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Adenovirus vector-mediated ex vivo gene transfer of brain-derived neurotrophic factor to bone marrow stromal cells promotes axonal regeneration after transplantation in completely transected adult rat spinal cord.
Eur Spine J. 2007 Dec; 16(12):2206-14.ES

Abstract

The aim of this study was to evaluate the efficacy in adult rat completely transected spinal cord of adenovirus vector-mediated brain-derived neurotrophic factor (BDNF) ex vivo gene transfer to bone marrow stromal cells (BMSC). BMSC were infected with adenovirus vectors carrying beta-galactosidase (AxCALacZ) or BDNF (AxCABDNF) genes. The T8 segment of spinal cord was removed and replaced by graft containing Matrigel alone (MG group) or Matrigel and BMSC infected by AxCALacZ (BMSC-LacZ group) or AxCABDNF (BMSC-BDNF group). Axons in the graft were evaluated by immunohistochemistry and functional recovery was assessed with BBB locomotor scale. In the BMSC-BDNF group, the number of fibers positive for growth associated protein-43, tyrosine hydroxylase, and calcitonin gene-related peptide was significantly larger than numbers found for the MG and BMSC-LacZ groups. Rats from BMSC-BDNF and BMSC-LacZ groups showed significant recovery of hind limb function compared with MG rats; however, there was no significant difference between groups in degree of functional recovery. These findings demonstrate that adenovirus vector-mediated ex vivo gene transfer of BDNF enhances the capacity of BMSC to promote axonal regeneration in this completely transected spinal cord model; however, BDNF failed to enhance hind limb functional recovery. Further investigation is needed to establish an optimal combination of cell therapy and neurotrophin gene transfer for cases of spinal cord injury.

Authors+Show Affiliations

Department of Orthopaedic Surgery, Chiba University Graduate School of Medicine, 1-8-1 Inohana, Chuo-ku, Chiba, 260-8670, Japan. m-koda@bb.em-net.ne.jpNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

17885772

Citation

Koda, Masao, et al. "Adenovirus Vector-mediated Ex Vivo Gene Transfer of Brain-derived Neurotrophic Factor to Bone Marrow Stromal Cells Promotes Axonal Regeneration After Transplantation in Completely Transected Adult Rat Spinal Cord." European Spine Journal : Official Publication of the European Spine Society, the European Spinal Deformity Society, and the European Section of the Cervical Spine Research Society, vol. 16, no. 12, 2007, pp. 2206-14.
Koda M, Kamada T, Hashimoto M, et al. Adenovirus vector-mediated ex vivo gene transfer of brain-derived neurotrophic factor to bone marrow stromal cells promotes axonal regeneration after transplantation in completely transected adult rat spinal cord. Eur Spine J. 2007;16(12):2206-14.
Koda, M., Kamada, T., Hashimoto, M., Murakami, M., Shirasawa, H., Sakao, S., Ino, H., Yoshinaga, K., Koshizuka, S., Moriya, H., & Yamazaki, M. (2007). Adenovirus vector-mediated ex vivo gene transfer of brain-derived neurotrophic factor to bone marrow stromal cells promotes axonal regeneration after transplantation in completely transected adult rat spinal cord. European Spine Journal : Official Publication of the European Spine Society, the European Spinal Deformity Society, and the European Section of the Cervical Spine Research Society, 16(12), 2206-14.
Koda M, et al. Adenovirus Vector-mediated Ex Vivo Gene Transfer of Brain-derived Neurotrophic Factor to Bone Marrow Stromal Cells Promotes Axonal Regeneration After Transplantation in Completely Transected Adult Rat Spinal Cord. Eur Spine J. 2007;16(12):2206-14. PubMed PMID: 17885772.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Adenovirus vector-mediated ex vivo gene transfer of brain-derived neurotrophic factor to bone marrow stromal cells promotes axonal regeneration after transplantation in completely transected adult rat spinal cord. AU - Koda,Masao, AU - Kamada,Takahito, AU - Hashimoto,Masayuki, AU - Murakami,Masazumi, AU - Shirasawa,Hiroshi, AU - Sakao,Seiichiro, AU - Ino,Hidetoshi, AU - Yoshinaga,Katsunori, AU - Koshizuka,Shuhei, AU - Moriya,Hideshige, AU - Yamazaki,Masashi, Y1 - 2007/09/21/ PY - 2007/01/08/received PY - 2007/08/28/accepted PY - 2007/06/01/revised PY - 2007/9/22/pubmed PY - 2008/1/30/medline PY - 2007/9/22/entrez SP - 2206 EP - 14 JF - European spine journal : official publication of the European Spine Society, the European Spinal Deformity Society, and the European Section of the Cervical Spine Research Society JO - Eur Spine J VL - 16 IS - 12 N2 - The aim of this study was to evaluate the efficacy in adult rat completely transected spinal cord of adenovirus vector-mediated brain-derived neurotrophic factor (BDNF) ex vivo gene transfer to bone marrow stromal cells (BMSC). BMSC were infected with adenovirus vectors carrying beta-galactosidase (AxCALacZ) or BDNF (AxCABDNF) genes. The T8 segment of spinal cord was removed and replaced by graft containing Matrigel alone (MG group) or Matrigel and BMSC infected by AxCALacZ (BMSC-LacZ group) or AxCABDNF (BMSC-BDNF group). Axons in the graft were evaluated by immunohistochemistry and functional recovery was assessed with BBB locomotor scale. In the BMSC-BDNF group, the number of fibers positive for growth associated protein-43, tyrosine hydroxylase, and calcitonin gene-related peptide was significantly larger than numbers found for the MG and BMSC-LacZ groups. Rats from BMSC-BDNF and BMSC-LacZ groups showed significant recovery of hind limb function compared with MG rats; however, there was no significant difference between groups in degree of functional recovery. These findings demonstrate that adenovirus vector-mediated ex vivo gene transfer of BDNF enhances the capacity of BMSC to promote axonal regeneration in this completely transected spinal cord model; however, BDNF failed to enhance hind limb functional recovery. Further investigation is needed to establish an optimal combination of cell therapy and neurotrophin gene transfer for cases of spinal cord injury. SN - 1432-0932 UR - https://www.unboundmedicine.com/medline/citation/17885772/Adenovirus_vector_mediated_ex_vivo_gene_transfer_of_brain_derived_neurotrophic_factor_to_bone_marrow_stromal_cells_promotes_axonal_regeneration_after_transplantation_in_completely_transected_adult_rat_spinal_cord_ L2 - https://doi.org/10.1007/s00586-007-0499-3 DB - PRIME DP - Unbound Medicine ER -