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Prevalence and quantitation of parvovirus B19 DNA levels in blood donors with a sensitive polymerase chain reaction screening assay.
Transfusion. 2007 Oct; 47(10):1756-64.T

Abstract

BACKGROUND

Blood donor parvovirus B19 DNA prevalence with sensitive nucleic acid test assays has recently been demonstrated to be higher than that found with assays designed to detect high viral titers in the plasma manufacturing sector.

STUDY DESIGN AND METHODS

Stored plasma aliquots from 5020 donations collected between 2000 and 2003 at seven US blood centers were tested. Testing was performed with a real-time B19 DNA polymerase chain reaction (PCR; TaqMan, Applied Biosystems) assay with a 50 percent limit of detection (LOD) of 1.6 IU per mL (95% confidence interval [CI], 1.2-2.1 IU/mL) and a 95 percent LOD of 16.5 IU per mL (95% CI, 10.6-33.9 IU/mL). Confirmation and quantitation of B19 DNA was accomplished by retesting of two additional subaliquots. Confirmed-positive specimens were tested for the presence of anti-B19 immunoglobulin M (IgM) and IgG with FDA-licensed assays.

RESULTS

B19 DNA prevalence was 0.88 percent (95% CI, 0.64%-1.2%). Among the 23 donations with B19 DNA titers of at least 20 IU per mL, the median DNA concentration was 105 IU per mL with an interquartile range of 42 to 481 IU per mL; the highest value was 1869 IU per mL. All B19 DNA-positive donations were positive for the presence of IgG and 10 (23%) were also positive for the presence of IgM; IgM seropositivity was associated with increasing DNA levels (p = 0.0013).

CONCLUSION

Low-level B19 DNA was detected in nearly 1 percent of donations. The 23 percent of DNA-positive donations with both IgM and IgG B19 antibody most likely represent acute resolving infection, whereas those with IgG but no IgM are most consistent with a more chronic and possibly persistent phase of B19 infection.

Authors+Show Affiliations

Westat, 1650 Research Boulevard, Rockville, MD 20850, USA.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, N.I.H., Extramural
Research Support, U.S. Gov't, P.H.S.

Language

eng

PubMed ID

17880600

Citation

Kleinman, Steven H., et al. "Prevalence and Quantitation of Parvovirus B19 DNA Levels in Blood Donors With a Sensitive Polymerase Chain Reaction Screening Assay." Transfusion, vol. 47, no. 10, 2007, pp. 1756-64.
Kleinman SH, Glynn SA, Lee TH, et al. Prevalence and quantitation of parvovirus B19 DNA levels in blood donors with a sensitive polymerase chain reaction screening assay. Transfusion. 2007;47(10):1756-64.
Kleinman, S. H., Glynn, S. A., Lee, T. H., Tobler, L., Montalvo, L., Todd, D., Kiss, J. E., Shyamala, V., & Busch, M. P. (2007). Prevalence and quantitation of parvovirus B19 DNA levels in blood donors with a sensitive polymerase chain reaction screening assay. Transfusion, 47(10), 1756-64.
Kleinman SH, et al. Prevalence and Quantitation of Parvovirus B19 DNA Levels in Blood Donors With a Sensitive Polymerase Chain Reaction Screening Assay. Transfusion. 2007;47(10):1756-64. PubMed PMID: 17880600.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Prevalence and quantitation of parvovirus B19 DNA levels in blood donors with a sensitive polymerase chain reaction screening assay. AU - Kleinman,Steven H, AU - Glynn,Simone A, AU - Lee,Tzong-Hae, AU - Tobler,Leslie, AU - Montalvo,Leilani, AU - Todd,Deborah, AU - Kiss,Joseph E, AU - Shyamala,Venkatakrishna, AU - Busch,Michael P, AU - ,, PY - 2007/9/21/pubmed PY - 2007/11/14/medline PY - 2007/9/21/entrez SP - 1756 EP - 64 JF - Transfusion JO - Transfusion VL - 47 IS - 10 N2 - BACKGROUND: Blood donor parvovirus B19 DNA prevalence with sensitive nucleic acid test assays has recently been demonstrated to be higher than that found with assays designed to detect high viral titers in the plasma manufacturing sector. STUDY DESIGN AND METHODS: Stored plasma aliquots from 5020 donations collected between 2000 and 2003 at seven US blood centers were tested. Testing was performed with a real-time B19 DNA polymerase chain reaction (PCR; TaqMan, Applied Biosystems) assay with a 50 percent limit of detection (LOD) of 1.6 IU per mL (95% confidence interval [CI], 1.2-2.1 IU/mL) and a 95 percent LOD of 16.5 IU per mL (95% CI, 10.6-33.9 IU/mL). Confirmation and quantitation of B19 DNA was accomplished by retesting of two additional subaliquots. Confirmed-positive specimens were tested for the presence of anti-B19 immunoglobulin M (IgM) and IgG with FDA-licensed assays. RESULTS: B19 DNA prevalence was 0.88 percent (95% CI, 0.64%-1.2%). Among the 23 donations with B19 DNA titers of at least 20 IU per mL, the median DNA concentration was 105 IU per mL with an interquartile range of 42 to 481 IU per mL; the highest value was 1869 IU per mL. All B19 DNA-positive donations were positive for the presence of IgG and 10 (23%) were also positive for the presence of IgM; IgM seropositivity was associated with increasing DNA levels (p = 0.0013). CONCLUSION: Low-level B19 DNA was detected in nearly 1 percent of donations. The 23 percent of DNA-positive donations with both IgM and IgG B19 antibody most likely represent acute resolving infection, whereas those with IgG but no IgM are most consistent with a more chronic and possibly persistent phase of B19 infection. SN - 0041-1132 UR - https://www.unboundmedicine.com/medline/citation/17880600/Prevalence_and_quantitation_of_parvovirus_B19_DNA_levels_in_blood_donors_with_a_sensitive_polymerase_chain_reaction_screening_assay_ L2 - https://doi.org/10.1111/j.1537-2995.2007.01341.x DB - PRIME DP - Unbound Medicine ER -