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Identification of enzyme activity that conjugates indole-3-acetic acid to aspartate in immature seeds of pea (Pisum sativum).
J Plant Physiol. 2008; 165(5):564-9.JP

Abstract

This study describes the first identification of plant enzyme activity catalyzing the conjugation of indole-3-acetic acid to amino acids. Enzymatic synthesis of indole-3-acetylaspartate (IAA-Asp) by a crude enzyme preparation from immature seeds of pea (Pisum sativum) was observed. The reaction yielded a product with the same Rf as IAA-Asp standard after thin layer chromatography. The identity of IAA-Asp was verified by HPLC analysis. IAA-Asp formation was dependent on ATP and Mg2+, and was linear during a 60 min period. The enzyme preparation obtained after poly(ethylene glycol) 6000 fractionation showed optimum activity at pH 8.0, and the temperature optimum for IAA-Asp synthesis was 30 degrees C.

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Authors+Show Affiliations

Ostrowski M
Department of Biochemistry, Nicolaus Copernicus University, Institute of General and Molecular Biology, ul. Gagarina 9, 87-100 Toruń, Poland. maciejo@stud.umk.pl
Jakubowska A
No affiliation info available

MeSH

Aspartic AcidChromatography, High Pressure LiquidChromatography, Thin LayerHydrogen-Ion ConcentrationIndoleacetic AcidsLigasesPeasPlant ProteinsSeeds

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

17920159

Citation

Ostrowski, Maciej, and Anna Jakubowska. "Identification of Enzyme Activity That Conjugates Indole-3-acetic Acid to Aspartate in Immature Seeds of Pea (Pisum Sativum)." Journal of Plant Physiology, vol. 165, no. 5, 2008, pp. 564-9.
Ostrowski M, Jakubowska A. Identification of enzyme activity that conjugates indole-3-acetic acid to aspartate in immature seeds of pea (Pisum sativum). J Plant Physiol. 2008;165(5):564-9.
Ostrowski, M., & Jakubowska, A. (2008). Identification of enzyme activity that conjugates indole-3-acetic acid to aspartate in immature seeds of pea (Pisum sativum). Journal of Plant Physiology, 165(5), 564-9.
Ostrowski M, Jakubowska A. Identification of Enzyme Activity That Conjugates Indole-3-acetic Acid to Aspartate in Immature Seeds of Pea (Pisum Sativum). J Plant Physiol. 2008;165(5):564-9. PubMed PMID: 17920159.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Identification of enzyme activity that conjugates indole-3-acetic acid to aspartate in immature seeds of pea (Pisum sativum). AU - Ostrowski,Maciej, AU - Jakubowska,Anna, Y1 - 2007/10/24/ PY - 2007/04/30/received PY - 2007/07/03/revised PY - 2007/07/05/accepted PY - 2007/10/9/pubmed PY - 2008/7/22/medline PY - 2007/10/9/entrez SP - 564 EP - 9 JF - Journal of plant physiology JO - J Plant Physiol VL - 165 IS - 5 N2 - This study describes the first identification of plant enzyme activity catalyzing the conjugation of indole-3-acetic acid to amino acids. Enzymatic synthesis of indole-3-acetylaspartate (IAA-Asp) by a crude enzyme preparation from immature seeds of pea (Pisum sativum) was observed. The reaction yielded a product with the same Rf as IAA-Asp standard after thin layer chromatography. The identity of IAA-Asp was verified by HPLC analysis. IAA-Asp formation was dependent on ATP and Mg2+, and was linear during a 60 min period. The enzyme preparation obtained after poly(ethylene glycol) 6000 fractionation showed optimum activity at pH 8.0, and the temperature optimum for IAA-Asp synthesis was 30 degrees C. SN - 0176-1617 UR - https://www.unboundmedicine.com/medline/citation/17920159/Identification_of_enzyme_activity_that_conjugates_indole_3_acetic_acid_to_aspartate_in_immature_seeds_of_pea__Pisum_sativum__ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0176-1617(07)00184-8 DB - PRIME DP - Unbound Medicine ER -