Tags

Type your tag names separated by a space and hit enter

Development and evaluation of an Influenza virus subtype H7N2 vaccine candidate for pandemic preparedness.
Clin Vaccine Immunol. 2007 Nov; 14(11):1425-32.CV

Abstract

Influenza virus of the H7N2 subtype has been introduced into noncommercial poultry in the United States, and this probably resulted in incidents of transmission of H7N2 virus to humans, documented in 2002 and 2003. This virus could be considered a potential threat to public health if it acquired person-to-person transmissibility. A favored approach for global pandemic preparedness includes development of prepandemic vaccines for any potential pandemic virus. To this end, we created a high-growth reassortant virus (H7N2-PR8) containing the genes for the hemagglutinin and the neuraminidase from a low-pathogenicity (H7N2) virus strain and the remaining six genes from a human vaccine strain (H1N1). The reassortant strain was evaluated to assess its antigenicity, safety, and protective efficacy using a mouse model. Antigenicity studies using ferret antibodies raised against H7N2-PR8 indicated that this virus confers broad cross-reactivity with divergent H7 viruses of different years and lineages. Mice and chickens inoculated with high doses of H7N2-PR8 supported virus replication but survived, indicating that this virus is comparable to other avian viruses of low pathogenicity. To assess the protective efficacy of H7N2-PR8, mice were immunized with two doses of formalin-inactivated H7N2-PR8, alone or with alum. Vaccinated mice subsequently challenged with highly pathogenic viruses from homologous and heterologous lineages A/Canada/444/04 (H7N3) and A/Netherlands/219/03 (H7N7) showed pronounced reduction of wild-type virus replication. These studies indicate that H7N2-PR8 is immunogenic, safe, and protective in animal models; these are the essential attributes to qualify for phase I human clinical trials as a prepandemic vaccine.

Authors+Show Affiliations

Influenza Division, Mailstop G-16, Centers for Disease Control and Prevention, 1600 Clifton Road, Atlanta, GA 30333, USA.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, U.S. Gov't, Non-P.H.S.

Language

eng

PubMed ID

17913860

Citation

Pappas, Claudia, et al. "Development and Evaluation of an Influenza Virus Subtype H7N2 Vaccine Candidate for Pandemic Preparedness." Clinical and Vaccine Immunology : CVI, vol. 14, no. 11, 2007, pp. 1425-32.
Pappas C, Matsuoka Y, Swayne DE, et al. Development and evaluation of an Influenza virus subtype H7N2 vaccine candidate for pandemic preparedness. Clin Vaccine Immunol. 2007;14(11):1425-32.
Pappas, C., Matsuoka, Y., Swayne, D. E., & Donis, R. O. (2007). Development and evaluation of an Influenza virus subtype H7N2 vaccine candidate for pandemic preparedness. Clinical and Vaccine Immunology : CVI, 14(11), 1425-32.
Pappas C, et al. Development and Evaluation of an Influenza Virus Subtype H7N2 Vaccine Candidate for Pandemic Preparedness. Clin Vaccine Immunol. 2007;14(11):1425-32. PubMed PMID: 17913860.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Development and evaluation of an Influenza virus subtype H7N2 vaccine candidate for pandemic preparedness. AU - Pappas,Claudia, AU - Matsuoka,Yumiko, AU - Swayne,David E, AU - Donis,Ruben O, Y1 - 2007/10/03/ PY - 2007/10/5/pubmed PY - 2008/1/15/medline PY - 2007/10/5/entrez SP - 1425 EP - 32 JF - Clinical and vaccine immunology : CVI JO - Clin Vaccine Immunol VL - 14 IS - 11 N2 - Influenza virus of the H7N2 subtype has been introduced into noncommercial poultry in the United States, and this probably resulted in incidents of transmission of H7N2 virus to humans, documented in 2002 and 2003. This virus could be considered a potential threat to public health if it acquired person-to-person transmissibility. A favored approach for global pandemic preparedness includes development of prepandemic vaccines for any potential pandemic virus. To this end, we created a high-growth reassortant virus (H7N2-PR8) containing the genes for the hemagglutinin and the neuraminidase from a low-pathogenicity (H7N2) virus strain and the remaining six genes from a human vaccine strain (H1N1). The reassortant strain was evaluated to assess its antigenicity, safety, and protective efficacy using a mouse model. Antigenicity studies using ferret antibodies raised against H7N2-PR8 indicated that this virus confers broad cross-reactivity with divergent H7 viruses of different years and lineages. Mice and chickens inoculated with high doses of H7N2-PR8 supported virus replication but survived, indicating that this virus is comparable to other avian viruses of low pathogenicity. To assess the protective efficacy of H7N2-PR8, mice were immunized with two doses of formalin-inactivated H7N2-PR8, alone or with alum. Vaccinated mice subsequently challenged with highly pathogenic viruses from homologous and heterologous lineages A/Canada/444/04 (H7N3) and A/Netherlands/219/03 (H7N7) showed pronounced reduction of wild-type virus replication. These studies indicate that H7N2-PR8 is immunogenic, safe, and protective in animal models; these are the essential attributes to qualify for phase I human clinical trials as a prepandemic vaccine. SN - 1556-6811 UR - https://www.unboundmedicine.com/medline/citation/17913860/Development_and_evaluation_of_an_Influenza_virus_subtype_H7N2_vaccine_candidate_for_pandemic_preparedness_ L2 - http://cvi.asm.org/cgi/pmidlookup?view=long&pmid=17913860 DB - PRIME DP - Unbound Medicine ER -