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Carbohydrate-binding agents (CBAs) inhibit HIV-1 infection in human primary monocyte-derived macrophages (MDMs) and efficiently prevent MDM-directed viral capture and subsequent transmission to CD4+ T lymphocytes.
Virology. 2008 Jan 20; 370(2):382-91.V

Abstract

Carbohydrate-binding agents (CBAs) have been proposed as innovative anti-HIV compounds selectively targeting the glycans of the HIV-1 envelope glycoprotein gp120 and preventing DC-SIGN-directed HIV capture by dendritic cells (DCs) and transmission to CD4(+) T-lymphocytes. We now show that CBAs efficiently prevent R5 HIV-1 infection of human primary monocyte-derived macrophage (MDM) cell cultures in the nanomolar range. Both R5 and X4 HIV-1 strains were efficiently captured by the macrophage mannose-binding receptor (MMR) present on MDM. HIV-1 capture by MMR-expressing MDM was inhibited by soluble mannose-binding lectin and MMR antibody. Short pre-exposure of these HIV-1 strains to CBAs is able to prevent virus capture by MDM and subsequent syncytia formation in cocultures of the CBA-exposed HIV-1-captured MDM and uninfected CD4(+) T-lymphocytes. The potential of CBAs to impair MDM in their capacity to capture and to transmit HIV to T-lymphocytes might be an important property to be taken into consideration in the eventual choice to select microbicide candidate drugs for clinical investigation.

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Authors+Show Affiliations

Pollicita M
University of Rome, Tor Vergata, Italy.
Schols D
No affiliation info available
Aquaro S
No affiliation info available
Peumans WJ
No affiliation info available
Van Damme EJ
No affiliation info available
Perno CF
No affiliation info available
Balzarini J
No affiliation info available

MeSH

Anti-HIV AgentsCD4-Positive T-LymphocytesCarbohydrate MetabolismCell Adhesion MoleculesCells, CulturedDrug Evaluation, PreclinicalHIV-1HumansLectins, C-TypeMacrophagesMannose-Binding LectinsMicrobial Sensitivity TestsPlant LectinsReceptors, Cell Surface

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

17928023

Citation

Pollicita, M, et al. "Carbohydrate-binding Agents (CBAs) Inhibit HIV-1 Infection in Human Primary Monocyte-derived Macrophages (MDMs) and Efficiently Prevent MDM-directed Viral Capture and Subsequent Transmission to CD4+ T Lymphocytes." Virology, vol. 370, no. 2, 2008, pp. 382-91.
Pollicita M, Schols D, Aquaro S, et al. Carbohydrate-binding agents (CBAs) inhibit HIV-1 infection in human primary monocyte-derived macrophages (MDMs) and efficiently prevent MDM-directed viral capture and subsequent transmission to CD4+ T lymphocytes. Virology. 2008;370(2):382-91.
Pollicita, M., Schols, D., Aquaro, S., Peumans, W. J., Van Damme, E. J., Perno, C. F., & Balzarini, J. (2008). Carbohydrate-binding agents (CBAs) inhibit HIV-1 infection in human primary monocyte-derived macrophages (MDMs) and efficiently prevent MDM-directed viral capture and subsequent transmission to CD4+ T lymphocytes. Virology, 370(2), 382-91.
Pollicita M, et al. Carbohydrate-binding Agents (CBAs) Inhibit HIV-1 Infection in Human Primary Monocyte-derived Macrophages (MDMs) and Efficiently Prevent MDM-directed Viral Capture and Subsequent Transmission to CD4+ T Lymphocytes. Virology. 2008 Jan 20;370(2):382-91. PubMed PMID: 17928023.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Carbohydrate-binding agents (CBAs) inhibit HIV-1 infection in human primary monocyte-derived macrophages (MDMs) and efficiently prevent MDM-directed viral capture and subsequent transmission to CD4+ T lymphocytes. AU - Pollicita,M, AU - Schols,D, AU - Aquaro,S, AU - Peumans,W J, AU - Van Damme,E J M, AU - Perno,C F, AU - Balzarini,J, Y1 - 2007/10/24/ PY - 2007/07/18/received PY - 2007/08/20/revised PY - 2007/08/30/accepted PY - 2007/10/12/pubmed PY - 2008/2/21/medline PY - 2007/10/12/entrez SP - 382 EP - 91 JF - Virology JO - Virology VL - 370 IS - 2 N2 - Carbohydrate-binding agents (CBAs) have been proposed as innovative anti-HIV compounds selectively targeting the glycans of the HIV-1 envelope glycoprotein gp120 and preventing DC-SIGN-directed HIV capture by dendritic cells (DCs) and transmission to CD4(+) T-lymphocytes. We now show that CBAs efficiently prevent R5 HIV-1 infection of human primary monocyte-derived macrophage (MDM) cell cultures in the nanomolar range. Both R5 and X4 HIV-1 strains were efficiently captured by the macrophage mannose-binding receptor (MMR) present on MDM. HIV-1 capture by MMR-expressing MDM was inhibited by soluble mannose-binding lectin and MMR antibody. Short pre-exposure of these HIV-1 strains to CBAs is able to prevent virus capture by MDM and subsequent syncytia formation in cocultures of the CBA-exposed HIV-1-captured MDM and uninfected CD4(+) T-lymphocytes. The potential of CBAs to impair MDM in their capacity to capture and to transmit HIV to T-lymphocytes might be an important property to be taken into consideration in the eventual choice to select microbicide candidate drugs for clinical investigation. SN - 0042-6822 UR - https://www.unboundmedicine.com/medline/citation/17928023/Carbohydrate_binding_agents__CBAs__inhibit_HIV_1_infection_in_human_primary_monocyte_derived_macrophages__MDMs__and_efficiently_prevent_MDM_directed_viral_capture_and_subsequent_transmission_to_CD4+_T_lymphocytes_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0042-6822(07)00571-5 DB - PRIME DP - Unbound Medicine ER -