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Aptamer-mediated magnetic and gold-coated magnetic nanoparticles as detection assay for prion protein assessment.
Biotechnol Prog. 2007 Sep-Oct; 23(5):1239-44.BP

Abstract

This article reports the chemical synthesis and functionalization of magnetic and gold-coated magnetic nanoparticles. The binding characteristics of streptavidin-conjugated nanoparticles were studied using prion protein as a target to a specific biotinylated aptamer. The size and structure of the particles were determined by transmission electron microscopy, and the binding of the prion to the particle was confirmed by Fourier transform infrared spectroscopy. The rate of prion binding to the aptamer was dose-dependent, and prion immobilization was more effective on L-aspartic acid-functionalized magnetic nanoparticles compared to the carboxyl-functionalized gold-coated magnetic nanoparticles. This study sets the stage for the development of prion detection platforms as well as our long-term goals in structure elucidation at the binding interface.

Authors+Show Affiliations

Department of Agricultural and Biological Engineering, The Pennsylvania State University, University Park, Pennsylvania 16802, USA. gkk100@purdue.eduNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, U.S. Gov't, Non-P.H.S.

Language

eng

PubMed ID

17880236

Citation

Kouassi, Gilles K., et al. "Aptamer-mediated Magnetic and Gold-coated Magnetic Nanoparticles as Detection Assay for Prion Protein Assessment." Biotechnology Progress, vol. 23, no. 5, 2007, pp. 1239-44.
Kouassi GK, Wang P, Sreevatan S, et al. Aptamer-mediated magnetic and gold-coated magnetic nanoparticles as detection assay for prion protein assessment. Biotechnol Prog. 2007;23(5):1239-44.
Kouassi, G. K., Wang, P., Sreevatan, S., & Irudayaraj, J. (2007). Aptamer-mediated magnetic and gold-coated magnetic nanoparticles as detection assay for prion protein assessment. Biotechnology Progress, 23(5), 1239-44.
Kouassi GK, et al. Aptamer-mediated Magnetic and Gold-coated Magnetic Nanoparticles as Detection Assay for Prion Protein Assessment. Biotechnol Prog. 2007 Sep-Oct;23(5):1239-44. PubMed PMID: 17880236.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Aptamer-mediated magnetic and gold-coated magnetic nanoparticles as detection assay for prion protein assessment. AU - Kouassi,Gilles K, AU - Wang,Ping, AU - Sreevatan,Srinand, AU - Irudayaraj,Joseph, Y1 - 2007/09/20/ PY - 2007/9/21/pubmed PY - 2008/1/9/medline PY - 2007/9/21/entrez SP - 1239 EP - 44 JF - Biotechnology progress JO - Biotechnol Prog VL - 23 IS - 5 N2 - This article reports the chemical synthesis and functionalization of magnetic and gold-coated magnetic nanoparticles. The binding characteristics of streptavidin-conjugated nanoparticles were studied using prion protein as a target to a specific biotinylated aptamer. The size and structure of the particles were determined by transmission electron microscopy, and the binding of the prion to the particle was confirmed by Fourier transform infrared spectroscopy. The rate of prion binding to the aptamer was dose-dependent, and prion immobilization was more effective on L-aspartic acid-functionalized magnetic nanoparticles compared to the carboxyl-functionalized gold-coated magnetic nanoparticles. This study sets the stage for the development of prion detection platforms as well as our long-term goals in structure elucidation at the binding interface. SN - 8756-7938 UR - https://www.unboundmedicine.com/medline/citation/17880236/Aptamer_mediated_magnetic_and_gold_coated_magnetic_nanoparticles_as_detection_assay_for_prion_protein_assessment_ L2 - https://doi.org/10.1021/bp0602101 DB - PRIME DP - Unbound Medicine ER -