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High diversity of extended-spectrum beta-lactamases among clinical isolates of Enterobacteriaceae from Portugal.
J Antimicrob Chemother. 2007 Dec; 60(6):1370-4.JA

Abstract

OBJECTIVES

To investigate the occurrence and the diversity of Ambler class A ESBLs among Enterobacteriaceae from different Portuguese clinical settings over a 2 year period (2002-04).

METHODS

One hundred and nine extended-spectrum beta-lactamase (ESBL)-producing Enterobacteriaceae isolates from five geographically distant health institutions in Portugal were studied. ESBLs were characterized by isoelectric focussing, PCR and further sequencing. Antibiotic susceptibility testing, transfer of resistance genes and clonal diversity were determined by standard procedures. Plasmid relatedness was established by comparison of random amplified polymorphic DNA (RAPD) patterns.

RESULTS

ESBLs were identified as TEM (46%), SHV (30%), CTX-M (22%) and GES (2%) types; TEM-24, TEM-52, SHV-12 and CTX-M-15 enzymes being the most frequently found. Inter-hospital dissemination of epidemic strains harbouring the most prevalent ESBLs was detected, including the TEM-24-producing Enterobacter aerogenes European epidemic clone. Conjugative transfer of ESBLs was achieved for 67% of isolates and epidemic plasmids containing specific bla genes were detected (bla(CTX-M-15) and bla(TEM-24)). We describe two new ESBLs, SHV-90 (A187T, G238S and E240K) and SHV-91 (P20S and E240K), and a new TEM-type enzyme conferring a phenotype resembling that of a complex mutant TEM beta-lactamase, designated as TEM-154 (M69L and R164S). The broad-spectrum beta-lactamases SHV-26, SHV-36 and TEM-110 were first observed in our country.

CONCLUSIONS

We describe a complex ESBL epidemiology in Portugal, including widespread dissemination of known strains and plasmids coding for TEM-24 and CTX-M-15 enzymes as observed in other European countries.

Authors+Show Affiliations

REQUIMTE, Faculdade de Farmácia, Universidade do Porto, Porto, Portugal.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

17913717

Citation

Machado, Elisabete, et al. "High Diversity of Extended-spectrum Beta-lactamases Among Clinical Isolates of Enterobacteriaceae From Portugal." The Journal of Antimicrobial Chemotherapy, vol. 60, no. 6, 2007, pp. 1370-4.
Machado E, Coque TM, Cantón R, et al. High diversity of extended-spectrum beta-lactamases among clinical isolates of Enterobacteriaceae from Portugal. J Antimicrob Chemother. 2007;60(6):1370-4.
Machado, E., Coque, T. M., Cantón, R., Novais, A., Sousa, J. C., Baquero, F., & Peixe, L. (2007). High diversity of extended-spectrum beta-lactamases among clinical isolates of Enterobacteriaceae from Portugal. The Journal of Antimicrobial Chemotherapy, 60(6), 1370-4.
Machado E, et al. High Diversity of Extended-spectrum Beta-lactamases Among Clinical Isolates of Enterobacteriaceae From Portugal. J Antimicrob Chemother. 2007;60(6):1370-4. PubMed PMID: 17913717.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - High diversity of extended-spectrum beta-lactamases among clinical isolates of Enterobacteriaceae from Portugal. AU - Machado,Elisabete, AU - Coque,Teresa M, AU - Cantón,Rafael, AU - Novais,Angela, AU - Sousa,João Carlos, AU - Baquero,Fernando, AU - Peixe,Luísa, AU - ,, Y1 - 2007/10/03/ PY - 2007/10/5/pubmed PY - 2008/2/27/medline PY - 2007/10/5/entrez SP - 1370 EP - 4 JF - The Journal of antimicrobial chemotherapy JO - J. Antimicrob. Chemother. VL - 60 IS - 6 N2 - OBJECTIVES: To investigate the occurrence and the diversity of Ambler class A ESBLs among Enterobacteriaceae from different Portuguese clinical settings over a 2 year period (2002-04). METHODS: One hundred and nine extended-spectrum beta-lactamase (ESBL)-producing Enterobacteriaceae isolates from five geographically distant health institutions in Portugal were studied. ESBLs were characterized by isoelectric focussing, PCR and further sequencing. Antibiotic susceptibility testing, transfer of resistance genes and clonal diversity were determined by standard procedures. Plasmid relatedness was established by comparison of random amplified polymorphic DNA (RAPD) patterns. RESULTS: ESBLs were identified as TEM (46%), SHV (30%), CTX-M (22%) and GES (2%) types; TEM-24, TEM-52, SHV-12 and CTX-M-15 enzymes being the most frequently found. Inter-hospital dissemination of epidemic strains harbouring the most prevalent ESBLs was detected, including the TEM-24-producing Enterobacter aerogenes European epidemic clone. Conjugative transfer of ESBLs was achieved for 67% of isolates and epidemic plasmids containing specific bla genes were detected (bla(CTX-M-15) and bla(TEM-24)). We describe two new ESBLs, SHV-90 (A187T, G238S and E240K) and SHV-91 (P20S and E240K), and a new TEM-type enzyme conferring a phenotype resembling that of a complex mutant TEM beta-lactamase, designated as TEM-154 (M69L and R164S). The broad-spectrum beta-lactamases SHV-26, SHV-36 and TEM-110 were first observed in our country. CONCLUSIONS: We describe a complex ESBL epidemiology in Portugal, including widespread dissemination of known strains and plasmids coding for TEM-24 and CTX-M-15 enzymes as observed in other European countries. SN - 0305-7453 UR - https://www.unboundmedicine.com/medline/citation/17913717/High_diversity_of_extended_spectrum_beta_lactamases_among_clinical_isolates_of_Enterobacteriaceae_from_Portugal_ L2 - https://academic.oup.com/jac/article-lookup/doi/10.1093/jac/dkm381 DB - PRIME DP - Unbound Medicine ER -