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Evaluation of the Panbio dengue virus nonstructural 1 antigen detection and immunoglobulin M antibody enzyme-linked immunosorbent assays for the diagnosis of acute dengue infections in Laos.
Diagn Microbiol Infect Dis. 2008 Jan; 60(1):43-9.DM

Abstract

We evaluated 2 commercial enzyme-linked immunosorbent assays (ELISAs) for the diagnosis of dengue infection; one a serologic test for immunoglobulin M (IgM) antibodies, the other based on detection of dengue virus nonstructural 1 (NS1) antigen. Using gold standard reference serology on paired sera, 41% (38/92 patients) were dengue confirmed, with 4 (11%) acute primary and 33 (87%) acute secondary infections (1 was of indeterminate status). Sensitivity of the NS1-ELISA was 63% (95% confidence interval [CI], 53-73) on admission samples but was much less sensitive (5%; 95% CI, 1-10) on convalescent samples. The IgM capture ELISA had a lower but statistically equivalent sensitivity compared with the NS1-ELISA for admission samples (45%; 95% CI, 35-55) but was more sensitive on convalescent samples (58%; 95% CI, 48-68). The results of the NS1 and IgM capture ELISAs were combined using a logical OR operator, increasing the sensitivity for admission samples (79%; 95% CI, 71-87), convalescent samples (63%; 95% CI, 53-73), and all samples (71%; 95% CI, 65-78).

Authors+Show Affiliations

Wellcome Trust-Mahosot Hospital-Oxford Tropical Medicine Research Collaboration, Microbiology Laboratory, Mahosot Hospital, Vientiane, Lao PDR. stuart@tropmedres.acNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Evaluation Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

17889487

Citation

Blacksell, Stuart D., et al. "Evaluation of the Panbio Dengue Virus Nonstructural 1 Antigen Detection and Immunoglobulin M Antibody Enzyme-linked Immunosorbent Assays for the Diagnosis of Acute Dengue Infections in Laos." Diagnostic Microbiology and Infectious Disease, vol. 60, no. 1, 2008, pp. 43-9.
Blacksell SD, Mammen MP, Thongpaseuth S, et al. Evaluation of the Panbio dengue virus nonstructural 1 antigen detection and immunoglobulin M antibody enzyme-linked immunosorbent assays for the diagnosis of acute dengue infections in Laos. Diagn Microbiol Infect Dis. 2008;60(1):43-9.
Blacksell, S. D., Mammen, M. P., Thongpaseuth, S., Gibbons, R. V., Jarman, R. G., Jenjaroen, K., Nisalak, A., Phetsouvanh, R., Newton, P. N., & Day, N. P. (2008). Evaluation of the Panbio dengue virus nonstructural 1 antigen detection and immunoglobulin M antibody enzyme-linked immunosorbent assays for the diagnosis of acute dengue infections in Laos. Diagnostic Microbiology and Infectious Disease, 60(1), 43-9.
Blacksell SD, et al. Evaluation of the Panbio Dengue Virus Nonstructural 1 Antigen Detection and Immunoglobulin M Antibody Enzyme-linked Immunosorbent Assays for the Diagnosis of Acute Dengue Infections in Laos. Diagn Microbiol Infect Dis. 2008;60(1):43-9. PubMed PMID: 17889487.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Evaluation of the Panbio dengue virus nonstructural 1 antigen detection and immunoglobulin M antibody enzyme-linked immunosorbent assays for the diagnosis of acute dengue infections in Laos. AU - Blacksell,Stuart D, AU - Mammen,Mammen P,Jr AU - Thongpaseuth,Soulignasack, AU - Gibbons,Robert V, AU - Jarman,Richard G, AU - Jenjaroen,Kemajittra, AU - Nisalak,Ananda, AU - Phetsouvanh,Rattanaphone, AU - Newton,Paul N, AU - Day,Nicholas P J, Y1 - 2007/09/24/ PY - 2007/01/16/received PY - 2007/07/22/revised PY - 2007/07/23/accepted PY - 2007/9/25/pubmed PY - 2008/4/11/medline PY - 2007/9/25/entrez SP - 43 EP - 9 JF - Diagnostic microbiology and infectious disease JO - Diagn Microbiol Infect Dis VL - 60 IS - 1 N2 - We evaluated 2 commercial enzyme-linked immunosorbent assays (ELISAs) for the diagnosis of dengue infection; one a serologic test for immunoglobulin M (IgM) antibodies, the other based on detection of dengue virus nonstructural 1 (NS1) antigen. Using gold standard reference serology on paired sera, 41% (38/92 patients) were dengue confirmed, with 4 (11%) acute primary and 33 (87%) acute secondary infections (1 was of indeterminate status). Sensitivity of the NS1-ELISA was 63% (95% confidence interval [CI], 53-73) on admission samples but was much less sensitive (5%; 95% CI, 1-10) on convalescent samples. The IgM capture ELISA had a lower but statistically equivalent sensitivity compared with the NS1-ELISA for admission samples (45%; 95% CI, 35-55) but was more sensitive on convalescent samples (58%; 95% CI, 48-68). The results of the NS1 and IgM capture ELISAs were combined using a logical OR operator, increasing the sensitivity for admission samples (79%; 95% CI, 71-87), convalescent samples (63%; 95% CI, 53-73), and all samples (71%; 95% CI, 65-78). SN - 0732-8893 UR - https://www.unboundmedicine.com/medline/citation/17889487/Evaluation_of_the_Panbio_dengue_virus_nonstructural_1_antigen_detection_and_immunoglobulin_M_antibody_enzyme_linked_immunosorbent_assays_for_the_diagnosis_of_acute_dengue_infections_in_Laos_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0732-8893(07)00332-X DB - PRIME DP - Unbound Medicine ER -