Tags

Type your tag names separated by a space and hit enter

Radical scavenging activities of Rio Red grapefruits and Sour orange fruit extracts in different in vitro model systems.

Abstract

Antioxidant fractions from two different citrus species such as Rio Red (Citrus paradise Macf.) and Sour orange (Citrus aurantium L.) were extracted with five different polar solvents using Soxhlet type extractor. The total phenolic content of the extracts was determined by Folin-Ciocalteu method. Ethyl acetate extract of Rio Red and Sour orange was found to contain maximum phenolics. The dried fractions were screened for their antioxidant activity potential using in vitro model systems such as 1,1-diphenyl-2-picryl hydrazyl (DPPH), phosphomolybdenum method and nitroblue tetrazolium (NBT) reduction at different concentrations. The methanol:water (80:20) fraction of Rio Red showed the highest radical scavenging activity 42.5%, 77.8% and 92.1% at 250, 500 and 1000 ppm, respectively, while methanol:water (80:20) fraction of Sour orange showed the lowest radical scavenging activity at all the tested concentrations. All citrus fractions showed good antioxidant capacity by the formation of phosphomolybdenum complex at 200 ppm. In addition, superoxide radical scavenging activity was assayed using non-enzymatic (NADH/phenaxine methosulfate) superoxide generating system. All the extracts showed variable superoxide radical scavenging activity. Moreover, methanol:water (80:20) extract of Rio Red and methanol extract of Sour orange exhibited marked reducing power in potassium ferricyanide reduction method. The data obtained using above in vitro models clearly establish the antioxidant potential of citrus fruit extracts. However, comprehensive studies need to be conducted to ascertain the in vivo bioavailability, safety and efficacy of such extracts in experimental animals. To the best of our knowledge, this is the first report on antioxidant activity of different polar extracts from Rio Red and Sour oranges.

Links

  • Publisher Full Text
  • Authors+Show Affiliations

    ,

    Vegetable and Fruit Improvement Center, Department of Horticultural Sciences, Texas A&M University, 1500 Research Parkway, Suite 120A, College Station, TX 77845, USA.

    ,

    Source

    Bioresource technology 99:10 2008 Jul pg 4484-94

    MeSH

    Antioxidants
    Biotechnology
    Chromatography, High Pressure Liquid
    Citrus
    Citrus paradisi
    Citrus sinensis
    Ferricyanides
    Free Radical Scavengers
    Fruit
    In Vitro Techniques
    Molybdenum
    NAD
    Nitroblue Tetrazolium
    Phenol
    Phosphoric Acids
    Plants
    Superoxides

    Pub Type(s)

    Journal Article
    Research Support, Non-U.S. Gov't

    Language

    eng

    PubMed ID

    17935981

    Citation

    Jayaprakasha, G K., et al. "Radical Scavenging Activities of Rio Red Grapefruits and Sour Orange Fruit Extracts in Different in Vitro Model Systems." Bioresource Technology, vol. 99, no. 10, 2008, pp. 4484-94.
    Jayaprakasha GK, Girennavar B, Patil BS. Radical scavenging activities of Rio Red grapefruits and Sour orange fruit extracts in different in vitro model systems. Bioresour Technol. 2008;99(10):4484-94.
    Jayaprakasha, G. K., Girennavar, B., & Patil, B. S. (2008). Radical scavenging activities of Rio Red grapefruits and Sour orange fruit extracts in different in vitro model systems. Bioresource Technology, 99(10), pp. 4484-94.
    Jayaprakasha GK, Girennavar B, Patil BS. Radical Scavenging Activities of Rio Red Grapefruits and Sour Orange Fruit Extracts in Different in Vitro Model Systems. Bioresour Technol. 2008;99(10):4484-94. PubMed PMID: 17935981.
    * Article titles in AMA citation format should be in sentence-case
    TY - JOUR T1 - Radical scavenging activities of Rio Red grapefruits and Sour orange fruit extracts in different in vitro model systems. AU - Jayaprakasha,G K, AU - Girennavar,Basavaraj, AU - Patil,Bhimanagouda S, Y1 - 2007/11/01/ PY - 2006/05/10/received PY - 2007/07/25/revised PY - 2007/07/25/accepted PY - 2007/10/16/pubmed PY - 2008/6/17/medline PY - 2007/10/16/entrez SP - 4484 EP - 94 JF - Bioresource technology JO - Bioresour. Technol. VL - 99 IS - 10 N2 - Antioxidant fractions from two different citrus species such as Rio Red (Citrus paradise Macf.) and Sour orange (Citrus aurantium L.) were extracted with five different polar solvents using Soxhlet type extractor. The total phenolic content of the extracts was determined by Folin-Ciocalteu method. Ethyl acetate extract of Rio Red and Sour orange was found to contain maximum phenolics. The dried fractions were screened for their antioxidant activity potential using in vitro model systems such as 1,1-diphenyl-2-picryl hydrazyl (DPPH), phosphomolybdenum method and nitroblue tetrazolium (NBT) reduction at different concentrations. The methanol:water (80:20) fraction of Rio Red showed the highest radical scavenging activity 42.5%, 77.8% and 92.1% at 250, 500 and 1000 ppm, respectively, while methanol:water (80:20) fraction of Sour orange showed the lowest radical scavenging activity at all the tested concentrations. All citrus fractions showed good antioxidant capacity by the formation of phosphomolybdenum complex at 200 ppm. In addition, superoxide radical scavenging activity was assayed using non-enzymatic (NADH/phenaxine methosulfate) superoxide generating system. All the extracts showed variable superoxide radical scavenging activity. Moreover, methanol:water (80:20) extract of Rio Red and methanol extract of Sour orange exhibited marked reducing power in potassium ferricyanide reduction method. The data obtained using above in vitro models clearly establish the antioxidant potential of citrus fruit extracts. However, comprehensive studies need to be conducted to ascertain the in vivo bioavailability, safety and efficacy of such extracts in experimental animals. To the best of our knowledge, this is the first report on antioxidant activity of different polar extracts from Rio Red and Sour oranges. SN - 0960-8524 UR - https://www.unboundmedicine.com/medline/citation/17935981/Radical_scavenging_activities_of_Rio_Red_grapefruits_and_Sour_orange_fruit_extracts_in_different_in_vitro_model_systems_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0960-8524(07)00735-3 DB - PRIME DP - Unbound Medicine ER -