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Development of an integrated direct-contacting optical-fiber microchip with light-emitting diode-induced fluorescence detection.
J Chromatogr A. 2007 Nov 02; 1170(1-2):101-6.JC

Abstract

In this paper, one poly(dimethylsiloxane) (PDMS) sandwich microchip integrated with one direct-contacting optical fiber was fabricated by using a thin-casting method. This novel integrated PDMS sandwich microchip included top glass plate, PDMS membrane replica with microfluidic networks and optical fiber, flat PDMS membrane and bottom glass plate. As the tip of excitation optical fiber completely contacted with the separation microchannel in this integrated microchip, it not only increased the excitation light intensity to achieve the high sensitivity, but also reduced the diameter of excitation beam to obtain high resolution. In addition, we found that this rigid PDMS sandwich microchip structure effectively prevented PDMS microchannel distortion from rigid optical fiber, and provided a substantial convenience for microchips manipulating. A blue light-emitting diode (LED) was applied as excitation source by using optical fiber to couple excitation light into its direct-contacting microchannel for fluorescence detection. The performances of this integrated PDMS sandwich microchip was demonstrated by separating the mixture of sodium fluorescein (SF) and fluorescein isothiocyanate isomer I (FITC), and showed a higher sensitive and resolution than those obtained from the conventional integrated optical-fiber PDMS microchip with a 100-microm distance between fiber tip and separation microchannel. Additionally, the reproducibility of this integrated microchip with LED-induced fluorescence detection was also examined by separation of a mixture of FITC-labeled amino acids.

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Authors+Show Affiliations

Liu C
State Key Laboratory of Transducer Technology, Institute of Electronics, Chinese Academy of Sciences, Beijing 100080, China. liucc263@yahoo.com.cn
Cui D
No affiliation info available
Chen X
No affiliation info available

MeSH

Amino AcidsCoated Materials, BiocompatibleDimethylpolysiloxanesElectrophoresis, MicrochipFiber Optic TechnologyFluoresceinIsothiocyanatesLightMicrofluidicsOptical FibersReproducibility of ResultsSensitivity and SpecificitySpectrometry, Fluorescence

Pub Type(s)

Evaluation Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

17915241

Citation

Liu, Changchun, et al. "Development of an Integrated Direct-contacting Optical-fiber Microchip With Light-emitting Diode-induced Fluorescence Detection." Journal of Chromatography. A, vol. 1170, no. 1-2, 2007, pp. 101-6.
Liu C, Cui D, Chen X. Development of an integrated direct-contacting optical-fiber microchip with light-emitting diode-induced fluorescence detection. J Chromatogr A. 2007;1170(1-2):101-6.
Liu, C., Cui, D., & Chen, X. (2007). Development of an integrated direct-contacting optical-fiber microchip with light-emitting diode-induced fluorescence detection. Journal of Chromatography. A, 1170(1-2), 101-6.
Liu C, Cui D, Chen X. Development of an Integrated Direct-contacting Optical-fiber Microchip With Light-emitting Diode-induced Fluorescence Detection. J Chromatogr A. 2007 Nov 2;1170(1-2):101-6. PubMed PMID: 17915241.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Development of an integrated direct-contacting optical-fiber microchip with light-emitting diode-induced fluorescence detection. AU - Liu,Changchun, AU - Cui,Dafu, AU - Chen,Xing, Y1 - 2007/09/12/ PY - 2007/05/09/received PY - 2007/08/26/revised PY - 2007/08/31/accepted PY - 2007/10/5/pubmed PY - 2008/2/27/medline PY - 2007/10/5/entrez SP - 101 EP - 6 JF - Journal of chromatography. A JO - J Chromatogr A VL - 1170 IS - 1-2 N2 - In this paper, one poly(dimethylsiloxane) (PDMS) sandwich microchip integrated with one direct-contacting optical fiber was fabricated by using a thin-casting method. This novel integrated PDMS sandwich microchip included top glass plate, PDMS membrane replica with microfluidic networks and optical fiber, flat PDMS membrane and bottom glass plate. As the tip of excitation optical fiber completely contacted with the separation microchannel in this integrated microchip, it not only increased the excitation light intensity to achieve the high sensitivity, but also reduced the diameter of excitation beam to obtain high resolution. In addition, we found that this rigid PDMS sandwich microchip structure effectively prevented PDMS microchannel distortion from rigid optical fiber, and provided a substantial convenience for microchips manipulating. A blue light-emitting diode (LED) was applied as excitation source by using optical fiber to couple excitation light into its direct-contacting microchannel for fluorescence detection. The performances of this integrated PDMS sandwich microchip was demonstrated by separating the mixture of sodium fluorescein (SF) and fluorescein isothiocyanate isomer I (FITC), and showed a higher sensitive and resolution than those obtained from the conventional integrated optical-fiber PDMS microchip with a 100-microm distance between fiber tip and separation microchannel. Additionally, the reproducibility of this integrated microchip with LED-induced fluorescence detection was also examined by separation of a mixture of FITC-labeled amino acids. SN - 0021-9673 UR - https://www.unboundmedicine.com/medline/citation/17915241/Development_of_an_integrated_direct_contacting_optical_fiber_microchip_with_light_emitting_diode_induced_fluorescence_detection_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0021-9673(07)01503-8 DB - PRIME DP - Unbound Medicine ER -