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Crystal structure of the MarR family regulatory protein, ST1710, from Sulfolobus tokodaii strain 7.
J Struct Biol. 2008 Jan; 161(1):9-17.JS

Abstract

The emergence of bacterial resistance to multiple drugs poses a serious and growing health concern. Understanding and deciphering the mechanisms of these multiple drug resistance regulatory proteins through structural or biochemical means is an important endeavor. Here, we present the crystal structure of ST1710 from Sulfolobus tokodaii strain 7 in two different crystal forms, at 1.80 and 2.0A, respectively. The overall structure of the ST1710 dimer shares the topology of the MarR family of proteins, with each subunit containing a winged helix-turn-helix DNA-binding motif. We also show the protein-DNA interactions by biochemical methods. Our molecular modeling analysis suggested that Asp88 and Arg90 are the key residues in ST1710 involved in the protein-DNA interactions.

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Authors+Show Affiliations

Kumarevel T
RIKEN SPring-8 Center, Harima Institute, 1-1-1 Kouto, Sayo, Hyogo 679-5148, Japan. tskvel@spring8.or.jp
Tanaka T
No affiliation info available
Nishio M
No affiliation info available
Gopinath SC
No affiliation info available
Takio K
No affiliation info available
Shinkai A
No affiliation info available
Kumar PK
No affiliation info available
Yokoyama S
No affiliation info available

MeSH

Amino Acid SequenceArchaeal ProteinsCrystallizationCrystallography, X-RayDNA-Binding ProteinsElectrophoretic Mobility Shift AssayHelix-Turn-Helix MotifsMolecular Sequence DataProtein BindingProtein Structure, SecondaryProtein Structure, TertiarySequence Homology, Amino AcidStructure-Activity RelationshipSulfolobus

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

17933554

Citation

Kumarevel, Thirumananseri, et al. "Crystal Structure of the MarR Family Regulatory Protein, ST1710, From Sulfolobus Tokodaii Strain 7." Journal of Structural Biology, vol. 161, no. 1, 2008, pp. 9-17.
Kumarevel T, Tanaka T, Nishio M, et al. Crystal structure of the MarR family regulatory protein, ST1710, from Sulfolobus tokodaii strain 7. J Struct Biol. 2008;161(1):9-17.
Kumarevel, T., Tanaka, T., Nishio, M., Gopinath, S. C., Takio, K., Shinkai, A., Kumar, P. K., & Yokoyama, S. (2008). Crystal structure of the MarR family regulatory protein, ST1710, from Sulfolobus tokodaii strain 7. Journal of Structural Biology, 161(1), 9-17.
Kumarevel T, et al. Crystal Structure of the MarR Family Regulatory Protein, ST1710, From Sulfolobus Tokodaii Strain 7. J Struct Biol. 2008;161(1):9-17. PubMed PMID: 17933554.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Crystal structure of the MarR family regulatory protein, ST1710, from Sulfolobus tokodaii strain 7. AU - Kumarevel,Thirumananseri, AU - Tanaka,Tomoyuki, AU - Nishio,Megumi, AU - Gopinath,Subash C B, AU - Takio,Koji, AU - Shinkai,Akeo, AU - Kumar,Penmetcha K R, AU - Yokoyama,Shigeyuki, Y1 - 2007/09/08/ PY - 2007/05/21/received PY - 2007/08/24/revised PY - 2007/08/28/accepted PY - 2007/10/16/pubmed PY - 2008/5/31/medline PY - 2007/10/16/entrez SP - 9 EP - 17 JF - Journal of structural biology JO - J Struct Biol VL - 161 IS - 1 N2 - The emergence of bacterial resistance to multiple drugs poses a serious and growing health concern. Understanding and deciphering the mechanisms of these multiple drug resistance regulatory proteins through structural or biochemical means is an important endeavor. Here, we present the crystal structure of ST1710 from Sulfolobus tokodaii strain 7 in two different crystal forms, at 1.80 and 2.0A, respectively. The overall structure of the ST1710 dimer shares the topology of the MarR family of proteins, with each subunit containing a winged helix-turn-helix DNA-binding motif. We also show the protein-DNA interactions by biochemical methods. Our molecular modeling analysis suggested that Asp88 and Arg90 are the key residues in ST1710 involved in the protein-DNA interactions. SN - 1047-8477 UR - https://www.unboundmedicine.com/medline/citation/17933554/Crystal_structure_of_the_MarR_family_regulatory_protein_ST1710_from_Sulfolobus_tokodaii_strain_7_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S1047-8477(07)00205-5 DB - PRIME DP - Unbound Medicine ER -