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Bronchial mucosal dendritic cells in smokers and ex-smokers with COPD: an electron microscopic study.
Thorax. 2008 Feb; 63(2):108-14.T

Abstract

BACKGROUND

Bronchial mucosal dendritic cells (DCs) initiate and regulate immune responses to inhaled antigens, viruses and bacteria. Currently, little is known of their numbers in patients with chronic obstructive pulmonary disease (COPD). While reductions in their numbers have been reported recently in smokers with asthma, nothing is known of the effects of cigarette smoking on bronchial DCs in COPD. The present study compares DC numbers in smokers and ex-smokers with COPD.

METHODS

Endobronchial biopsies were obtained from 15 patients with moderate to severe COPD (10 current smokers with median forced expiratory volume in 1 s (FEV1) 45.5% predicted (range 23-68) and 5 ex-smokers with median FEV1 30% predicted (range 21-52)), 11 non-smokers with asthma (median FEV1 102% predicted (range 89-116)) and 11 non-smoker healthy controls (median FEV1 110% predicted (range 92-135)). Transmission electron microscopy (TEM) was used to identify the total population of DCs by their ultrastructure and their number in the epithelium and subepithelium was counted.

RESULTS

Median (range) DC numbers were significantly lower in current smokers with COPD in the epithelium (0.0 (0.0-156.8) cells/mm2) and the subepithelium (4.5 (0.0-63.6) cells/mm2) compared with ex-smokers with COPD (97.9 (93.5-170.3) cells/mm2 in the epithelium (p<0.05); 91.8 (38.2-283.3) cells/mm2 in the subepithelium (p<0.01)). DC numbers in ex-smokers with COPD were similar to those in subjects with atopic asthma and healthy controls (131.6 (33.3-235.5) cells/mm2 in the epithelium and 64.4 (0.0-182.4) cells/mm2 in the subepithelium for the latter).

CONCLUSIONS

In COPD, bronchial mucosal DC numbers are lower in current smokers while, in those who quit, numbers are similar to non-smoking subjects with asthma and non-smoking healthy controls. The functional consequences of the reduction in mucosal DC numbers in smokers with COPD have yet to be determined.

Authors+Show Affiliations

Lung Pathology Unit, Department of Gene Therapy, Imperial College London, London, UK.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Multicenter Study
Research Support, Non-U.S. Gov't
Validation Study

Language

eng

PubMed ID

17875567

Citation

Rogers, A V., et al. "Bronchial Mucosal Dendritic Cells in Smokers and Ex-smokers With COPD: an Electron Microscopic Study." Thorax, vol. 63, no. 2, 2008, pp. 108-14.
Rogers AV, Adelroth E, Hattotuwa K, et al. Bronchial mucosal dendritic cells in smokers and ex-smokers with COPD: an electron microscopic study. Thorax. 2008;63(2):108-14.
Rogers, A. V., Adelroth, E., Hattotuwa, K., Dewar, A., & Jeffery, P. K. (2008). Bronchial mucosal dendritic cells in smokers and ex-smokers with COPD: an electron microscopic study. Thorax, 63(2), 108-14.
Rogers AV, et al. Bronchial Mucosal Dendritic Cells in Smokers and Ex-smokers With COPD: an Electron Microscopic Study. Thorax. 2008;63(2):108-14. PubMed PMID: 17875567.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Bronchial mucosal dendritic cells in smokers and ex-smokers with COPD: an electron microscopic study. AU - Rogers,A V, AU - Adelroth,E, AU - Hattotuwa,K, AU - Dewar,A, AU - Jeffery,P K, Y1 - 2007/09/17/ PY - 2007/9/19/pubmed PY - 2008/2/15/medline PY - 2007/9/19/entrez SP - 108 EP - 14 JF - Thorax JO - Thorax VL - 63 IS - 2 N2 - BACKGROUND: Bronchial mucosal dendritic cells (DCs) initiate and regulate immune responses to inhaled antigens, viruses and bacteria. Currently, little is known of their numbers in patients with chronic obstructive pulmonary disease (COPD). While reductions in their numbers have been reported recently in smokers with asthma, nothing is known of the effects of cigarette smoking on bronchial DCs in COPD. The present study compares DC numbers in smokers and ex-smokers with COPD. METHODS: Endobronchial biopsies were obtained from 15 patients with moderate to severe COPD (10 current smokers with median forced expiratory volume in 1 s (FEV1) 45.5% predicted (range 23-68) and 5 ex-smokers with median FEV1 30% predicted (range 21-52)), 11 non-smokers with asthma (median FEV1 102% predicted (range 89-116)) and 11 non-smoker healthy controls (median FEV1 110% predicted (range 92-135)). Transmission electron microscopy (TEM) was used to identify the total population of DCs by their ultrastructure and their number in the epithelium and subepithelium was counted. RESULTS: Median (range) DC numbers were significantly lower in current smokers with COPD in the epithelium (0.0 (0.0-156.8) cells/mm2) and the subepithelium (4.5 (0.0-63.6) cells/mm2) compared with ex-smokers with COPD (97.9 (93.5-170.3) cells/mm2 in the epithelium (p<0.05); 91.8 (38.2-283.3) cells/mm2 in the subepithelium (p<0.01)). DC numbers in ex-smokers with COPD were similar to those in subjects with atopic asthma and healthy controls (131.6 (33.3-235.5) cells/mm2 in the epithelium and 64.4 (0.0-182.4) cells/mm2 in the subepithelium for the latter). CONCLUSIONS: In COPD, bronchial mucosal DC numbers are lower in current smokers while, in those who quit, numbers are similar to non-smoking subjects with asthma and non-smoking healthy controls. The functional consequences of the reduction in mucosal DC numbers in smokers with COPD have yet to be determined. SN - 1468-3296 UR - https://www.unboundmedicine.com/medline/citation/17875567/Bronchial_mucosal_dendritic_cells_in_smokers_and_ex_smokers_with_COPD:_an_electron_microscopic_study_ L2 - https://thorax.bmj.com/lookup/pmidlookup?view=long&amp;pmid=17875567 DB - PRIME DP - Unbound Medicine ER -