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Outbreak of carbapenem-resistant Klebsiella pneumoniae producing KPC-3 in a tertiary medical centre in Israel.
Int J Antimicrob Agents. 2007 Dec; 30(6):525-9.IJ

Abstract

This report describes an outbreak of carbapenem-resistant KPC-3-producing Klebsiella pneumoniae outside the USA. Ninety patients from different departments of a tertiary medical centre were diagnosed with carbapenem-resistant, extended-spectrum beta-lactamase (ESBL)-negative Klebsiella pneumoniae infection by standard methods over a 10-month period in 2006. Fifteen randomly selected outbreak isolates were subjected to randomly amplified polymorphic DNA (RAPD) polymerase chain reaction (PCR) as well as PCR amplification and sequencing of the KPC genes, and the findings were compared with two carbapenem-susceptible K. pneumoniae isolates (one ESBL-positive and one ESBL-negative). All the outbreak isolates were resistant to all fluoroquinolones and beta-lactam antibiotics tested, including carbapenems, and were sensitive only to colistin, gentamicin and most of them also to tigecycline. On RAPD-PCR, all 15 outbreak isolates were identical to each other and clearly distinguishable from control strains, indicating clonality. The KPC-3 enzyme was identified by nucleotide sequencing analysis in all outbreak isolates but not in the control strains. These findings should alert government and medical authorities to institute stringent control measures and to initiate research into therapeutic and preventive strategies.

Authors+Show Affiliations

Laboratory of Clinical Microbiology, Rabin Medical Center, Beilinson Hospital, Petah Tiqwa 49100, Sackler Faculty of Medicine, Tel Aviv University, Tel Aviv, Israel.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

17931835

Citation

Samra, Zmira, et al. "Outbreak of Carbapenem-resistant Klebsiella Pneumoniae Producing KPC-3 in a Tertiary Medical Centre in Israel." International Journal of Antimicrobial Agents, vol. 30, no. 6, 2007, pp. 525-9.
Samra Z, Ofir O, Lishtzinsky Y, et al. Outbreak of carbapenem-resistant Klebsiella pneumoniae producing KPC-3 in a tertiary medical centre in Israel. Int J Antimicrob Agents. 2007;30(6):525-9.
Samra, Z., Ofir, O., Lishtzinsky, Y., Madar-Shapiro, L., & Bishara, J. (2007). Outbreak of carbapenem-resistant Klebsiella pneumoniae producing KPC-3 in a tertiary medical centre in Israel. International Journal of Antimicrobial Agents, 30(6), 525-9.
Samra Z, et al. Outbreak of Carbapenem-resistant Klebsiella Pneumoniae Producing KPC-3 in a Tertiary Medical Centre in Israel. Int J Antimicrob Agents. 2007;30(6):525-9. PubMed PMID: 17931835.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Outbreak of carbapenem-resistant Klebsiella pneumoniae producing KPC-3 in a tertiary medical centre in Israel. AU - Samra,Zmira, AU - Ofir,Orit, AU - Lishtzinsky,Yinon, AU - Madar-Shapiro,Liora, AU - Bishara,Jihad, Y1 - 2007/10/10/ PY - 2007/05/04/received PY - 2007/07/31/revised PY - 2007/07/31/accepted PY - 2007/10/13/pubmed PY - 2008/1/30/medline PY - 2007/10/13/entrez SP - 525 EP - 9 JF - International journal of antimicrobial agents JO - Int J Antimicrob Agents VL - 30 IS - 6 N2 - This report describes an outbreak of carbapenem-resistant KPC-3-producing Klebsiella pneumoniae outside the USA. Ninety patients from different departments of a tertiary medical centre were diagnosed with carbapenem-resistant, extended-spectrum beta-lactamase (ESBL)-negative Klebsiella pneumoniae infection by standard methods over a 10-month period in 2006. Fifteen randomly selected outbreak isolates were subjected to randomly amplified polymorphic DNA (RAPD) polymerase chain reaction (PCR) as well as PCR amplification and sequencing of the KPC genes, and the findings were compared with two carbapenem-susceptible K. pneumoniae isolates (one ESBL-positive and one ESBL-negative). All the outbreak isolates were resistant to all fluoroquinolones and beta-lactam antibiotics tested, including carbapenems, and were sensitive only to colistin, gentamicin and most of them also to tigecycline. On RAPD-PCR, all 15 outbreak isolates were identical to each other and clearly distinguishable from control strains, indicating clonality. The KPC-3 enzyme was identified by nucleotide sequencing analysis in all outbreak isolates but not in the control strains. These findings should alert government and medical authorities to institute stringent control measures and to initiate research into therapeutic and preventive strategies. SN - 0924-8579 UR - https://www.unboundmedicine.com/medline/citation/17931835/Outbreak_of_carbapenem_resistant_Klebsiella_pneumoniae_producing_KPC_3_in_a_tertiary_medical_centre_in_Israel_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0924-8579(07)00374-3 DB - PRIME DP - Unbound Medicine ER -