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Oxidative stress in non-insulin-dependent diabetes mellitus (NIDDM) patients.
Acta Diabetol. 2008 Mar; 45(1):41-6.AD

Abstract

Diabetes mellitus is a metabolic disorder characterized by hyperglycemia. The oxidative stress in diabetes was greatly increased due to prolonged exposure to hyperglycemia and impairment of oxidant/antioxidant equilibrium. Proteins and lipids are among the prime targets for oxidative stress. In the present study, the oxidative stress was evaluated in 55 diabetic patients and 40 healthy subjects by measuring the levels of protein oxidation, lipid peroxidation and some enzymatic and nonenzymatic antioxidants. The oxidative products of protein (PCG) and lipid peroxidation (MDA) and nitric oxide levels in plasma of NIDDM patients were significantly increased. However, the levels of enzymatic (GPx, SOD, catalase in RBC) and nonenzymatic (beta-carotene, retinol, vitamin C & E and uric acid) antioxidants of RBC showed a significant decrease in NIDDM patients compared to normal subjects. Serum protein analysis by polyacrylamide gel electrophoresis (PAGE) showed the significant difference in the ceruloplasmin, transferrin, albumin, retinal binding protein, etc. in diabetic patients compared to healthy controls. In conclusion, the results suggest that increased protein oxidation, lipid peroxidation and NO levels, decreases the levels of enzymatic and nonenzymatic antioxidants and playing a major role in diabetic complications.

Authors+Show Affiliations

Department of Biotechnology, Sri Krishnadevaraya University, Anantapur, 515003, India. vrkrishna70@rediffmail.comNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

17924055

Citation

Ramakrishna, Vadde, and Rama Jailkhani. "Oxidative Stress in Non-insulin-dependent Diabetes Mellitus (NIDDM) Patients." Acta Diabetologica, vol. 45, no. 1, 2008, pp. 41-6.
Ramakrishna V, Jailkhani R. Oxidative stress in non-insulin-dependent diabetes mellitus (NIDDM) patients. Acta Diabetol. 2008;45(1):41-6.
Ramakrishna, V., & Jailkhani, R. (2008). Oxidative stress in non-insulin-dependent diabetes mellitus (NIDDM) patients. Acta Diabetologica, 45(1), 41-6.
Ramakrishna V, Jailkhani R. Oxidative Stress in Non-insulin-dependent Diabetes Mellitus (NIDDM) Patients. Acta Diabetol. 2008;45(1):41-6. PubMed PMID: 17924055.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Oxidative stress in non-insulin-dependent diabetes mellitus (NIDDM) patients. AU - Ramakrishna,Vadde, AU - Jailkhani,Rama, Y1 - 2007/10/09/ PY - 2006/06/09/received PY - 2007/09/12/accepted PY - 2007/10/10/pubmed PY - 2008/8/12/medline PY - 2007/10/10/entrez SP - 41 EP - 6 JF - Acta diabetologica JO - Acta Diabetol VL - 45 IS - 1 N2 - Diabetes mellitus is a metabolic disorder characterized by hyperglycemia. The oxidative stress in diabetes was greatly increased due to prolonged exposure to hyperglycemia and impairment of oxidant/antioxidant equilibrium. Proteins and lipids are among the prime targets for oxidative stress. In the present study, the oxidative stress was evaluated in 55 diabetic patients and 40 healthy subjects by measuring the levels of protein oxidation, lipid peroxidation and some enzymatic and nonenzymatic antioxidants. The oxidative products of protein (PCG) and lipid peroxidation (MDA) and nitric oxide levels in plasma of NIDDM patients were significantly increased. However, the levels of enzymatic (GPx, SOD, catalase in RBC) and nonenzymatic (beta-carotene, retinol, vitamin C & E and uric acid) antioxidants of RBC showed a significant decrease in NIDDM patients compared to normal subjects. Serum protein analysis by polyacrylamide gel electrophoresis (PAGE) showed the significant difference in the ceruloplasmin, transferrin, albumin, retinal binding protein, etc. in diabetic patients compared to healthy controls. In conclusion, the results suggest that increased protein oxidation, lipid peroxidation and NO levels, decreases the levels of enzymatic and nonenzymatic antioxidants and playing a major role in diabetic complications. SN - 0940-5429 UR - https://www.unboundmedicine.com/medline/citation/17924055/Oxidative_stress_in_non_insulin_dependent_diabetes_mellitus__NIDDM__patients_ L2 - https://dx.doi.org/10.1007/s00592-007-0018-3 DB - PRIME DP - Unbound Medicine ER -