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Environmental factors of celiac disease: cytotoxicity of hulled wheat species Triticum monococcum, T. turgidum ssp. dicoccum and T. aestivum ssp. spelta.
J Gastroenterol Hepatol. 2007 Nov; 22(11):1816-22.JG

Abstract

BACKGROUND AND AIM

In the present paper, the toxicity of prolamines derived from three cereals with a different genome was investigated in human colon cancer Caco-2/TC7 and human myelogenous leukemia K562(S) cells. The purpose of this study was to investigate if species from ancient wheat could be considered as healthy food crops devoid or poor in cytotoxic prolamines for celiac disease.

METHODS

Cytotoxicity was measured in terms of inhibition of cell growth, activation of apoptosis, release of nitric oxide (NO), detection of tissue transglutaminase (TG II) and alteration of transepithelial electrical resistance (TEER) on Caco-2/Tc7 and K562 (S) cell agglutination. Peptic-tryptic (PT) digest from bread wheat (T. aestivum S. Pastore) was used as a positive control.

RESULTS

PT digests of prolamins from spelt wheat (T. aestivum ssp. spelta) were found to exert toxic effects on Caco-2/TC7 cells and to agglutinate K562(S) cells. Increased amounts of NO and TG II expression were observed in Caco-2/TC7 cells exposed to 1 mg/mL of spelt prolamins, suggesting that spelt wheat can induce cellular mechanisms implicated in the pathogenesis of celiac disease. By contrast, the PT digests from monoccum wheat (Triticum monococcum) and farro wheat (T. turgidum ssp. dicoccum) did not exhibit any negative effects on Caco-2/TC7 and K562(S) cells.

CONCLUSIONS

The results have shown a constant and significant toxic effect of spelt wheat which is not shared by the two other ancient cereals. Future studies on celiac intestinal organ cultures are needed to increase the prospects of breeding programs aimed at developing wheat cultivars potentially tolerated by most celiac patients.

Authors+Show Affiliations

Division of Food Science, Human Nutrition and Health, National Institute of Health, Rome, Italy.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

17914956

Citation

Vincentini, Olimpia, et al. "Environmental Factors of Celiac Disease: Cytotoxicity of Hulled Wheat Species Triticum Monococcum, T. Turgidum Ssp. Dicoccum and T. Aestivum Ssp. Spelta." Journal of Gastroenterology and Hepatology, vol. 22, no. 11, 2007, pp. 1816-22.
Vincentini O, Maialetti F, Gazza L, et al. Environmental factors of celiac disease: cytotoxicity of hulled wheat species Triticum monococcum, T. turgidum ssp. dicoccum and T. aestivum ssp. spelta. J Gastroenterol Hepatol. 2007;22(11):1816-22.
Vincentini, O., Maialetti, F., Gazza, L., Silano, M., Dessi, M., De Vincenzi, M., & Pogna, N. E. (2007). Environmental factors of celiac disease: cytotoxicity of hulled wheat species Triticum monococcum, T. turgidum ssp. dicoccum and T. aestivum ssp. spelta. Journal of Gastroenterology and Hepatology, 22(11), 1816-22.
Vincentini O, et al. Environmental Factors of Celiac Disease: Cytotoxicity of Hulled Wheat Species Triticum Monococcum, T. Turgidum Ssp. Dicoccum and T. Aestivum Ssp. Spelta. J Gastroenterol Hepatol. 2007;22(11):1816-22. PubMed PMID: 17914956.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Environmental factors of celiac disease: cytotoxicity of hulled wheat species Triticum monococcum, T. turgidum ssp. dicoccum and T. aestivum ssp. spelta. AU - Vincentini,Olimpia, AU - Maialetti,Francesca, AU - Gazza,Laura, AU - Silano,Marco, AU - Dessi,Mariarita, AU - De Vincenzi,Massimo, AU - Pogna,Norberto Edgardo, PY - 2007/10/5/pubmed PY - 2008/1/19/medline PY - 2007/10/5/entrez SP - 1816 EP - 22 JF - Journal of gastroenterology and hepatology JO - J Gastroenterol Hepatol VL - 22 IS - 11 N2 - BACKGROUND AND AIM: In the present paper, the toxicity of prolamines derived from three cereals with a different genome was investigated in human colon cancer Caco-2/TC7 and human myelogenous leukemia K562(S) cells. The purpose of this study was to investigate if species from ancient wheat could be considered as healthy food crops devoid or poor in cytotoxic prolamines for celiac disease. METHODS: Cytotoxicity was measured in terms of inhibition of cell growth, activation of apoptosis, release of nitric oxide (NO), detection of tissue transglutaminase (TG II) and alteration of transepithelial electrical resistance (TEER) on Caco-2/Tc7 and K562 (S) cell agglutination. Peptic-tryptic (PT) digest from bread wheat (T. aestivum S. Pastore) was used as a positive control. RESULTS: PT digests of prolamins from spelt wheat (T. aestivum ssp. spelta) were found to exert toxic effects on Caco-2/TC7 cells and to agglutinate K562(S) cells. Increased amounts of NO and TG II expression were observed in Caco-2/TC7 cells exposed to 1 mg/mL of spelt prolamins, suggesting that spelt wheat can induce cellular mechanisms implicated in the pathogenesis of celiac disease. By contrast, the PT digests from monoccum wheat (Triticum monococcum) and farro wheat (T. turgidum ssp. dicoccum) did not exhibit any negative effects on Caco-2/TC7 and K562(S) cells. CONCLUSIONS: The results have shown a constant and significant toxic effect of spelt wheat which is not shared by the two other ancient cereals. Future studies on celiac intestinal organ cultures are needed to increase the prospects of breeding programs aimed at developing wheat cultivars potentially tolerated by most celiac patients. SN - 0815-9319 UR - https://www.unboundmedicine.com/medline/citation/17914956/Environmental_factors_of_celiac_disease:_cytotoxicity_of_hulled_wheat_species_Triticum_monococcum_T__turgidum_ssp__dicoccum_and_T__aestivum_ssp__spelta_ L2 - https://doi.org/10.1111/j.1440-1746.2006.04680.x DB - PRIME DP - Unbound Medicine ER -