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Transient receptor potential channels in mechanosensing and cell volume regulation.
Methods Enzymol. 2007; 428:183-207.ME

Abstract

Transient receptor potential (TRP) channels are unique cellular sensors responding to a wide variety of extra- and intracellular signals, including mechanical and osmotic stress. In recent years, TRP channels from multiple subfamilies have been added to the list of mechano- and/or osmosensitive channels, and it is becoming increasingly apparent that Ca(2+) influx via TRP channels plays a crucial role in the response to mechanical and osmotic perturbations in a wide range of cell types. Although the events translating mechanical and osmotic stimuli into regulation of TRP channels are still incompletely understood, the specific mechanisms employed vary between different TRP isoforms, and probably include changes in the tension and/or curvature of the lipid bilayer, changes in the cortical cytoskeleton, and signaling events such as lipid metabolism and protein phosphorylation/dephosphorylation. This chapter describes candidate mechanosensitive channels from mammalian TRP subfamilies, discusses inherent and technical issues potentially confounding evaluation of mechano- and/or osmosensitivity, and presents methods relevant to the study of TRP channel regulation by mechanical and osmotic stimuli and involvement in cell volume regulation.

Authors+Show Affiliations

Department of Molecular Biology, University of Copenhagen, Copenhagen, Denmark.No affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

17875418

Citation

Pedersen, Stine Falsig, and Bernd Nilius. "Transient Receptor Potential Channels in Mechanosensing and Cell Volume Regulation." Methods in Enzymology, vol. 428, 2007, pp. 183-207.
Pedersen SF, Nilius B. Transient receptor potential channels in mechanosensing and cell volume regulation. Methods Enzymol. 2007;428:183-207.
Pedersen, S. F., & Nilius, B. (2007). Transient receptor potential channels in mechanosensing and cell volume regulation. Methods in Enzymology, 428, 183-207.
Pedersen SF, Nilius B. Transient Receptor Potential Channels in Mechanosensing and Cell Volume Regulation. Methods Enzymol. 2007;428:183-207. PubMed PMID: 17875418.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Transient receptor potential channels in mechanosensing and cell volume regulation. AU - Pedersen,Stine Falsig, AU - Nilius,Bernd, PY - 2007/9/19/pubmed PY - 2007/11/6/medline PY - 2007/9/19/entrez SP - 183 EP - 207 JF - Methods in enzymology JO - Methods Enzymol VL - 428 N2 - Transient receptor potential (TRP) channels are unique cellular sensors responding to a wide variety of extra- and intracellular signals, including mechanical and osmotic stress. In recent years, TRP channels from multiple subfamilies have been added to the list of mechano- and/or osmosensitive channels, and it is becoming increasingly apparent that Ca(2+) influx via TRP channels plays a crucial role in the response to mechanical and osmotic perturbations in a wide range of cell types. Although the events translating mechanical and osmotic stimuli into regulation of TRP channels are still incompletely understood, the specific mechanisms employed vary between different TRP isoforms, and probably include changes in the tension and/or curvature of the lipid bilayer, changes in the cortical cytoskeleton, and signaling events such as lipid metabolism and protein phosphorylation/dephosphorylation. This chapter describes candidate mechanosensitive channels from mammalian TRP subfamilies, discusses inherent and technical issues potentially confounding evaluation of mechano- and/or osmosensitivity, and presents methods relevant to the study of TRP channel regulation by mechanical and osmotic stimuli and involvement in cell volume regulation. SN - 0076-6879 UR - https://www.unboundmedicine.com/medline/citation/17875418/Transient_receptor_potential_channels_in_mechanosensing_and_cell_volume_regulation_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0076-6879(07)28010-3 DB - PRIME DP - Unbound Medicine ER -