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Effects of scutellarin on PKCgamma in PC12 cell injury induced by oxygen and glucose deprivation.
Acta Pharmacol Sin. 2007 Oct; 28(10):1573-9.AP

Abstract

AIM

To evaluate the neuroprotective effect and mechanisms of scutellarin (Scu) against PC12 cell injury after oxygen and glucose deprivation followed by reperfusion (OGD-Rep).

METHODS

Undifferentiated rat pheochromocytoma PC12 cells, exposed to oxygen and glucose deprivation followed by reperfusion (OGD-Rep), used as an in vitro model of ischemia/reperfusion. Cell survival was evaluated by diphenyltetrazolium bromide (MTT) assay and the amount of LDH release was determined using assay kits. [Ca2+](i) was monitored using a fluorescent Ca2+-sensitive dye Fura-2 acetoxymethyl ester. Cell apoptosis was detected by a DNA ladder and by flow cytometric detection. The expression of protein kinase C (PKC)gamma was determined using both RT-PCR and Western blotting. The translocation of PKCgamma was assayed by subcellular fractionation and Western blotting.

RESULTS

OGD-Rep injury significantly elevated the level of LDH release, [Ca2+](i), mRNA expression and the translocation of PKCgamma compared in the PC12 cells with those of the normal group. Scu (10-100 micromol/L) exerted a protective effect against OGD-Rep injury by reducing LDH release, [Ca2+](i), the percent of apoptosis, and the translocation of PKCgamma.

CONCLUSION

Scu inhibits the increase of [Ca2+](i) and the activation of PKCgamma, exerting protective effects against PC12 cell injury induced by OGD-Rep.

Authors+Show Affiliations

State Key Laboratory of Drug Research, Shanghai Institute of Materia Medica, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai 201203, China.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

17883942

Citation

Xu, Wei, et al. "Effects of Scutellarin On PKCgamma in PC12 Cell Injury Induced By Oxygen and Glucose Deprivation." Acta Pharmacologica Sinica, vol. 28, no. 10, 2007, pp. 1573-9.
Xu W, Zha RP, Wang WY, et al. Effects of scutellarin on PKCgamma in PC12 cell injury induced by oxygen and glucose deprivation. Acta Pharmacol Sin. 2007;28(10):1573-9.
Xu, W., Zha, R. P., Wang, W. Y., & Wang, Y. P. (2007). Effects of scutellarin on PKCgamma in PC12 cell injury induced by oxygen and glucose deprivation. Acta Pharmacologica Sinica, 28(10), 1573-9.
Xu W, et al. Effects of Scutellarin On PKCgamma in PC12 Cell Injury Induced By Oxygen and Glucose Deprivation. Acta Pharmacol Sin. 2007;28(10):1573-9. PubMed PMID: 17883942.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Effects of scutellarin on PKCgamma in PC12 cell injury induced by oxygen and glucose deprivation. AU - Xu,Wei, AU - Zha,Ruo-Peng, AU - Wang,Wen-Yi, AU - Wang,Yi-Ping, PY - 2007/9/22/pubmed PY - 2008/11/19/medline PY - 2007/9/22/entrez SP - 1573 EP - 9 JF - Acta pharmacologica Sinica JO - Acta Pharmacol. Sin. VL - 28 IS - 10 N2 - AIM: To evaluate the neuroprotective effect and mechanisms of scutellarin (Scu) against PC12 cell injury after oxygen and glucose deprivation followed by reperfusion (OGD-Rep). METHODS: Undifferentiated rat pheochromocytoma PC12 cells, exposed to oxygen and glucose deprivation followed by reperfusion (OGD-Rep), used as an in vitro model of ischemia/reperfusion. Cell survival was evaluated by diphenyltetrazolium bromide (MTT) assay and the amount of LDH release was determined using assay kits. [Ca2+](i) was monitored using a fluorescent Ca2+-sensitive dye Fura-2 acetoxymethyl ester. Cell apoptosis was detected by a DNA ladder and by flow cytometric detection. The expression of protein kinase C (PKC)gamma was determined using both RT-PCR and Western blotting. The translocation of PKCgamma was assayed by subcellular fractionation and Western blotting. RESULTS: OGD-Rep injury significantly elevated the level of LDH release, [Ca2+](i), mRNA expression and the translocation of PKCgamma compared in the PC12 cells with those of the normal group. Scu (10-100 micromol/L) exerted a protective effect against OGD-Rep injury by reducing LDH release, [Ca2+](i), the percent of apoptosis, and the translocation of PKCgamma. CONCLUSION: Scu inhibits the increase of [Ca2+](i) and the activation of PKCgamma, exerting protective effects against PC12 cell injury induced by OGD-Rep. SN - 1671-4083 UR - https://www.unboundmedicine.com/medline/citation/17883942/Effects_of_scutellarin_on_PKCgamma_in_PC12_cell_injury_induced_by_oxygen_and_glucose_deprivation_ L2 - http://dx.doi.org/10.1111/j.1745-7254.2007.00502.x DB - PRIME DP - Unbound Medicine ER -