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Target gene selectivity of the myogenic basic helix-loop-helix transcription factor myogenin in embryonic muscle.
Dev Biol. 2007 Nov 15; 311(2):650-64.DB

Abstract

The myogenic regulatory factors MyoD and myogenin are crucial for skeletal muscle development. Despite their importance, the mechanisms by which these factors selectively regulate different target genes are unclear. The purpose of the present investigation was to compare embryonic skeletal muscle from myogenin(+/+) and myogenin(-/-) mice to identify genes whose expression was dependent on the presence of myogenin but not MyoD and to determine whether myogenin-binding sites could be found within regulatory regions of myogenin-dependent genes independent of MyoD. We identified a set of 140 muscle-expressed genes whose expression in embryonic tongue muscle of myogenin(-/-) mice was downregulated in the absence of myogenin, but in the presence of MyoD. Myogenin bound within conserved regulatory regions of several of the downregulated genes, but MyoD bound only to a subset of these same regions, suggesting that many downregulated genes were selective targets of myogenin. The regulatory regions activated gene expression in cultured myoblasts and fibroblasts overexpressing myogenin or MyoD, indicating that expression from exogenously introduced DNA could not recapitulate the selectivity for myogenin observed in vivo. The results identify new target genes for myogenin and show that myogenin's target gene selectivity is not based solely on binding site sequences.

Authors+Show Affiliations

Department of Biochemistry and Molecular Biology, Unit 1000, The University of Texas M. D. Anderson Cancer Center, 1515 Holcombe Boulevard, Houston, TX 77030, USA.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

17904117

Citation

Davie, Judith K., et al. "Target Gene Selectivity of the Myogenic Basic Helix-loop-helix Transcription Factor Myogenin in Embryonic Muscle." Developmental Biology, vol. 311, no. 2, 2007, pp. 650-64.
Davie JK, Cho JH, Meadows E, et al. Target gene selectivity of the myogenic basic helix-loop-helix transcription factor myogenin in embryonic muscle. Dev Biol. 2007;311(2):650-64.
Davie, J. K., Cho, J. H., Meadows, E., Flynn, J. M., Knapp, J. R., & Klein, W. H. (2007). Target gene selectivity of the myogenic basic helix-loop-helix transcription factor myogenin in embryonic muscle. Developmental Biology, 311(2), 650-64.
Davie JK, et al. Target Gene Selectivity of the Myogenic Basic Helix-loop-helix Transcription Factor Myogenin in Embryonic Muscle. Dev Biol. 2007 Nov 15;311(2):650-64. PubMed PMID: 17904117.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Target gene selectivity of the myogenic basic helix-loop-helix transcription factor myogenin in embryonic muscle. AU - Davie,Judith K, AU - Cho,Jang-Hyeon, AU - Meadows,Eric, AU - Flynn,Jesse M, AU - Knapp,Jennifer R, AU - Klein,William H, Y1 - 2007/08/16/ PY - 2007/03/13/received PY - 2007/07/04/revised PY - 2007/08/07/accepted PY - 2007/10/2/pubmed PY - 2008/3/25/medline PY - 2007/10/2/entrez SP - 650 EP - 64 JF - Developmental biology JO - Dev Biol VL - 311 IS - 2 N2 - The myogenic regulatory factors MyoD and myogenin are crucial for skeletal muscle development. Despite their importance, the mechanisms by which these factors selectively regulate different target genes are unclear. The purpose of the present investigation was to compare embryonic skeletal muscle from myogenin(+/+) and myogenin(-/-) mice to identify genes whose expression was dependent on the presence of myogenin but not MyoD and to determine whether myogenin-binding sites could be found within regulatory regions of myogenin-dependent genes independent of MyoD. We identified a set of 140 muscle-expressed genes whose expression in embryonic tongue muscle of myogenin(-/-) mice was downregulated in the absence of myogenin, but in the presence of MyoD. Myogenin bound within conserved regulatory regions of several of the downregulated genes, but MyoD bound only to a subset of these same regions, suggesting that many downregulated genes were selective targets of myogenin. The regulatory regions activated gene expression in cultured myoblasts and fibroblasts overexpressing myogenin or MyoD, indicating that expression from exogenously introduced DNA could not recapitulate the selectivity for myogenin observed in vivo. The results identify new target genes for myogenin and show that myogenin's target gene selectivity is not based solely on binding site sequences. SN - 1095-564X UR - https://www.unboundmedicine.com/medline/citation/17904117/Target_gene_selectivity_of_the_myogenic_basic_helix_loop_helix_transcription_factor_myogenin_in_embryonic_muscle_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0012-1606(07)01260-2 DB - PRIME DP - Unbound Medicine ER -