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Investigating the relationship between embryotoxic and genotoxic effects of benzo[a]pyrene, 17alpha-ethinylestradiol and endosulfan on Crassostrea gigas embryos.
Aquat Toxicol. 2007 Nov 30; 85(2):133-42.AT

Abstract

Genotoxicity biomarkers are widely measured in ecotoxicology as molecular toxic endpoints of major environmental pollutants. However, the long-term consequences of such damage still have to be elucidated. Some authors have suggested that the accumulation of unrepaired DNA lesions could explain the embryotoxicity of certain chemical pollutants. As embryotoxicity exerts a direct impact on the recruitment rate, genotoxicity could be closely related to disturbances of ecological concern and produce a possible impact upon population dynamics. The aim of the present work was to study the genotoxicity and the embryotoxicity of three relevant pollutants for oyster embryos: the polycyclic aromatic hydrocarbon, benzo[a]pyrene (BaP), the synthetic estrogenic hormone, 17alpha-ethinylestradiol (EE2), and the organochlorine pesticide, endosulfan (ES). For each substance, gamete fertilization was performed and embryo development followed in contaminated reference seawater. Following exposure, embryotoxicity was evaluated by calculating the percentage of abnormal D-larvae obtained at 20 h development. Genotoxicity was measured in parallel by conducting a comet assay on enzymatically dissociated cells of pre-shelled larvae (16 h development). The oxidized DNA base, 8-oxodGuo, was also measured by HPLC coupled to electrochemical detection. For each contaminant, the relationship between genotoxicity and embryotoxicity was then studied to check for the possible significance of genotoxicity in the population dynamics of marine bivalves from polluted areas. For BaP, embryotoxicity and DNA strand breakage were both observed from the lowest tested concentration of 0.2 nM. Induction of 8-oxodGuo was significant from 20 nM. Endosulfan exposure resulted in similar effects for oyster embryos but from higher concentrations and followed a concentration-dependent manner. Embryotoxicity and genotoxicity in terms of DNA strand breaks were observed for endosulfan from 300 and 150 nM, respectively. No change in 8-oxodGuo level was observed following endosulfan exposure. EE2 displayed no toxic effect for oyster embryos within the range of tested concentrations (from 0.02 to 1.7 nM). Taking into account all the data collected during this study, a positive and significant correlation was demonstrated in oyster embryos between genotoxicity as measured by the comet assay and embryotoxicity.

Authors+Show Affiliations

Ifremer, Département de Biogéochimie et Ecotoxicologie, Rue de l'Ile d'Yeu, 44311 Nantes Cedex 03, France.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Journal Article

Language

eng

PubMed ID

17904659

Citation

Wessel, N, et al. "Investigating the Relationship Between Embryotoxic and Genotoxic Effects of Benzo[a]pyrene, 17alpha-ethinylestradiol and Endosulfan On Crassostrea Gigas Embryos." Aquatic Toxicology (Amsterdam, Netherlands), vol. 85, no. 2, 2007, pp. 133-42.
Wessel N, Rousseau S, Caisey X, et al. Investigating the relationship between embryotoxic and genotoxic effects of benzo[a]pyrene, 17alpha-ethinylestradiol and endosulfan on Crassostrea gigas embryos. Aquat Toxicol. 2007;85(2):133-42.
Wessel, N., Rousseau, S., Caisey, X., Quiniou, F., & Akcha, F. (2007). Investigating the relationship between embryotoxic and genotoxic effects of benzo[a]pyrene, 17alpha-ethinylestradiol and endosulfan on Crassostrea gigas embryos. Aquatic Toxicology (Amsterdam, Netherlands), 85(2), 133-42.
Wessel N, et al. Investigating the Relationship Between Embryotoxic and Genotoxic Effects of Benzo[a]pyrene, 17alpha-ethinylestradiol and Endosulfan On Crassostrea Gigas Embryos. Aquat Toxicol. 2007 Nov 30;85(2):133-42. PubMed PMID: 17904659.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Investigating the relationship between embryotoxic and genotoxic effects of benzo[a]pyrene, 17alpha-ethinylestradiol and endosulfan on Crassostrea gigas embryos. AU - Wessel,N, AU - Rousseau,S, AU - Caisey,X, AU - Quiniou,F, AU - Akcha,F, Y1 - 2007/08/24/ PY - 2007/07/13/received PY - 2007/08/16/accepted PY - 2007/10/2/pubmed PY - 2007/12/14/medline PY - 2007/10/2/entrez SP - 133 EP - 42 JF - Aquatic toxicology (Amsterdam, Netherlands) JO - Aquat Toxicol VL - 85 IS - 2 N2 - Genotoxicity biomarkers are widely measured in ecotoxicology as molecular toxic endpoints of major environmental pollutants. However, the long-term consequences of such damage still have to be elucidated. Some authors have suggested that the accumulation of unrepaired DNA lesions could explain the embryotoxicity of certain chemical pollutants. As embryotoxicity exerts a direct impact on the recruitment rate, genotoxicity could be closely related to disturbances of ecological concern and produce a possible impact upon population dynamics. The aim of the present work was to study the genotoxicity and the embryotoxicity of three relevant pollutants for oyster embryos: the polycyclic aromatic hydrocarbon, benzo[a]pyrene (BaP), the synthetic estrogenic hormone, 17alpha-ethinylestradiol (EE2), and the organochlorine pesticide, endosulfan (ES). For each substance, gamete fertilization was performed and embryo development followed in contaminated reference seawater. Following exposure, embryotoxicity was evaluated by calculating the percentage of abnormal D-larvae obtained at 20 h development. Genotoxicity was measured in parallel by conducting a comet assay on enzymatically dissociated cells of pre-shelled larvae (16 h development). The oxidized DNA base, 8-oxodGuo, was also measured by HPLC coupled to electrochemical detection. For each contaminant, the relationship between genotoxicity and embryotoxicity was then studied to check for the possible significance of genotoxicity in the population dynamics of marine bivalves from polluted areas. For BaP, embryotoxicity and DNA strand breakage were both observed from the lowest tested concentration of 0.2 nM. Induction of 8-oxodGuo was significant from 20 nM. Endosulfan exposure resulted in similar effects for oyster embryos but from higher concentrations and followed a concentration-dependent manner. Embryotoxicity and genotoxicity in terms of DNA strand breaks were observed for endosulfan from 300 and 150 nM, respectively. No change in 8-oxodGuo level was observed following endosulfan exposure. EE2 displayed no toxic effect for oyster embryos within the range of tested concentrations (from 0.02 to 1.7 nM). Taking into account all the data collected during this study, a positive and significant correlation was demonstrated in oyster embryos between genotoxicity as measured by the comet assay and embryotoxicity. SN - 0166-445X UR - https://www.unboundmedicine.com/medline/citation/17904659/Investigating_the_relationship_between_embryotoxic_and_genotoxic_effects_of_benzo[a]pyrene_17alpha_ethinylestradiol_and_endosulfan_on_Crassostrea_gigas_embryos_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0166-445X(07)00327-X DB - PRIME DP - Unbound Medicine ER -