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In vivo genotoxicity of mercury chloride and lead acetate: Micronucleus test on acridine orange stained fish cells.
Food Chem Toxicol. 2008 Jan; 46(1):352-8.FC

Abstract

The genotoxic effects of mercury chloride and lead acetate were evaluated in vivo using the micronucleus (MN) assay on acridine-orange (AO) stained peripheral blood erythrocytes, gill and fin epithelial cells of Carassius auratus auratus. Fish were exposed to three different concentrations of mercury chloride (MC) (1 microg/, 5 microg/L and 10 microg/L) and lead acetate (LA) (10 microg/L, 50 microg/L and 100 microg/L) for 2, 4 and 6 days. A single dose of 5 mg/L cyclophosphamide was used as a positive control. In addition to micronuclei, nuclear buds (NBs) were assessed in the erythrocytes. The ratio of polychromatic and normochromatic erythrocytes (PCE/NCE) in peripheral blood was also evaluated to assess cytotoxicity. MN frequencies in all three tissues were elevated in fish exposed to both LA and MC. However, NBs showed different sensitivity to metal treatments. MN frequencies in both control and treated fish were highest in gill cells and generally lower in erythrocytes and fin cells. PCE/NCE rations decreased in relation to MC and LA treatments. The results of this study indicate that LA and MC have genotoxic and cytotoxic damage in fish and confirmed that AO staining is a suitable technique for in vivo MN test in fish.

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Authors+Show Affiliations

Cavaş T
Mersin University, Faculty of Sciences and Letters, Department of Biology, 33342 Mersin, Turkey. tcavas@mersin.edu.tr

MeSH

Acridine OrangeAnimalsAzure StainsCells, CulturedErythrocytesGillsGoldfishMercuric ChlorideMicronucleus TestsMicroscopy, FluorescenceMutagensOrganometallic Compounds

Pub Type(s)

Journal Article

Language

eng

PubMed ID

17889980

Citation

Cavaş, Tolga. "In Vivo Genotoxicity of Mercury Chloride and Lead Acetate: Micronucleus Test On Acridine Orange Stained Fish Cells." Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association, vol. 46, no. 1, 2008, pp. 352-8.
Cavaş T. In vivo genotoxicity of mercury chloride and lead acetate: Micronucleus test on acridine orange stained fish cells. Food Chem Toxicol. 2008;46(1):352-8.
Cavaş, T. (2008). In vivo genotoxicity of mercury chloride and lead acetate: Micronucleus test on acridine orange stained fish cells. Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association, 46(1), 352-8.
Cavaş T. In Vivo Genotoxicity of Mercury Chloride and Lead Acetate: Micronucleus Test On Acridine Orange Stained Fish Cells. Food Chem Toxicol. 2008;46(1):352-8. PubMed PMID: 17889980.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - In vivo genotoxicity of mercury chloride and lead acetate: Micronucleus test on acridine orange stained fish cells. A1 - Cavaş,Tolga, Y1 - 2007/08/21/ PY - 2006/12/22/received PY - 2007/06/01/revised PY - 2007/08/10/accepted PY - 2007/9/25/pubmed PY - 2008/3/12/medline PY - 2007/9/25/entrez SP - 352 EP - 8 JF - Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association JO - Food Chem Toxicol VL - 46 IS - 1 N2 - The genotoxic effects of mercury chloride and lead acetate were evaluated in vivo using the micronucleus (MN) assay on acridine-orange (AO) stained peripheral blood erythrocytes, gill and fin epithelial cells of Carassius auratus auratus. Fish were exposed to three different concentrations of mercury chloride (MC) (1 microg/, 5 microg/L and 10 microg/L) and lead acetate (LA) (10 microg/L, 50 microg/L and 100 microg/L) for 2, 4 and 6 days. A single dose of 5 mg/L cyclophosphamide was used as a positive control. In addition to micronuclei, nuclear buds (NBs) were assessed in the erythrocytes. The ratio of polychromatic and normochromatic erythrocytes (PCE/NCE) in peripheral blood was also evaluated to assess cytotoxicity. MN frequencies in all three tissues were elevated in fish exposed to both LA and MC. However, NBs showed different sensitivity to metal treatments. MN frequencies in both control and treated fish were highest in gill cells and generally lower in erythrocytes and fin cells. PCE/NCE rations decreased in relation to MC and LA treatments. The results of this study indicate that LA and MC have genotoxic and cytotoxic damage in fish and confirmed that AO staining is a suitable technique for in vivo MN test in fish. SN - 0278-6915 UR - https://www.unboundmedicine.com/medline/citation/17889980/In_vivo_genotoxicity_of_mercury_chloride_and_lead_acetate:_Micronucleus_test_on_acridine_orange_stained_fish_cells_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0278-6915(07)00302-X DB - PRIME DP - Unbound Medicine ER -