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Control of glycinergic input to spinal dorsal horn neurons by distinct muscarinic receptor subtypes revealed using knockout mice.
J Pharmacol Exp Ther. 2007 Dec; 323(3):963-71.JP

Abstract

Muscarinic acetylcholine receptors (mAChRs) play an important role in the tonic regulation of nociceptive transmission in the spinal cord. However, how mAChR subtypes contribute to the regulation of synaptic glycine release is unknown. To determine their role, glycinergic spontaneous inhibitory postsynaptic currents (sIPSCs) were recorded in lamina II neurons by using whole-cell recordings in spinal cord slices of wild-type (WT) and mAChR subtype knockout (KO) mice. In WT mice, the mAChR agonist oxotremorine-M dose-dependently decreased the frequency of sIPSCs in most neurons, but it had variable effects in other neurons. In contrast, in M3-KO mice, oxotremorine-M consistently decreased the glycinergic sIPSC frequency in all neurons tested, and in M2/M4 double-KO mice, it always increased the sIPSC frequency. In M2/M4 double-KO mice, the potentiating effect of oxotremorine-M was attenuated by higher concentrations in some neurons through activation of GABA(B) receptors. In pertussis toxin-treated WT mice, oxotremorine-M also consistently increased the sIPSC frequency. In M2-KO and M4-KO mice, the effect of oxotremorine-M on sIPSCs was divergent because of the opposing functions of the M3 subtype and the M2 and M4 subtypes. This study demonstrates that stimulation of the M2 and M4 subtypes inhibits glycinergic inputs to spinal dorsal horn neurons of mice, whereas stimulation of the M3 subtype potentiates synaptic glycine release. Furthermore, GABA(B) receptors are involved in the feedback regulation of glycinergic synaptic transmission in the spinal cord. This study revealed distinct functions of mAChR subtypes in controlling glycinergic input to spinal dorsal horn neurons.

Authors+Show Affiliations

Department of Anesthesiology and Pain Medicine, Unit 110, The University of Texas MD Anderson Cancer Center, 1515 Holcombe Blvd., Houston, TX 77030-4009, USA.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, N.I.H., Extramural

Language

eng

PubMed ID

17878406

Citation

Zhang, Hong-Mei, et al. "Control of Glycinergic Input to Spinal Dorsal Horn Neurons By Distinct Muscarinic Receptor Subtypes Revealed Using Knockout Mice." The Journal of Pharmacology and Experimental Therapeutics, vol. 323, no. 3, 2007, pp. 963-71.
Zhang HM, Zhou HY, Chen SR, et al. Control of glycinergic input to spinal dorsal horn neurons by distinct muscarinic receptor subtypes revealed using knockout mice. J Pharmacol Exp Ther. 2007;323(3):963-71.
Zhang, H. M., Zhou, H. Y., Chen, S. R., Gautam, D., Wess, J., & Pan, H. L. (2007). Control of glycinergic input to spinal dorsal horn neurons by distinct muscarinic receptor subtypes revealed using knockout mice. The Journal of Pharmacology and Experimental Therapeutics, 323(3), 963-71.
Zhang HM, et al. Control of Glycinergic Input to Spinal Dorsal Horn Neurons By Distinct Muscarinic Receptor Subtypes Revealed Using Knockout Mice. J Pharmacol Exp Ther. 2007;323(3):963-71. PubMed PMID: 17878406.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Control of glycinergic input to spinal dorsal horn neurons by distinct muscarinic receptor subtypes revealed using knockout mice. AU - Zhang,Hong-Mei, AU - Zhou,Hong-Yi, AU - Chen,Shao-Rui, AU - Gautam,Dinesh, AU - Wess,Jürgen, AU - Pan,Hui-Lin, Y1 - 2007/09/18/ PY - 2007/9/20/pubmed PY - 2007/12/18/medline PY - 2007/9/20/entrez SP - 963 EP - 71 JF - The Journal of pharmacology and experimental therapeutics JO - J Pharmacol Exp Ther VL - 323 IS - 3 N2 - Muscarinic acetylcholine receptors (mAChRs) play an important role in the tonic regulation of nociceptive transmission in the spinal cord. However, how mAChR subtypes contribute to the regulation of synaptic glycine release is unknown. To determine their role, glycinergic spontaneous inhibitory postsynaptic currents (sIPSCs) were recorded in lamina II neurons by using whole-cell recordings in spinal cord slices of wild-type (WT) and mAChR subtype knockout (KO) mice. In WT mice, the mAChR agonist oxotremorine-M dose-dependently decreased the frequency of sIPSCs in most neurons, but it had variable effects in other neurons. In contrast, in M3-KO mice, oxotremorine-M consistently decreased the glycinergic sIPSC frequency in all neurons tested, and in M2/M4 double-KO mice, it always increased the sIPSC frequency. In M2/M4 double-KO mice, the potentiating effect of oxotremorine-M was attenuated by higher concentrations in some neurons through activation of GABA(B) receptors. In pertussis toxin-treated WT mice, oxotremorine-M also consistently increased the sIPSC frequency. In M2-KO and M4-KO mice, the effect of oxotremorine-M on sIPSCs was divergent because of the opposing functions of the M3 subtype and the M2 and M4 subtypes. This study demonstrates that stimulation of the M2 and M4 subtypes inhibits glycinergic inputs to spinal dorsal horn neurons of mice, whereas stimulation of the M3 subtype potentiates synaptic glycine release. Furthermore, GABA(B) receptors are involved in the feedback regulation of glycinergic synaptic transmission in the spinal cord. This study revealed distinct functions of mAChR subtypes in controlling glycinergic input to spinal dorsal horn neurons. SN - 1521-0103 UR - https://www.unboundmedicine.com/medline/citation/17878406/Control_of_glycinergic_input_to_spinal_dorsal_horn_neurons_by_distinct_muscarinic_receptor_subtypes_revealed_using_knockout_mice_ L2 - https://jpet.aspetjournals.org/cgi/pmidlookup?view=long&pmid=17878406 DB - PRIME DP - Unbound Medicine ER -