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Lack of association between CpG island methylator phenotype in human gastric cancers and methylation in their background non-cancerous gastric mucosae.
Cancer Sci. 2007 Dec; 98(12):1853-61.CS

Abstract

The presence of high levels of aberrant DNA methylation in gastric mucosae correlates with risk of gastric cancer. Some gastric cancers are known to have methylation of multiple CpG islands (CGI), which is referred to as the CGI methylator phenotype (CIMP). In the present study, we aimed to clarify the possible association between the CIMP in cancers and high methylation levels in their background mucosae by accurate quantitative methylation analysis of 14 carefully selected promoter CGI. Methylation levels were measured in 66 cancers and their background mucosae, along with 19 normal mucosae of healthy volunteers. Methylation in cancers was classified as absent (methylation level = 0%) or positive. The number of methylated CGI in a cancer showed a continuous distribution, and cancers were classified as CIMP high (21 cases), CIMP low (30 cases), or CIMP negative (15 cases). CIMP-high gastric cancer patients had significantly better survival rates than CIMP-negative patients. Of the Epstein-Barr virus-positive gastric cancers studied, eight out of nine presented as CIMP high. Methylation in background mucosae showed a unimodal distribution, and was assessed by their degree. The gastric mucosae of cancer patients showed higher levels than normal gastric mucosae of healthy volunteers. Finally, the CIMP-high, CIMP-low, and CIMP-negative statuses in cancers were not associated with methylation levels of individual genes and their means in the background mucosae. These showed that the CIMP statuses in gastric cancers had no association with methylation levels in the background gastric mucosae.

Authors+Show Affiliations

Carcinogenesis Division, National Cancer Center Research Institute, 5-1-1 Tsukiji, Chuo-ku, Tokyo 104-0045, Japan.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

17900260

Citation

Enomoto, Shotaro, et al. "Lack of Association Between CpG Island Methylator Phenotype in Human Gastric Cancers and Methylation in Their Background Non-cancerous Gastric Mucosae." Cancer Science, vol. 98, no. 12, 2007, pp. 1853-61.
Enomoto S, Maekita T, Tsukamoto T, et al. Lack of association between CpG island methylator phenotype in human gastric cancers and methylation in their background non-cancerous gastric mucosae. Cancer Sci. 2007;98(12):1853-61.
Enomoto, S., Maekita, T., Tsukamoto, T., Nakajima, T., Nakazawa, K., Tatematsu, M., Ichinose, M., & Ushijima, T. (2007). Lack of association between CpG island methylator phenotype in human gastric cancers and methylation in their background non-cancerous gastric mucosae. Cancer Science, 98(12), 1853-61.
Enomoto S, et al. Lack of Association Between CpG Island Methylator Phenotype in Human Gastric Cancers and Methylation in Their Background Non-cancerous Gastric Mucosae. Cancer Sci. 2007;98(12):1853-61. PubMed PMID: 17900260.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Lack of association between CpG island methylator phenotype in human gastric cancers and methylation in their background non-cancerous gastric mucosae. AU - Enomoto,Shotaro, AU - Maekita,Takao, AU - Tsukamoto,Tetsuya, AU - Nakajima,Takeshi, AU - Nakazawa,Kazuyuki, AU - Tatematsu,Masae, AU - Ichinose,Masao, AU - Ushijima,Toshikazu, Y1 - 2007/09/26/ PY - 2007/9/29/pubmed PY - 2007/12/22/medline PY - 2007/9/29/entrez SP - 1853 EP - 61 JF - Cancer science JO - Cancer Sci VL - 98 IS - 12 N2 - The presence of high levels of aberrant DNA methylation in gastric mucosae correlates with risk of gastric cancer. Some gastric cancers are known to have methylation of multiple CpG islands (CGI), which is referred to as the CGI methylator phenotype (CIMP). In the present study, we aimed to clarify the possible association between the CIMP in cancers and high methylation levels in their background mucosae by accurate quantitative methylation analysis of 14 carefully selected promoter CGI. Methylation levels were measured in 66 cancers and their background mucosae, along with 19 normal mucosae of healthy volunteers. Methylation in cancers was classified as absent (methylation level = 0%) or positive. The number of methylated CGI in a cancer showed a continuous distribution, and cancers were classified as CIMP high (21 cases), CIMP low (30 cases), or CIMP negative (15 cases). CIMP-high gastric cancer patients had significantly better survival rates than CIMP-negative patients. Of the Epstein-Barr virus-positive gastric cancers studied, eight out of nine presented as CIMP high. Methylation in background mucosae showed a unimodal distribution, and was assessed by their degree. The gastric mucosae of cancer patients showed higher levels than normal gastric mucosae of healthy volunteers. Finally, the CIMP-high, CIMP-low, and CIMP-negative statuses in cancers were not associated with methylation levels of individual genes and their means in the background mucosae. These showed that the CIMP statuses in gastric cancers had no association with methylation levels in the background gastric mucosae. SN - 1349-7006 UR - https://www.unboundmedicine.com/medline/citation/17900260/Lack_of_association_between_CpG_island_methylator_phenotype_in_human_gastric_cancers_and_methylation_in_their_background_non_cancerous_gastric_mucosae_ L2 - https://doi.org/10.1111/j.1349-7006.2007.00625.x DB - PRIME DP - Unbound Medicine ER -