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[Effect of electroacupuncture on carrageenan-induced inflammation, IL-1beta and TNF-alpha concentrations and their mRNA expressions in toe tissue in rats].
Zhen Ci Yan Jiu 2007; 32(4):224-8ZC

Abstract

OBJECTIVE

To observe the anti-inflammatory effects of electroacupuncture (EA) at "Quchi" (LI 11) and its influence on IL-1beta, TNF-alpha concentrations and mRNA expressions in carrageenan (CAR)-induced inflammation rats, so as to reveal partial mechanisms of EA in relieving acute inflammation.

METHODS

Sixty male Wistar rats were divided into control, model, Indomethacin (IND, 7.5 mg/kg, oral perfusion), Rofecoxib (7.5 mg/kg, oral perfusion), EA-pre-treatment (EA was given once daily for 5 days before modeling), EA-post-treatment (EA was given once after modeling) groups, with 10 cases in each. EA (2 Hz, 5 mA and persistent waves) was applied to "Quchi" (LI 11) for 30 min each time. Arthritis model was established by subcutaneous injection of 1% CAR, 0.1 mL into the right hind paw. Paw edema degree of the affected hind paw was measured with a foot-volume surveyor. After processing samples of the affected paw toes (including smashing, centrifugalization, etc.), concentrations of prostaglandin (PG) E2, interleukin (IL)-1beta, tumor necrosis factor (TNF)-alpha were measured by enzyme linked immunosorbent assay (ELISA). Expressions of IL-1beta mRNA and TNF-alpha mRNA in the local inflammatory tissues were detected by reverse-transcription polymerase chain reaction (RT-PCR) assay.

RESULTS

Compared with model group, the affected hind-paw-volume decreased significantly from 2 h on in EA-post-treatment group and from 1 h on in IND group after injection of CAR. Compared with control group, PGE2, IL-1beta and TNF-alpha contents in the toe tissue of model group increased significantly; while in comparison with model group, PGE2 and IL-1beta levels in EA-post-treatment group, IL-1beta level in IND group decreased significantly (P < 0.05), and TNF-alpha level in IND group increased significantly. No significant differences were found between model group and Rofecoxib and EA-pre-treatment groups in most time-courses of the hind-paw-volume, between model and IND, Rofecoxib and EA-pre-treatment groups in PGE2, between model and EA-pre-treatment groups in IL-1beta, and between model and Rofecoxib, EA-pre-treatment and EA-post-treatment groups in TNF-alpha contents (P > 0.05). Compared with model group, IL-1beta mRNA expression of the local inflammatory tissues in IND and EA-post-treatment groups was down-regulated, and TNF-alpha mRNA expression in Rofecoxib and two EA groups down-regulated slightly.

CONCLUSION

EA possesses anti-inflammatory effect in acute arthritis rats, which is closely related to its effects in reducing the secretion of PGE2, IL-1beta and TNF-alpha and the expression of IL-1beta mRNA and TNF-alpha mRNA.

Authors+Show Affiliations

The Third Clinical Medical School, Zhejiang Chinese Medical University, Hangzhou 310053, China. fangjianqiao@yahoo.comNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

English Abstract
Journal Article
Research Support, Non-U.S. Gov't

Language

chi

PubMed ID

17907382

Citation

Fang, Jian-qiao, et al. "[Effect of Electroacupuncture On Carrageenan-induced Inflammation, IL-1beta and TNF-alpha Concentrations and Their mRNA Expressions in Toe Tissue in Rats]." Zhen Ci Yan Jiu = Acupuncture Research, vol. 32, no. 4, 2007, pp. 224-8.
Fang JQ, Liu F, Shao XM, et al. [Effect of electroacupuncture on carrageenan-induced inflammation, IL-1beta and TNF-alpha concentrations and their mRNA expressions in toe tissue in rats]. Zhen Ci Yan Jiu. 2007;32(4):224-8.
Fang, J. Q., Liu, F., Shao, X. M., & Wu, Y. Y. (2007). [Effect of electroacupuncture on carrageenan-induced inflammation, IL-1beta and TNF-alpha concentrations and their mRNA expressions in toe tissue in rats]. Zhen Ci Yan Jiu = Acupuncture Research, 32(4), pp. 224-8.
Fang JQ, et al. [Effect of Electroacupuncture On Carrageenan-induced Inflammation, IL-1beta and TNF-alpha Concentrations and Their mRNA Expressions in Toe Tissue in Rats]. Zhen Ci Yan Jiu. 2007;32(4):224-8. PubMed PMID: 17907382.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - [Effect of electroacupuncture on carrageenan-induced inflammation, IL-1beta and TNF-alpha concentrations and their mRNA expressions in toe tissue in rats]. AU - Fang,Jian-qiao, AU - Liu,Fang, AU - Shao,Xiao-mei, AU - Wu,Yuan-yuan, PY - 2007/10/3/pubmed PY - 2007/10/18/medline PY - 2007/10/3/entrez SP - 224 EP - 8 JF - Zhen ci yan jiu = Acupuncture research JO - Zhen Ci Yan Jiu VL - 32 IS - 4 N2 - OBJECTIVE: To observe the anti-inflammatory effects of electroacupuncture (EA) at "Quchi" (LI 11) and its influence on IL-1beta, TNF-alpha concentrations and mRNA expressions in carrageenan (CAR)-induced inflammation rats, so as to reveal partial mechanisms of EA in relieving acute inflammation. METHODS: Sixty male Wistar rats were divided into control, model, Indomethacin (IND, 7.5 mg/kg, oral perfusion), Rofecoxib (7.5 mg/kg, oral perfusion), EA-pre-treatment (EA was given once daily for 5 days before modeling), EA-post-treatment (EA was given once after modeling) groups, with 10 cases in each. EA (2 Hz, 5 mA and persistent waves) was applied to "Quchi" (LI 11) for 30 min each time. Arthritis model was established by subcutaneous injection of 1% CAR, 0.1 mL into the right hind paw. Paw edema degree of the affected hind paw was measured with a foot-volume surveyor. After processing samples of the affected paw toes (including smashing, centrifugalization, etc.), concentrations of prostaglandin (PG) E2, interleukin (IL)-1beta, tumor necrosis factor (TNF)-alpha were measured by enzyme linked immunosorbent assay (ELISA). Expressions of IL-1beta mRNA and TNF-alpha mRNA in the local inflammatory tissues were detected by reverse-transcription polymerase chain reaction (RT-PCR) assay. RESULTS: Compared with model group, the affected hind-paw-volume decreased significantly from 2 h on in EA-post-treatment group and from 1 h on in IND group after injection of CAR. Compared with control group, PGE2, IL-1beta and TNF-alpha contents in the toe tissue of model group increased significantly; while in comparison with model group, PGE2 and IL-1beta levels in EA-post-treatment group, IL-1beta level in IND group decreased significantly (P < 0.05), and TNF-alpha level in IND group increased significantly. No significant differences were found between model group and Rofecoxib and EA-pre-treatment groups in most time-courses of the hind-paw-volume, between model and IND, Rofecoxib and EA-pre-treatment groups in PGE2, between model and EA-pre-treatment groups in IL-1beta, and between model and Rofecoxib, EA-pre-treatment and EA-post-treatment groups in TNF-alpha contents (P > 0.05). Compared with model group, IL-1beta mRNA expression of the local inflammatory tissues in IND and EA-post-treatment groups was down-regulated, and TNF-alpha mRNA expression in Rofecoxib and two EA groups down-regulated slightly. CONCLUSION: EA possesses anti-inflammatory effect in acute arthritis rats, which is closely related to its effects in reducing the secretion of PGE2, IL-1beta and TNF-alpha and the expression of IL-1beta mRNA and TNF-alpha mRNA. SN - 1000-0607 UR - https://www.unboundmedicine.com/medline/citation/17907382/[Effect_of_electroacupuncture_on_carrageenan_induced_inflammation_IL_1beta_and_TNF_alpha_concentrations_and_their_mRNA_expressions_in_toe_tissue_in_rats]_ DB - PRIME DP - Unbound Medicine ER -