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Variants of eae and stx genes of atypical enteropathogenic Escherichia coli and non-O157 Shiga toxin-producing Escherichia coli from calves.
Lett Appl Microbiol. 2007 Dec; 45(6):610-5.LA

Abstract

AIMS

To determine the subtypes of stx and eae genes of Shiga toxin-producing Escherichia coli (STEC) and enteropathogenic Escherichia coli (EPEC) from calves and to ascertain the typical and atypical nature of EPEC.

METHODS AND RESULTS

One hundred and eighty-seven faecal samples from 134 diarrhoeic and 53 healthy calves were investigated for the presence of stx, eae and ehxA virulence genes by polymerase chain reaction and enzyme-linked immunosorbent assay. Subtype analysis of stx(1) exhibited stx(1c) in 13 (31.70%) isolates, while that of stx(2) revealed stx(2c) in eight (24.24%) and stx(2d) in two (6.06%) isolates. Subtyping of eae gene showed the presence of eae-beta, eae-eta and eae-zeta in two, three and four isolates respectively. None of the E. coli isolates possessed stx(2e), stx(2f), eae-alpha, eae-delta, eae-epsilon and eae-xi. All EPEC isolates were atypical.

CONCLUSIONS

stx(1), stx(1c), stx(2), stx(2c), stx(2d), eae-beta, eae-eta and eae-zeta subtypes are prevalent in STEC and EPEC isolates in India.

SIGNIFICANCE AND IMPACT OF THE STUDY

This is the first subtype analysis of stx(2) and eae genes of animal E. coli isolates in India and emphasizes the need to investigate their transmission to humans.

Authors+Show Affiliations

Bacteriology Laboratory, Division of Veterinary Microbiology and Immunology, SK University of Agricultural Sciences and Technology of Kashmir, Shuhama (Alusteng), Srinagar, India. shakilwani@gmail.comNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

17916128

Citation

Wani, S A., et al. "Variants of Eae and Stx Genes of Atypical Enteropathogenic Escherichia Coli and non-O157 Shiga Toxin-producing Escherichia Coli From Calves." Letters in Applied Microbiology, vol. 45, no. 6, 2007, pp. 610-5.
Wani SA, Hussain I, Nabi A, et al. Variants of eae and stx genes of atypical enteropathogenic Escherichia coli and non-O157 Shiga toxin-producing Escherichia coli from calves. Lett Appl Microbiol. 2007;45(6):610-5.
Wani, S. A., Hussain, I., Nabi, A., Fayaz, I., & Nishikawa, Y. (2007). Variants of eae and stx genes of atypical enteropathogenic Escherichia coli and non-O157 Shiga toxin-producing Escherichia coli from calves. Letters in Applied Microbiology, 45(6), 610-5.
Wani SA, et al. Variants of Eae and Stx Genes of Atypical Enteropathogenic Escherichia Coli and non-O157 Shiga Toxin-producing Escherichia Coli From Calves. Lett Appl Microbiol. 2007;45(6):610-5. PubMed PMID: 17916128.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Variants of eae and stx genes of atypical enteropathogenic Escherichia coli and non-O157 Shiga toxin-producing Escherichia coli from calves. AU - Wani,S A, AU - Hussain,I, AU - Nabi,A, AU - Fayaz,I, AU - Nishikawa,Y, Y1 - 2007/10/04/ PY - 2007/10/6/pubmed PY - 2008/1/23/medline PY - 2007/10/6/entrez SP - 610 EP - 5 JF - Letters in applied microbiology JO - Lett Appl Microbiol VL - 45 IS - 6 N2 - AIMS: To determine the subtypes of stx and eae genes of Shiga toxin-producing Escherichia coli (STEC) and enteropathogenic Escherichia coli (EPEC) from calves and to ascertain the typical and atypical nature of EPEC. METHODS AND RESULTS: One hundred and eighty-seven faecal samples from 134 diarrhoeic and 53 healthy calves were investigated for the presence of stx, eae and ehxA virulence genes by polymerase chain reaction and enzyme-linked immunosorbent assay. Subtype analysis of stx(1) exhibited stx(1c) in 13 (31.70%) isolates, while that of stx(2) revealed stx(2c) in eight (24.24%) and stx(2d) in two (6.06%) isolates. Subtyping of eae gene showed the presence of eae-beta, eae-eta and eae-zeta in two, three and four isolates respectively. None of the E. coli isolates possessed stx(2e), stx(2f), eae-alpha, eae-delta, eae-epsilon and eae-xi. All EPEC isolates were atypical. CONCLUSIONS: stx(1), stx(1c), stx(2), stx(2c), stx(2d), eae-beta, eae-eta and eae-zeta subtypes are prevalent in STEC and EPEC isolates in India. SIGNIFICANCE AND IMPACT OF THE STUDY: This is the first subtype analysis of stx(2) and eae genes of animal E. coli isolates in India and emphasizes the need to investigate their transmission to humans. SN - 0266-8254 UR - https://www.unboundmedicine.com/medline/citation/17916128/Variants_of_eae_and_stx_genes_of_atypical_enteropathogenic_Escherichia_coli_and_non_O157_Shiga_toxin_producing_Escherichia_coli_from_calves_ L2 - https://doi.org/10.1111/j.1472-765X.2007.02235.x DB - PRIME DP - Unbound Medicine ER -