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Involvement of cannabinoid receptor CB2 in dectin-1-mediated macrophage phagocytosis.
Immunol Cell Biol 2008; 86(2):179-84IC

Abstract

Cannabinoid receptors are expressed in macrophages, but little is known of their roles. We here examined their involvement in phagocytosis. The presence of 2-arachidonylglycerol, an endocannabinoid, augmented the phagocytosis of zymosan by mouse macrophages, while the phagocytosis of Escherichia coli, Staphylococcus aureus, apoptotic cells or latex beads remained unaffected. An agonist of the cannabinoid receptors CB1 and CB2 also stimulated the phagocytosis of zymosan. The stimulatory effect of 2-arachidonylglycerol was abolished when phagocytosis reactions were carried out in the presence of an antagonist of CB2 but not of CB1. Furthermore, the phagocytosis of zymosan in the presence of 2-arachidonylglycerol was severely inhibited by the addition of a beta-glucan-containing carbohydrate or antibody neutralizing dectin-1, a beta-glucan-recognizing phagocytosis receptor. These results suggested that the activation of CB2 in macrophages leads to the stimulation of dectin-1-mediated phagocytosis.

Authors+Show Affiliations

Graduate School of Medical Science, Kanazawa University, Kanazawa, Japan.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

17909562

Citation

Shiratsuchi, Akiko, et al. "Involvement of Cannabinoid Receptor CB2 in Dectin-1-mediated Macrophage Phagocytosis." Immunology and Cell Biology, vol. 86, no. 2, 2008, pp. 179-84.
Shiratsuchi A, Watanabe I, Yoshida H, et al. Involvement of cannabinoid receptor CB2 in dectin-1-mediated macrophage phagocytosis. Immunol Cell Biol. 2008;86(2):179-84.
Shiratsuchi, A., Watanabe, I., Yoshida, H., & Nakanishi, Y. (2008). Involvement of cannabinoid receptor CB2 in dectin-1-mediated macrophage phagocytosis. Immunology and Cell Biology, 86(2), pp. 179-84.
Shiratsuchi A, et al. Involvement of Cannabinoid Receptor CB2 in Dectin-1-mediated Macrophage Phagocytosis. Immunol Cell Biol. 2008;86(2):179-84. PubMed PMID: 17909562.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Involvement of cannabinoid receptor CB2 in dectin-1-mediated macrophage phagocytosis. AU - Shiratsuchi,Akiko, AU - Watanabe,Ikuko, AU - Yoshida,Hiroko, AU - Nakanishi,Yoshinobu, Y1 - 2007/10/02/ PY - 2007/10/3/pubmed PY - 2008/5/23/medline PY - 2007/10/3/entrez SP - 179 EP - 84 JF - Immunology and cell biology JO - Immunol. Cell Biol. VL - 86 IS - 2 N2 - Cannabinoid receptors are expressed in macrophages, but little is known of their roles. We here examined their involvement in phagocytosis. The presence of 2-arachidonylglycerol, an endocannabinoid, augmented the phagocytosis of zymosan by mouse macrophages, while the phagocytosis of Escherichia coli, Staphylococcus aureus, apoptotic cells or latex beads remained unaffected. An agonist of the cannabinoid receptors CB1 and CB2 also stimulated the phagocytosis of zymosan. The stimulatory effect of 2-arachidonylglycerol was abolished when phagocytosis reactions were carried out in the presence of an antagonist of CB2 but not of CB1. Furthermore, the phagocytosis of zymosan in the presence of 2-arachidonylglycerol was severely inhibited by the addition of a beta-glucan-containing carbohydrate or antibody neutralizing dectin-1, a beta-glucan-recognizing phagocytosis receptor. These results suggested that the activation of CB2 in macrophages leads to the stimulation of dectin-1-mediated phagocytosis. SN - 0818-9641 UR - https://www.unboundmedicine.com/medline/citation/17909562/Involvement_of_cannabinoid_receptor_CB2_in_dectin_1_mediated_macrophage_phagocytosis_ L2 - https://doi.org/10.1038/sj.icb.7100121 DB - PRIME DP - Unbound Medicine ER -