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The effects of platelet-activating factor on the secretion of interleukin-8 and growth-regulated oncogene alpha in human immortalized granulosa cell line (GC1a).
Am J Reprod Immunol. 2007 Nov; 58(5):434-9.AJ

Abstract

PROBLEM

To investigate the role of platelet-activating factor (PAF) in human ovulation, we studied the regulation of interleukin (IL)-8 and growth-regulated oncogene (GRO) alpha in cultured human immortalized granulosa cell line (GC1a).

METHOD OF STUDY

GC1a was cultured in serum-free medium, and incubated with carbamyl-PAF (C-PAF) and/or PAF receptor antagonist (WEB 2086). The supernatants were collected, and IL-8 and GRO alpha were measured by enzyme-linked immunosorbent assay.

RESULTS

After treatment with C-PAF, the levels of IL-8 and GROalpha increased in a time-dependent manner. The levels of IL-8 and GROalpha were significantly increased after treatment with C-PAF in a dose-dependent manner. However, the levels of IL-8 and GROalpha were significantly decreased by treatment with C-PAF and with increasing concentrations of WEB 2086.

CONCLUSION

Our data indicated that IL-8 and GROalpha were regulated by C-PAF. The results suggested that PAF may play an important role in human pre-ovulatory processes involving IL-8 and GROalpha production.

Authors+Show Affiliations

Department of Obstetrics and Gynecology, Faculty of Medicine, Oita University, Oita, Japan. kawayas@med.oita-u.ac.jpNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

17922696

Citation

Kawano, Yasushi, et al. "The Effects of Platelet-activating Factor On the Secretion of Interleukin-8 and Growth-regulated Oncogene Alpha in Human Immortalized Granulosa Cell Line (GC1a)." American Journal of Reproductive Immunology (New York, N.Y. : 1989), vol. 58, no. 5, 2007, pp. 434-9.
Kawano Y, Furukawa Y, Fukuda J, et al. The effects of platelet-activating factor on the secretion of interleukin-8 and growth-regulated oncogene alpha in human immortalized granulosa cell line (GC1a). Am J Reprod Immunol. 2007;58(5):434-9.
Kawano, Y., Furukawa, Y., Fukuda, J., Matsumoto, H., Yuge, A., & Narahara, H. (2007). The effects of platelet-activating factor on the secretion of interleukin-8 and growth-regulated oncogene alpha in human immortalized granulosa cell line (GC1a). American Journal of Reproductive Immunology (New York, N.Y. : 1989), 58(5), 434-9.
Kawano Y, et al. The Effects of Platelet-activating Factor On the Secretion of Interleukin-8 and Growth-regulated Oncogene Alpha in Human Immortalized Granulosa Cell Line (GC1a). Am J Reprod Immunol. 2007;58(5):434-9. PubMed PMID: 17922696.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - The effects of platelet-activating factor on the secretion of interleukin-8 and growth-regulated oncogene alpha in human immortalized granulosa cell line (GC1a). AU - Kawano,Yasushi, AU - Furukawa,Yuichi, AU - Fukuda,Junichiro, AU - Matsumoto,Harunobu, AU - Yuge,Akitoshi, AU - Narahara,Hisashi, PY - 2007/10/10/pubmed PY - 2007/12/21/medline PY - 2007/10/10/entrez SP - 434 EP - 9 JF - American journal of reproductive immunology (New York, N.Y. : 1989) JO - Am J Reprod Immunol VL - 58 IS - 5 N2 - PROBLEM: To investigate the role of platelet-activating factor (PAF) in human ovulation, we studied the regulation of interleukin (IL)-8 and growth-regulated oncogene (GRO) alpha in cultured human immortalized granulosa cell line (GC1a). METHOD OF STUDY: GC1a was cultured in serum-free medium, and incubated with carbamyl-PAF (C-PAF) and/or PAF receptor antagonist (WEB 2086). The supernatants were collected, and IL-8 and GRO alpha were measured by enzyme-linked immunosorbent assay. RESULTS: After treatment with C-PAF, the levels of IL-8 and GROalpha increased in a time-dependent manner. The levels of IL-8 and GROalpha were significantly increased after treatment with C-PAF in a dose-dependent manner. However, the levels of IL-8 and GROalpha were significantly decreased by treatment with C-PAF and with increasing concentrations of WEB 2086. CONCLUSION: Our data indicated that IL-8 and GROalpha were regulated by C-PAF. The results suggested that PAF may play an important role in human pre-ovulatory processes involving IL-8 and GROalpha production. SN - 1046-7408 UR - https://www.unboundmedicine.com/medline/citation/17922696/The_effects_of_platelet_activating_factor_on_the_secretion_of_interleukin_8_and_growth_regulated_oncogene_alpha_in_human_immortalized_granulosa_cell_line__GC1a__ L2 - https://doi.org/10.1111/j.1600-0897.2007.00527.x DB - PRIME DP - Unbound Medicine ER -