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Studies on the antioxidant activity of the essential oil and methanol extract of Marrubium globosum subsp. globosum (lamiaceae) by three different chemical assays.
Bioresour Technol. 2008 Jul; 99(10):4239-46.BT

Abstract

This study is designed to examine the chemical composition and in vitro antioxidant activity of the essential oil and sub-fractions of the methanol extract of Marrubium globosum subsp. globosum. The GC and GC-MS analysis of the essential oil were resulted in the determination of 84 components representing 88.2% of the oil. The major constituents of the oil were spathulenol (15.8%), beta-caryophyllene (9.0%), caryophyllene oxide (7.9%), germacrene D (6.5%), and bicyclogermacrene (3.1%). Antioxidant activities of the samples were determined by three different test systems namely DPPH, beta-carotene/linoleic acid and reducing power assay. In DPPH system, the weakest radical scavenging activity was exhibited by the essential oil (1203.38+/-7.18 microg ml(-1)). Antioxidant activity of the polar sub-fraction of methanol extract was superior to the all samples tested with an EC(50) value of 157.26+/-1.12 microg ml(-1). In the second case, the inhibition capacity (%) of the polar sub-fraction of methanol extract (97.39%+/-0.84) was found the strongest one, which is almost equal to the inhibition capacity of positive control BHT (97.44%+/-0.74). In the case of reducing power assay, a similar activity pattern was observed as given in the first two systems. Polar sub-fraction was the strongest radical reducer when compared with the non-polar one, with an EC(50) value of 625.63+/-1.02 microg ml(-1). The amount of the total phenolics was highest in polar sub-fraction (25.60+/-0.74 microg/mg). A positive correlation was observed between the antioxidant activity potential and total phenolic level of the extracts. On the other hand, total flavonoid content was found equal for the both sub-fractions.

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Authors+Show Affiliations

Sarikurkcu C
Faculty of Science and Literature, Department of Chemistry, Mugla University, Mugla 48000, Turkey.
Tepe B
No affiliation info available
Daferera D
No affiliation info available
Polissiou M
No affiliation info available
Harmandar M
No affiliation info available

MeSH

AntioxidantsBiotechnologyBiphenyl CompoundsChromatography, GasFlavonoidsGas Chromatography-Mass SpectrometryHydrazinesLamiaceaeLinoleic AcidMarrubiumMethanolOils, VolatilePhenolPicratesPlant Extractsbeta Carotene

Pub Type(s)

Journal Article

Language

eng

PubMed ID

17920880

Citation

Sarikurkcu, Cengiz, et al. "Studies On the Antioxidant Activity of the Essential Oil and Methanol Extract of Marrubium Globosum Subsp. Globosum (lamiaceae) By Three Different Chemical Assays." Bioresource Technology, vol. 99, no. 10, 2008, pp. 4239-46.
Sarikurkcu C, Tepe B, Daferera D, et al. Studies on the antioxidant activity of the essential oil and methanol extract of Marrubium globosum subsp. globosum (lamiaceae) by three different chemical assays. Bioresour Technol. 2008;99(10):4239-46.
Sarikurkcu, C., Tepe, B., Daferera, D., Polissiou, M., & Harmandar, M. (2008). Studies on the antioxidant activity of the essential oil and methanol extract of Marrubium globosum subsp. globosum (lamiaceae) by three different chemical assays. Bioresource Technology, 99(10), 4239-46.
Sarikurkcu C, et al. Studies On the Antioxidant Activity of the Essential Oil and Methanol Extract of Marrubium Globosum Subsp. Globosum (lamiaceae) By Three Different Chemical Assays. Bioresour Technol. 2008;99(10):4239-46. PubMed PMID: 17920880.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Studies on the antioxidant activity of the essential oil and methanol extract of Marrubium globosum subsp. globosum (lamiaceae) by three different chemical assays. AU - Sarikurkcu,Cengiz, AU - Tepe,Bektas, AU - Daferera,Dimitra, AU - Polissiou,Moschos, AU - Harmandar,Mansur, Y1 - 2007/10/24/ PY - 2007/03/29/received PY - 2007/05/14/revised PY - 2007/08/25/accepted PY - 2007/10/9/pubmed PY - 2008/6/17/medline PY - 2007/10/9/entrez SP - 4239 EP - 46 JF - Bioresource technology JO - Bioresour Technol VL - 99 IS - 10 N2 - This study is designed to examine the chemical composition and in vitro antioxidant activity of the essential oil and sub-fractions of the methanol extract of Marrubium globosum subsp. globosum. The GC and GC-MS analysis of the essential oil were resulted in the determination of 84 components representing 88.2% of the oil. The major constituents of the oil were spathulenol (15.8%), beta-caryophyllene (9.0%), caryophyllene oxide (7.9%), germacrene D (6.5%), and bicyclogermacrene (3.1%). Antioxidant activities of the samples were determined by three different test systems namely DPPH, beta-carotene/linoleic acid and reducing power assay. In DPPH system, the weakest radical scavenging activity was exhibited by the essential oil (1203.38+/-7.18 microg ml(-1)). Antioxidant activity of the polar sub-fraction of methanol extract was superior to the all samples tested with an EC(50) value of 157.26+/-1.12 microg ml(-1). In the second case, the inhibition capacity (%) of the polar sub-fraction of methanol extract (97.39%+/-0.84) was found the strongest one, which is almost equal to the inhibition capacity of positive control BHT (97.44%+/-0.74). In the case of reducing power assay, a similar activity pattern was observed as given in the first two systems. Polar sub-fraction was the strongest radical reducer when compared with the non-polar one, with an EC(50) value of 625.63+/-1.02 microg ml(-1). The amount of the total phenolics was highest in polar sub-fraction (25.60+/-0.74 microg/mg). A positive correlation was observed between the antioxidant activity potential and total phenolic level of the extracts. On the other hand, total flavonoid content was found equal for the both sub-fractions. SN - 0960-8524 UR - https://www.unboundmedicine.com/medline/citation/17920880/Studies_on_the_antioxidant_activity_of_the_essential_oil_and_methanol_extract_of_Marrubium_globosum_subsp__globosum__lamiaceae__by_three_different_chemical_assays_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0960-8524(07)00720-1 DB - PRIME DP - Unbound Medicine ER -