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Oxidative stress response in eukaryotes: effect of glutathione, superoxide dismutase and catalase on adaptation to peroxide and menadione stresses in Saccharomyces cerevisiae.
Redox Rep. 2007; 12(5):236-44.RR

Abstract

Aiming to clarify the mechanisms by which eukaryotes acquire tolerance to oxidative stress, adaptive and cross-protection responses to oxidants were investigated in Saccharomyces cerevisiae. Cells treated with sub-lethal concentrations of menadione (a source of superoxide anions) exhibited cross-protection against lethal doses of peroxide; however, cells treated with H2O2 did not acquire tolerance to a menadione stress, indicating that menadione response encompasses H2O2 adaptation. Although, deficiency in cytoplasmic superoxide dismutase (Sod1) had not interfered with response to superoxide, cells deficient in glutathione (GSH) synthesis were not able to acquire tolerance to H2O2 when pretreated with menadione. These results suggest that GSH is an inducible part of the superoxide adaptive stress response, which correlates with a decrease in the levels of intracellular oxidation. On the other hand, neither the deficiency of Sod1 nor in GSH impaired the process of acquisition of tolerance to H2O2 achieved by a mild pretreatment with peroxide. Using a strain deficient in the cytosolic catalase, we were able to conclude that the reduction in lipid peroxidation levels produced by the adaptive treatment with H2O2 was dependent on this enzyme. Corroborating these results, the pretreatment with low concentrations of H2O2 promoted an increase in catalase activity.

Authors+Show Affiliations

Departamento de Bioquímica, Instituto de Química, UFRJ, Rio de Janeiro, Brazil.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

17925096

Citation

Fernandes, Patricia N., et al. "Oxidative Stress Response in Eukaryotes: Effect of Glutathione, Superoxide Dismutase and Catalase On Adaptation to Peroxide and Menadione Stresses in Saccharomyces Cerevisiae." Redox Report : Communications in Free Radical Research, vol. 12, no. 5, 2007, pp. 236-44.
Fernandes PN, Mannarino SC, Silva CG, et al. Oxidative stress response in eukaryotes: effect of glutathione, superoxide dismutase and catalase on adaptation to peroxide and menadione stresses in Saccharomyces cerevisiae. Redox Rep. 2007;12(5):236-44.
Fernandes, P. N., Mannarino, S. C., Silva, C. G., Pereira, M. D., Panek, A. D., & Eleutherio, E. C. (2007). Oxidative stress response in eukaryotes: effect of glutathione, superoxide dismutase and catalase on adaptation to peroxide and menadione stresses in Saccharomyces cerevisiae. Redox Report : Communications in Free Radical Research, 12(5), 236-44.
Fernandes PN, et al. Oxidative Stress Response in Eukaryotes: Effect of Glutathione, Superoxide Dismutase and Catalase On Adaptation to Peroxide and Menadione Stresses in Saccharomyces Cerevisiae. Redox Rep. 2007;12(5):236-44. PubMed PMID: 17925096.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Oxidative stress response in eukaryotes: effect of glutathione, superoxide dismutase and catalase on adaptation to peroxide and menadione stresses in Saccharomyces cerevisiae. AU - Fernandes,Patricia N, AU - Mannarino,Sergio C, AU - Silva,Carmelita G, AU - Pereira,Marcos D, AU - Panek,Anita D, AU - Eleutherio,Elis C A, PY - 2007/10/11/pubmed PY - 2007/12/14/medline PY - 2007/10/11/entrez SP - 236 EP - 44 JF - Redox report : communications in free radical research JO - Redox Rep VL - 12 IS - 5 N2 - Aiming to clarify the mechanisms by which eukaryotes acquire tolerance to oxidative stress, adaptive and cross-protection responses to oxidants were investigated in Saccharomyces cerevisiae. Cells treated with sub-lethal concentrations of menadione (a source of superoxide anions) exhibited cross-protection against lethal doses of peroxide; however, cells treated with H2O2 did not acquire tolerance to a menadione stress, indicating that menadione response encompasses H2O2 adaptation. Although, deficiency in cytoplasmic superoxide dismutase (Sod1) had not interfered with response to superoxide, cells deficient in glutathione (GSH) synthesis were not able to acquire tolerance to H2O2 when pretreated with menadione. These results suggest that GSH is an inducible part of the superoxide adaptive stress response, which correlates with a decrease in the levels of intracellular oxidation. On the other hand, neither the deficiency of Sod1 nor in GSH impaired the process of acquisition of tolerance to H2O2 achieved by a mild pretreatment with peroxide. Using a strain deficient in the cytosolic catalase, we were able to conclude that the reduction in lipid peroxidation levels produced by the adaptive treatment with H2O2 was dependent on this enzyme. Corroborating these results, the pretreatment with low concentrations of H2O2 promoted an increase in catalase activity. SN - 1743-2928 UR - https://www.unboundmedicine.com/medline/citation/17925096/Oxidative_stress_response_in_eukaryotes:_effect_of_glutathione_superoxide_dismutase_and_catalase_on_adaptation_to_peroxide_and_menadione_stresses_in_Saccharomyces_cerevisiae_ L2 - https://www.tandfonline.com/doi/full/10.1179/135100007X200344 DB - PRIME DP - Unbound Medicine ER -