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Helicobacter pylori-associated gastritis is related to babA2 expression without heterogeneity of the 3' region of the cagA genotype in gastric biopsy specimens.
Pathobiology. 2007; 74(5):309-16.P

Abstract

BACKGROUND

Cytotoxin-associated gene A (cagA) is the major virulence factor of Helicobacter pylori strains and affects the clinical outcome of patients. Blood group antigen binding adhesin (BabA) helps the strains adhere to the epithelial cell layer and is the most important adhesin of H. pylori.

OBJECTIVES

We tried to study the association between the status of babA2 and cagA in H. pylori strains and histological gastritis. methods: Thirty-six patients were included. RNA was extracted from two frozen biopsy samples of the antrum and corpus, respectively, and cagA/babA2 genotypes were analyzed with reverse transcription polymerase chain reaction and direct sequencing. Two gastric specimens of the antrum and corpus, respectively, were also stained with hematoxylin and eosin to analyze H. pylori-related gastritis.

RESULTS

In the antrum, 56% of the specimens were babA2 positive and in the corpus 53%. The gastritis scores of activity and inflammation were associated with the presence of babA2 in antrum specimens but not in corpus specimens. cagA gene encoding in the CagA EPIYA-D region was detected in all samples, and the sequence was completely identical between those from the gastric corpus and antrum.

CONCLUSION

babA2 expression is heterogeneous and correlated with the extent of gastritis in the antrum, but not in the corpus, whereas cagA shows a monotonous genotype.

Authors+Show Affiliations

Department of Medicine and Molecular Science, Hiroshima University, Hiroshima, Japan.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

17890898

Citation

Xie, X F., et al. "Helicobacter Pylori-associated Gastritis Is Related to babA2 Expression Without Heterogeneity of the 3' Region of the cagA Genotype in Gastric Biopsy Specimens." Pathobiology : Journal of Immunopathology, Molecular and Cellular Biology, vol. 74, no. 5, 2007, pp. 309-16.
Xie XF, Ito M, Sumii M, et al. Helicobacter pylori-associated gastritis is related to babA2 expression without heterogeneity of the 3' region of the cagA genotype in gastric biopsy specimens. Pathobiology. 2007;74(5):309-16.
Xie, X. F., Ito, M., Sumii, M., Tanaka, S., Yoshihara, M., & Chayama, K. (2007). Helicobacter pylori-associated gastritis is related to babA2 expression without heterogeneity of the 3' region of the cagA genotype in gastric biopsy specimens. Pathobiology : Journal of Immunopathology, Molecular and Cellular Biology, 74(5), 309-16.
Xie XF, et al. Helicobacter Pylori-associated Gastritis Is Related to babA2 Expression Without Heterogeneity of the 3' Region of the cagA Genotype in Gastric Biopsy Specimens. Pathobiology. 2007;74(5):309-16. PubMed PMID: 17890898.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Helicobacter pylori-associated gastritis is related to babA2 expression without heterogeneity of the 3' region of the cagA genotype in gastric biopsy specimens. AU - Xie,X F, AU - Ito,M, AU - Sumii,M, AU - Tanaka,S, AU - Yoshihara,M, AU - Chayama,K, PY - 2006/11/03/received PY - 2007/01/15/accepted PY - 2007/9/25/pubmed PY - 2007/12/28/medline PY - 2007/9/25/entrez SP - 309 EP - 16 JF - Pathobiology : journal of immunopathology, molecular and cellular biology JO - Pathobiology VL - 74 IS - 5 N2 - BACKGROUND: Cytotoxin-associated gene A (cagA) is the major virulence factor of Helicobacter pylori strains and affects the clinical outcome of patients. Blood group antigen binding adhesin (BabA) helps the strains adhere to the epithelial cell layer and is the most important adhesin of H. pylori. OBJECTIVES: We tried to study the association between the status of babA2 and cagA in H. pylori strains and histological gastritis. methods: Thirty-six patients were included. RNA was extracted from two frozen biopsy samples of the antrum and corpus, respectively, and cagA/babA2 genotypes were analyzed with reverse transcription polymerase chain reaction and direct sequencing. Two gastric specimens of the antrum and corpus, respectively, were also stained with hematoxylin and eosin to analyze H. pylori-related gastritis. RESULTS: In the antrum, 56% of the specimens were babA2 positive and in the corpus 53%. The gastritis scores of activity and inflammation were associated with the presence of babA2 in antrum specimens but not in corpus specimens. cagA gene encoding in the CagA EPIYA-D region was detected in all samples, and the sequence was completely identical between those from the gastric corpus and antrum. CONCLUSION: babA2 expression is heterogeneous and correlated with the extent of gastritis in the antrum, but not in the corpus, whereas cagA shows a monotonous genotype. SN - 1423-0291 UR - https://www.unboundmedicine.com/medline/citation/17890898/Helicobacter_pylori_associated_gastritis_is_related_to_babA2_expression_without_heterogeneity_of_the_3'_region_of_the_cagA_genotype_in_gastric_biopsy_specimens_ L2 - https://www.karger.com?DOI=10.1159/000105814 DB - PRIME DP - Unbound Medicine ER -