Tags

Type your tag names separated by a space and hit enter

Sperm chromatin structure assay parameters are not related to fertilization rates, embryo quality, and pregnancy rates in in vitro fertilization and intracytoplasmic sperm injection, but might be related to spontaneous abortion rates.
Fertil Steril. 2008 Aug; 90(2):352-9.FS

Abstract

OBJECTIVE

To investigate the relationship between sperm chromatin structure assay (SCSA) parameters, DNA fragmentation index (DFI) and high DNA stainability (HDS), and outcomes of IVF and intracytoplasmic sperm injection (ICSI).

DESIGN

Retrospective review and prospective study.

SETTING

Academic human reproduction laboratory.

PATIENT(S)

Two hundred twenty-three couples undergoing conventional IVF (n = 137) and ICSI (n = 86).

INTERVENTION(S)

Testing with SCSA on a semen aliquot taken from ejaculate used for assisted reproductive technology (ART).

MAIN OUTCOME MEASURE(S)

Conventional semen parameters, DFI, HDS, outcomes of IVF and ICSI.

RESULT(S)

There were no significant differences in IVF and ICSI fertilization rate, good embryo rate, and pregnancy rate (PR) between high, moderate, and low DFI or HDS groups. Men with HDS >15% had significantly higher IVF abortion rates. There was a statistically insignificant trend toward an increased abortion rate in the high DFI (>27%) group. The DFI correlated negatively with sperm motility, and HDS correlated negatively with sperm morphology and concentration.

CONCLUSION(S)

Neither DFI nor HDS scores can provide independent information about embryo quality, fertilization, and PRs for infertility patients undergoing ART. Sperm DNA fragmentation probably affects sperm motility. The relationship between HDS and IVF abortion rates provides preliminary evidence that ICSI may be indicated for men with HDS >15%. The potential adverse effect of sperm DNA damage on the quality of postimplantation embryo and spontaneous abortion should be a concern.

Authors+Show Affiliations

Department of Obstetrics and Gynecology, Mackay Memorial Hospital, Taipei, Taiwan.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Review

Language

eng

PubMed ID

17904130

Citation

Lin, Ming-Huei, et al. "Sperm Chromatin Structure Assay Parameters Are Not Related to Fertilization Rates, Embryo Quality, and Pregnancy Rates in in Vitro Fertilization and Intracytoplasmic Sperm Injection, but Might Be Related to Spontaneous Abortion Rates." Fertility and Sterility, vol. 90, no. 2, 2008, pp. 352-9.
Lin MH, Kuo-Kuang Lee R, Li SH, et al. Sperm chromatin structure assay parameters are not related to fertilization rates, embryo quality, and pregnancy rates in in vitro fertilization and intracytoplasmic sperm injection, but might be related to spontaneous abortion rates. Fertil Steril. 2008;90(2):352-9.
Lin, M. H., Kuo-Kuang Lee, R., Li, S. H., Lu, C. H., Sun, F. J., & Hwu, Y. M. (2008). Sperm chromatin structure assay parameters are not related to fertilization rates, embryo quality, and pregnancy rates in in vitro fertilization and intracytoplasmic sperm injection, but might be related to spontaneous abortion rates. Fertility and Sterility, 90(2), 352-9.
Lin MH, et al. Sperm Chromatin Structure Assay Parameters Are Not Related to Fertilization Rates, Embryo Quality, and Pregnancy Rates in in Vitro Fertilization and Intracytoplasmic Sperm Injection, but Might Be Related to Spontaneous Abortion Rates. Fertil Steril. 2008;90(2):352-9. PubMed PMID: 17904130.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Sperm chromatin structure assay parameters are not related to fertilization rates, embryo quality, and pregnancy rates in in vitro fertilization and intracytoplasmic sperm injection, but might be related to spontaneous abortion rates. AU - Lin,Ming-Huei, AU - Kuo-Kuang Lee,Robert, AU - Li,Sheng-Hsiang, AU - Lu,Chung-Hao, AU - Sun,Fang-Ju, AU - Hwu,Yuh-Ming, Y1 - 2007/09/27/ PY - 2007/01/30/received PY - 2007/06/08/revised PY - 2007/06/11/accepted PY - 2007/10/2/pubmed PY - 2008/8/15/medline PY - 2007/10/2/entrez SP - 352 EP - 9 JF - Fertility and sterility JO - Fertil. Steril. VL - 90 IS - 2 N2 - OBJECTIVE: To investigate the relationship between sperm chromatin structure assay (SCSA) parameters, DNA fragmentation index (DFI) and high DNA stainability (HDS), and outcomes of IVF and intracytoplasmic sperm injection (ICSI). DESIGN: Retrospective review and prospective study. SETTING: Academic human reproduction laboratory. PATIENT(S): Two hundred twenty-three couples undergoing conventional IVF (n = 137) and ICSI (n = 86). INTERVENTION(S): Testing with SCSA on a semen aliquot taken from ejaculate used for assisted reproductive technology (ART). MAIN OUTCOME MEASURE(S): Conventional semen parameters, DFI, HDS, outcomes of IVF and ICSI. RESULT(S): There were no significant differences in IVF and ICSI fertilization rate, good embryo rate, and pregnancy rate (PR) between high, moderate, and low DFI or HDS groups. Men with HDS >15% had significantly higher IVF abortion rates. There was a statistically insignificant trend toward an increased abortion rate in the high DFI (>27%) group. The DFI correlated negatively with sperm motility, and HDS correlated negatively with sperm morphology and concentration. CONCLUSION(S): Neither DFI nor HDS scores can provide independent information about embryo quality, fertilization, and PRs for infertility patients undergoing ART. Sperm DNA fragmentation probably affects sperm motility. The relationship between HDS and IVF abortion rates provides preliminary evidence that ICSI may be indicated for men with HDS >15%. The potential adverse effect of sperm DNA damage on the quality of postimplantation embryo and spontaneous abortion should be a concern. SN - 1556-5653 UR - https://www.unboundmedicine.com/medline/citation/17904130/Sperm_chromatin_structure_assay_parameters_are_not_related_to_fertilization_rates_embryo_quality_and_pregnancy_rates_in_in_vitro_fertilization_and_intracytoplasmic_sperm_injection_but_might_be_related_to_spontaneous_abortion_rates_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0015-0282(07)01361-1 DB - PRIME DP - Unbound Medicine ER -