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Anti-apoptotic effect of esculin on dopamine-induced cytotoxicity in the human neuroblastoma SH-SY5Y cell line.
Neuropharmacology. 2007 Nov; 53(6):724-32.N

Abstract

Dopamine (DA), as a neurotoxin, can elicit severe Parkinson's disease-like syndrome by elevating intracellular reactive oxygen species (ROS) levels and apoptotic activity. In this study, we examined the effect of esculin, which was extracted from Fraxinus sielboldiana blume, on DA-induced cytotoxicity and the underlying mechanism in human neuroblastoma SH-SY5Y cells. Our results suggest that the protective effects of esculin (10(-7), 10(-6) and 10(-5) M) on DA-induced cytotoxicity may be ascribed to its anti-oxidative properties by reducing ROS level, and its anti-apoptotic effect via protecting mitochondrion membrane potential (DeltaPsim), enhancing superoxide dismutaese (SOD) activity and reduced glutathione (GSH) levels, and regulating P53, Bax and Bcl-2 expression. In addition, esculin inhibited the release of cytochrome c and apoptosis-inducing factor (AIF), and the protein expression of activated caspase 3. These data indicate that esculin may provide a useful therapeutic strategy for the treatment of progressive neurodegenerative diseases such as Parkinson's disease (PD).

Authors+Show Affiliations

Key Laboratory of Bioactive Substances and Resources Utilization of Chinese Herbal Medicine affiliated Ministry of Education, Institute of Materia Medica, Chinese Academy of Medical Sciences & Peking Union Medical College, District Xuanwu, Beijing, China.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

17904593

Citation

Zhao, Da-Long, et al. "Anti-apoptotic Effect of Esculin On Dopamine-induced Cytotoxicity in the Human Neuroblastoma SH-SY5Y Cell Line." Neuropharmacology, vol. 53, no. 6, 2007, pp. 724-32.
Zhao DL, Zou LB, Lin S, et al. Anti-apoptotic effect of esculin on dopamine-induced cytotoxicity in the human neuroblastoma SH-SY5Y cell line. Neuropharmacology. 2007;53(6):724-32.
Zhao, D. L., Zou, L. B., Lin, S., Shi, J. G., & Zhu, H. B. (2007). Anti-apoptotic effect of esculin on dopamine-induced cytotoxicity in the human neuroblastoma SH-SY5Y cell line. Neuropharmacology, 53(6), 724-32.
Zhao DL, et al. Anti-apoptotic Effect of Esculin On Dopamine-induced Cytotoxicity in the Human Neuroblastoma SH-SY5Y Cell Line. Neuropharmacology. 2007;53(6):724-32. PubMed PMID: 17904593.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Anti-apoptotic effect of esculin on dopamine-induced cytotoxicity in the human neuroblastoma SH-SY5Y cell line. AU - Zhao,Da-Long, AU - Zou,Li-Bo, AU - Lin,Sheng, AU - Shi,Jian-Gong, AU - Zhu,Hai-Bo, Y1 - 2007/08/16/ PY - 2007/02/13/received PY - 2007/07/31/revised PY - 2007/07/31/accepted PY - 2007/10/2/pubmed PY - 2007/12/22/medline PY - 2007/10/2/entrez SP - 724 EP - 32 JF - Neuropharmacology JO - Neuropharmacology VL - 53 IS - 6 N2 - Dopamine (DA), as a neurotoxin, can elicit severe Parkinson's disease-like syndrome by elevating intracellular reactive oxygen species (ROS) levels and apoptotic activity. In this study, we examined the effect of esculin, which was extracted from Fraxinus sielboldiana blume, on DA-induced cytotoxicity and the underlying mechanism in human neuroblastoma SH-SY5Y cells. Our results suggest that the protective effects of esculin (10(-7), 10(-6) and 10(-5) M) on DA-induced cytotoxicity may be ascribed to its anti-oxidative properties by reducing ROS level, and its anti-apoptotic effect via protecting mitochondrion membrane potential (DeltaPsim), enhancing superoxide dismutaese (SOD) activity and reduced glutathione (GSH) levels, and regulating P53, Bax and Bcl-2 expression. In addition, esculin inhibited the release of cytochrome c and apoptosis-inducing factor (AIF), and the protein expression of activated caspase 3. These data indicate that esculin may provide a useful therapeutic strategy for the treatment of progressive neurodegenerative diseases such as Parkinson's disease (PD). SN - 0028-3908 UR - https://www.unboundmedicine.com/medline/citation/17904593/Anti_apoptotic_effect_of_esculin_on_dopamine_induced_cytotoxicity_in_the_human_neuroblastoma_SH_SY5Y_cell_line_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0028-3908(07)00245-6 DB - PRIME DP - Unbound Medicine ER -