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Dehydroepiandrosterone treatment attenuates reperfusion injury after testicular torsion and detorsion in rats.
J Pediatr Surg. 2007 Oct; 42(10):1740-4.JP

Abstract

PURPOSE

We aimed to evaluate the effects of dehydroepiandrosterone (DHEA) on antioxidant enzyme activities, lipid peroxidation, and histopathologic changes in both testes after unilateral testicular torsion and detorsion.

METHODS

Twenty-four adult male Sprague-Dawley rats were randomly divided into 4 groups (n = 6 for each group): sham operation, torsion/detorsion (T/D), T/D + vehicle, and T/D + DHEA. Three hours before detorsion, 50 mg/kg DHEA was given intraperitoneally to the T/D + DHEA group. Testicular ischemia was achieved by twisting the left testis 720 degrees clockwise for 3 hours, and reperfusion was allowed for 24 hours after detorsion. In all groups, bilateral orchiectomies to determine the testicular tissue catalase (CAT) and superoxide dismutase activities and malondialdehyde (MDA) levels and histopathologic examination were performed.

RESULTS

Compared with those from the sham group, CAT activities in the ipsilateral testis obtained from the T/D group were significantly lower and MDA levels were significantly higher (P < .05 for all). Administration of DHEA prevented increases in MDA levels and decreases in CAT and superoxide dismutase activities when compared to the T/D group. Specimens from the T/D and the T/D + vehicle groups had a significantly greater histologic injury than the specimens from the sham and the T/D + DHEA groups had (P < .01 for both).

CONCLUSIONS

The results suggest that DHEA may be a protective agent for preventing biochemical and histopathologic changes related to oxidative stress in testicular injury caused by testis torsion.

Authors+Show Affiliations

Department of Biochemistry, School of Medicine, Ataturk University, TR-25240 Erzurum, Turkey. aksoyhulya@yahoo.comNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Journal Article

Language

eng

PubMed ID

17923206

Citation

Aksoy, Hülya, et al. "Dehydroepiandrosterone Treatment Attenuates Reperfusion Injury After Testicular Torsion and Detorsion in Rats." Journal of Pediatric Surgery, vol. 42, no. 10, 2007, pp. 1740-4.
Aksoy H, Yapanoglu T, Aksoy Y, et al. Dehydroepiandrosterone treatment attenuates reperfusion injury after testicular torsion and detorsion in rats. J Pediatr Surg. 2007;42(10):1740-4.
Aksoy, H., Yapanoglu, T., Aksoy, Y., Ozbey, I., Turhan, H., & Gursan, N. (2007). Dehydroepiandrosterone treatment attenuates reperfusion injury after testicular torsion and detorsion in rats. Journal of Pediatric Surgery, 42(10), 1740-4.
Aksoy H, et al. Dehydroepiandrosterone Treatment Attenuates Reperfusion Injury After Testicular Torsion and Detorsion in Rats. J Pediatr Surg. 2007;42(10):1740-4. PubMed PMID: 17923206.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Dehydroepiandrosterone treatment attenuates reperfusion injury after testicular torsion and detorsion in rats. AU - Aksoy,Hülya, AU - Yapanoglu,Turgut, AU - Aksoy,Yilmaz, AU - Ozbey,Isa, AU - Turhan,Hamdullah, AU - Gursan,Nesrin, PY - 2007/10/10/pubmed PY - 2007/10/25/medline PY - 2007/10/10/entrez SP - 1740 EP - 4 JF - Journal of pediatric surgery JO - J Pediatr Surg VL - 42 IS - 10 N2 - PURPOSE: We aimed to evaluate the effects of dehydroepiandrosterone (DHEA) on antioxidant enzyme activities, lipid peroxidation, and histopathologic changes in both testes after unilateral testicular torsion and detorsion. METHODS: Twenty-four adult male Sprague-Dawley rats were randomly divided into 4 groups (n = 6 for each group): sham operation, torsion/detorsion (T/D), T/D + vehicle, and T/D + DHEA. Three hours before detorsion, 50 mg/kg DHEA was given intraperitoneally to the T/D + DHEA group. Testicular ischemia was achieved by twisting the left testis 720 degrees clockwise for 3 hours, and reperfusion was allowed for 24 hours after detorsion. In all groups, bilateral orchiectomies to determine the testicular tissue catalase (CAT) and superoxide dismutase activities and malondialdehyde (MDA) levels and histopathologic examination were performed. RESULTS: Compared with those from the sham group, CAT activities in the ipsilateral testis obtained from the T/D group were significantly lower and MDA levels were significantly higher (P < .05 for all). Administration of DHEA prevented increases in MDA levels and decreases in CAT and superoxide dismutase activities when compared to the T/D group. Specimens from the T/D and the T/D + vehicle groups had a significantly greater histologic injury than the specimens from the sham and the T/D + DHEA groups had (P < .01 for both). CONCLUSIONS: The results suggest that DHEA may be a protective agent for preventing biochemical and histopathologic changes related to oxidative stress in testicular injury caused by testis torsion. SN - 1531-5037 UR - https://www.unboundmedicine.com/medline/citation/17923206/Dehydroepiandrosterone_treatment_attenuates_reperfusion_injury_after_testicular_torsion_and_detorsion_in_rats_ DB - PRIME DP - Unbound Medicine ER -