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An ecotoxicological protocol with caged mussels, Mytilus galloprovincialis, for monitoring the impact of an offshore platform in the Adriatic Sea.
Mar Environ Res. 2008 Feb; 65(1):34-49.ME

Abstract

An ecotoxicological protocol with caged mussels, Mytilus galloprovincialis, was developed to evaluate the potential impact of an offshore gas platform in the central Adriatic Sea. Reference organisms were collected on a seasonal basis from an unpolluted site and transplanted for four weeks in both the sampling area and to the investigated platform. Chemical analyses of trace metals in mussel tissues were integrated with a multi-biomarker approach for the early detection of biological responses at several cellular targets. Induction of metallothioneins, peroxisomal proliferation and activity of acetylcholinesterase were measured as markers for specific classes of chemicals. Special attention was given to oxyradical metabolism and appearance of oxidative-mediated toxicity to reveal a more general onset of cellular disturbance. In addition to individual antioxidants (superoxide dismutase, catalase, glutathione S-transferases, glutathione reductase, Se-dependent and Se-independent glutathione peroxidases, and levels of total glutathione), the total oxyradical scavenging capacity (TOSC) allowed a quantification of the overall capability to neutralize specific forms of intracellular reactive oxygen species (ROS; i.e. peroxyl and hydroxyl radicals). Cellular damages were evaluated as lysosomal destabilization (membrane stability, accumulation of lipofuscin and neutral lipids), lipid peroxidation products (malondialdehyde) and DNA integrity (strand breaks and micronuclei); the air survival test was finally applied to evaluate the overall physiological condition of mussels. Concentration of trace metals (As, Ba, Cd, Cr, Cu, Fe, Hg, Mn, Ni, Pb, Zn) revealed only limited variations in transplanted mussels during various experimental periods and such changes appeared partly related to natural fluctuations. Among biological responses, variations of antioxidants and lysosomal stability were confirmed as sensitive early warning signals for biological disturbance of both natural and anthropogenic origin. The presented protocol with caged mussels allowed marked biological effects caused by the investigated platform to be excluded, and represented a useful approach that is easy to extend for monitoring the impact of offshore activities in the Adriatic sea.

Authors+Show Affiliations

Istituto di Biologia e Genetica, Università Politecnica delle Marche, Via Ranieri Monte d'Ago, 60100 Ancona, Italy.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

17897709

Citation

Gorbi, Stefania, et al. "An Ecotoxicological Protocol With Caged Mussels, Mytilus Galloprovincialis, for Monitoring the Impact of an Offshore Platform in the Adriatic Sea." Marine Environmental Research, vol. 65, no. 1, 2008, pp. 34-49.
Gorbi S, Virno Lamberti C, Notti A, et al. An ecotoxicological protocol with caged mussels, Mytilus galloprovincialis, for monitoring the impact of an offshore platform in the Adriatic Sea. Mar Environ Res. 2008;65(1):34-49.
Gorbi, S., Virno Lamberti, C., Notti, A., Benedetti, M., Fattorini, D., Moltedo, G., & Regoli, F. (2008). An ecotoxicological protocol with caged mussels, Mytilus galloprovincialis, for monitoring the impact of an offshore platform in the Adriatic Sea. Marine Environmental Research, 65(1), 34-49.
Gorbi S, et al. An Ecotoxicological Protocol With Caged Mussels, Mytilus Galloprovincialis, for Monitoring the Impact of an Offshore Platform in the Adriatic Sea. Mar Environ Res. 2008;65(1):34-49. PubMed PMID: 17897709.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - An ecotoxicological protocol with caged mussels, Mytilus galloprovincialis, for monitoring the impact of an offshore platform in the Adriatic Sea. AU - Gorbi,Stefania, AU - Virno Lamberti,Claudia, AU - Notti,Alessandra, AU - Benedetti,Maura, AU - Fattorini,Daniele, AU - Moltedo,Ginevra, AU - Regoli,Francesco, Y1 - 2007/08/23/ PY - 2007/03/15/received PY - 2007/07/12/revised PY - 2007/07/16/accepted PY - 2007/9/28/pubmed PY - 2008/3/19/medline PY - 2007/9/28/entrez SP - 34 EP - 49 JF - Marine environmental research JO - Mar Environ Res VL - 65 IS - 1 N2 - An ecotoxicological protocol with caged mussels, Mytilus galloprovincialis, was developed to evaluate the potential impact of an offshore gas platform in the central Adriatic Sea. Reference organisms were collected on a seasonal basis from an unpolluted site and transplanted for four weeks in both the sampling area and to the investigated platform. Chemical analyses of trace metals in mussel tissues were integrated with a multi-biomarker approach for the early detection of biological responses at several cellular targets. Induction of metallothioneins, peroxisomal proliferation and activity of acetylcholinesterase were measured as markers for specific classes of chemicals. Special attention was given to oxyradical metabolism and appearance of oxidative-mediated toxicity to reveal a more general onset of cellular disturbance. In addition to individual antioxidants (superoxide dismutase, catalase, glutathione S-transferases, glutathione reductase, Se-dependent and Se-independent glutathione peroxidases, and levels of total glutathione), the total oxyradical scavenging capacity (TOSC) allowed a quantification of the overall capability to neutralize specific forms of intracellular reactive oxygen species (ROS; i.e. peroxyl and hydroxyl radicals). Cellular damages were evaluated as lysosomal destabilization (membrane stability, accumulation of lipofuscin and neutral lipids), lipid peroxidation products (malondialdehyde) and DNA integrity (strand breaks and micronuclei); the air survival test was finally applied to evaluate the overall physiological condition of mussels. Concentration of trace metals (As, Ba, Cd, Cr, Cu, Fe, Hg, Mn, Ni, Pb, Zn) revealed only limited variations in transplanted mussels during various experimental periods and such changes appeared partly related to natural fluctuations. Among biological responses, variations of antioxidants and lysosomal stability were confirmed as sensitive early warning signals for biological disturbance of both natural and anthropogenic origin. The presented protocol with caged mussels allowed marked biological effects caused by the investigated platform to be excluded, and represented a useful approach that is easy to extend for monitoring the impact of offshore activities in the Adriatic sea. SN - 0141-1136 UR - https://www.unboundmedicine.com/medline/citation/17897709/An_ecotoxicological_protocol_with_caged_mussels_Mytilus_galloprovincialis_for_monitoring_the_impact_of_an_offshore_platform_in_the_Adriatic_Sea_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0141-1136(07)00093-1 DB - PRIME DP - Unbound Medicine ER -