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Inhibitory effects of Stewartia koreana on osteoclast differentiation and bone resorption.
Int Immunopharmacol. 2007 Dec 05; 7(12):1507-16.II

Abstract

Osteoclasts are responsible for bone lysis in several bone diseases such as osteoporosis and rheumatoid arthritis. Natural products from plants have been invaluable source in discovery of compounds for new therapies. In this study, we screened plant products for potential application to therapy for bone loss using a primary osteoclastogenesis culture system and found that extract of Stewartia koreana (SKE) had a strong inhibitory effect on osteoclast formation. To gain molecular insights, we examined the effect of SKE on signaling pathways and transcription factors stimulated by the osteoclast differentiation factor RANKL. SKE suppressed the induction of c-Fos and NFATc1 by RANKL. However, SKE did not inhibit NF-kappaB activation by RANKL. Among the MAPKs stimulated by RANKL, SKE significantly reduced the activation of ERK and p38. Therefore, the anti-osteoclastogenic effect of SKE is likely to be elicited by interference with RANKL signaling to ERK and p38, which mediate the induction of c-Fos and subsequently that of NFATc1. Consistent with the in vitro effect on osteoclast differentiation, SKE showed a great inhibitory effect on in vivo bone loss in LPS-challenged mice. Taken together, we demonstrated that SKE has inhibitory effects on osteoclast differentiation in vitro and confirmed its in vivo efficacy in prevention of inflammatory bone loss.

Authors+Show Affiliations

Department of Cell and Developmental Biology, BK21 Program, Dental Research Institute, Seoul National University School of Dentistry, 28 Yeongon-Dong, Chongno-Gu, Seoul, 110-749, South Korea.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

17920527

Citation

Park, Cheol Kyu, et al. "Inhibitory Effects of Stewartia Koreana On Osteoclast Differentiation and Bone Resorption." International Immunopharmacology, vol. 7, no. 12, 2007, pp. 1507-16.
Park CK, Kim HJ, Kwak HB, et al. Inhibitory effects of Stewartia koreana on osteoclast differentiation and bone resorption. Int Immunopharmacol. 2007;7(12):1507-16.
Park, C. K., Kim, H. J., Kwak, H. B., Lee, T. H., Bang, M. H., Kim, C. M., Lee, Y., Chung, D. K., Baek, N. I., Kim, J., Lee, Z. H., & Kim, H. H. (2007). Inhibitory effects of Stewartia koreana on osteoclast differentiation and bone resorption. International Immunopharmacology, 7(12), 1507-16.
Park CK, et al. Inhibitory Effects of Stewartia Koreana On Osteoclast Differentiation and Bone Resorption. Int Immunopharmacol. 2007 Dec 5;7(12):1507-16. PubMed PMID: 17920527.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Inhibitory effects of Stewartia koreana on osteoclast differentiation and bone resorption. AU - Park,Cheol Kyu, AU - Kim,Hyung Joon, AU - Kwak,Han Bok, AU - Lee,Tae Hoon, AU - Bang,Myun-Ho, AU - Kim,Chul Min, AU - Lee,Youngkyun, AU - Chung,Dae Kyun, AU - Baek,Nam-In, AU - Kim,Jiyoung, AU - Lee,Zang Hee, AU - Kim,Hong-Hee, Y1 - 2007/08/08/ PY - 2007/04/09/received PY - 2007/07/10/revised PY - 2007/07/12/accepted PY - 2007/10/9/pubmed PY - 2008/1/11/medline PY - 2007/10/9/entrez SP - 1507 EP - 16 JF - International immunopharmacology JO - Int Immunopharmacol VL - 7 IS - 12 N2 - Osteoclasts are responsible for bone lysis in several bone diseases such as osteoporosis and rheumatoid arthritis. Natural products from plants have been invaluable source in discovery of compounds for new therapies. In this study, we screened plant products for potential application to therapy for bone loss using a primary osteoclastogenesis culture system and found that extract of Stewartia koreana (SKE) had a strong inhibitory effect on osteoclast formation. To gain molecular insights, we examined the effect of SKE on signaling pathways and transcription factors stimulated by the osteoclast differentiation factor RANKL. SKE suppressed the induction of c-Fos and NFATc1 by RANKL. However, SKE did not inhibit NF-kappaB activation by RANKL. Among the MAPKs stimulated by RANKL, SKE significantly reduced the activation of ERK and p38. Therefore, the anti-osteoclastogenic effect of SKE is likely to be elicited by interference with RANKL signaling to ERK and p38, which mediate the induction of c-Fos and subsequently that of NFATc1. Consistent with the in vitro effect on osteoclast differentiation, SKE showed a great inhibitory effect on in vivo bone loss in LPS-challenged mice. Taken together, we demonstrated that SKE has inhibitory effects on osteoclast differentiation in vitro and confirmed its in vivo efficacy in prevention of inflammatory bone loss. SN - 1567-5769 UR - https://www.unboundmedicine.com/medline/citation/17920527/Inhibitory_effects_of_Stewartia_koreana_on_osteoclast_differentiation_and_bone_resorption_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S1567-5769(07)00207-X DB - PRIME DP - Unbound Medicine ER -