation on Days 22-25 of gestation (vs. Days 9-17; P < 0.001) was observed. Stable levels of VEGFR-1 mRNA and protein were detected in the endometrium of cyclic animals. However, higher VEGFR-1 protein expression was found on Days 16-17 of the estrous cycle (P < 0.01) and Days 13-15 of gestation (P < 0.05). Protein expression of VEGFR-2 was elevated on Days 2-4 of the estrous cycle (P < 0.001), but mRNA levels were constant during the cycle. In pregnancy, VEGFR-2 protein expression started to increase after Day 15 (vs. Days 9-12; P < 0.05), but induction of VEGFR-2 mRNA expression occurred earlier on Days 13-15. It appears from the present study that the VEGF-receptor system is regulated in a temporal and spatial manner during the estrous cycle and early pregnancy in pigs. The results suggest that VEGF-A family members are probably involved in appropriate preparation of endometrium for implantation and in vascular events during implantation in pigs. SN - 1040-452X UR - https://www.unboundmedicine.com/medline/citation/17874446/Expression_of_the_vascular_endothelial_growth_factor_receptor_system_in_the_porcine_endometrium_throughout_the_estrous_cycle_and_early_pregnancy_ L2 - https://doi.org/10.1002/mrd.20815 DB - PRIME DP - Unbound Medicine ER -