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Fast gas chromatography and negative-ion chemical ionization tandem mass spectrometry for forensic analysis of cannabinoids in whole blood.
J Pharm Biomed Anal. 2007 Nov 05; 45(3):495-503.JP

Abstract

The present work describes a fast gas chromatography/negative-ion chemical ionization tandem mass spectrometric assay (Fast GC/NICI-MS/MS) for analysis of tetrahydrocannabinol (THC), 11-hydroxy-tetrahydrocannabinol (THC-OH) and 11-nor-9-carboxy-tetrahydrocannabinol (THC-COOH) in whole blood. The cannabinoids were extracted from 500 microL of whole blood by a simple liquid-liquid extraction (LLE) and then derivatized by using trifluoroacetic anhydride (TFAA) and hexafluoro-2-propanol (HFIP) as fluorinated agents. Mass spectrometric detection of the analytes was performed in the selected reaction-monitoring mode on a triple quadrupole instrument after negative-ion chemical ionization. The assay was found to be linear in the concentration range of 0.5-20 ng/mL for THC and THC-OH, and of 2.5-100 ng/mL for THC-COOH. Repeatability and intermediate precision were found less than 12% for all concentrations tested. Under standard chromatographic conditions, the run cycle time would have been 15 min. By using fast conditions of separation, the assay analysis time has been reduced to 5 min, without compromising the chromatographic resolution. Finally, a simple approach for estimating the uncertainty measurement is presented.

Authors+Show Affiliations

Institute of Forensic Medicine, University of Geneva, Avenue de Champel 9, 1211 Geneva 4, Switzerland.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

17913432

Citation

Thomas, Aurélien, et al. "Fast Gas Chromatography and Negative-ion Chemical Ionization Tandem Mass Spectrometry for Forensic Analysis of Cannabinoids in Whole Blood." Journal of Pharmaceutical and Biomedical Analysis, vol. 45, no. 3, 2007, pp. 495-503.
Thomas A, Widmer C, Hopfgartner G, et al. Fast gas chromatography and negative-ion chemical ionization tandem mass spectrometry for forensic analysis of cannabinoids in whole blood. J Pharm Biomed Anal. 2007;45(3):495-503.
Thomas, A., Widmer, C., Hopfgartner, G., & Staub, C. (2007). Fast gas chromatography and negative-ion chemical ionization tandem mass spectrometry for forensic analysis of cannabinoids in whole blood. Journal of Pharmaceutical and Biomedical Analysis, 45(3), 495-503.
Thomas A, et al. Fast Gas Chromatography and Negative-ion Chemical Ionization Tandem Mass Spectrometry for Forensic Analysis of Cannabinoids in Whole Blood. J Pharm Biomed Anal. 2007 Nov 5;45(3):495-503. PubMed PMID: 17913432.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Fast gas chromatography and negative-ion chemical ionization tandem mass spectrometry for forensic analysis of cannabinoids in whole blood. AU - Thomas,Aurélien, AU - Widmer,Christèle, AU - Hopfgartner,Gérard, AU - Staub,Christian, Y1 - 2007/08/23/ PY - 2007/05/04/received PY - 2007/08/13/revised PY - 2007/08/16/accepted PY - 2007/10/5/pubmed PY - 2008/3/4/medline PY - 2007/10/5/entrez SP - 495 EP - 503 JF - Journal of pharmaceutical and biomedical analysis JO - J Pharm Biomed Anal VL - 45 IS - 3 N2 - The present work describes a fast gas chromatography/negative-ion chemical ionization tandem mass spectrometric assay (Fast GC/NICI-MS/MS) for analysis of tetrahydrocannabinol (THC), 11-hydroxy-tetrahydrocannabinol (THC-OH) and 11-nor-9-carboxy-tetrahydrocannabinol (THC-COOH) in whole blood. The cannabinoids were extracted from 500 microL of whole blood by a simple liquid-liquid extraction (LLE) and then derivatized by using trifluoroacetic anhydride (TFAA) and hexafluoro-2-propanol (HFIP) as fluorinated agents. Mass spectrometric detection of the analytes was performed in the selected reaction-monitoring mode on a triple quadrupole instrument after negative-ion chemical ionization. The assay was found to be linear in the concentration range of 0.5-20 ng/mL for THC and THC-OH, and of 2.5-100 ng/mL for THC-COOH. Repeatability and intermediate precision were found less than 12% for all concentrations tested. Under standard chromatographic conditions, the run cycle time would have been 15 min. By using fast conditions of separation, the assay analysis time has been reduced to 5 min, without compromising the chromatographic resolution. Finally, a simple approach for estimating the uncertainty measurement is presented. SN - 0731-7085 UR - https://www.unboundmedicine.com/medline/citation/17913432/Fast_gas_chromatography_and_negative_ion_chemical_ionization_tandem_mass_spectrometry_for_forensic_analysis_of_cannabinoids_in_whole_blood_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0731-7085(07)00479-7 DB - PRIME DP - Unbound Medicine ER -