Tags

Type your tag names separated by a space and hit enter

Food deprivation induces adipose plasminogen activator inhibitor-1 (PAI-1) expression without accumulation of plasma PAI-1 in genetically obese and diabetic db/db mice.
Thromb Haemost 2007; 98(4):864-70TH

Abstract

Relationships between energy intake and fibrinolytic functions have been documented in detail. We evaluated food deprivation (FD) as a means of modulating fibrinolytic activity in genetically obese and diabetic db/db mice and in their lean counterparts. Twelve hours of FD induced considerable gene expression of plasminogen activator inhibitor-1 (PAI-1) in both epididymal (3.8-fold, p < 0.05) and intestinal (2.4-fold, p < 0.05) adipose tissues without affecting plasma PAI-1 levels in db/db mice, whereas the FD did not affect these parameters in wild-type mice. Importantly, 24 hours of FD increased the plasma PAI-1 content in wild-type (1.9-fold, p < 0.01) but not in db/db mice, although adipose PAI-1 mRNA levels were significantly increased in db/db mice. The plasma PAI-1 content significantly correlated with hepatic PAI-1 mRNA levels in wild-type (r = 0.84, p < 0.01) and in db/db (r = 0.63, p < 0.01) mice. However, plasma PAI-1 did not correlate with adipose PAI-1 expression in db/db mice, although adipose tissue in general is thought to be the principal site of PAI-1 production in obesity. Hepatic PAI-1 expression was closely correlated with serum levels of free fatty acids in wild-type (r = 0.72, p < 0.01), but not in db/db mice. Adipose PAI-1 expression significantly correlated with serum corticosterone levels in both genotypes (wild-type, r = 0.52, p < 0.05; db/db, r = 0.51, p < 0.01), suggesting that adipose PAI-1 expression is up-regulated by fasting-induced glucocorticoids. The present findings suggested that fasting differentially affects fibrinolytic activity in obese and lean subjects and that PAI-1 expression in the liver as well as in adipose tissues comprises an important determinant of increased risk for cardiovascular disease in obesity.

Authors+Show Affiliations

Clock Cell Biology Research Group, National Institute of Advanced Industrial Science and Technology, Central 6, 1-1-1, Higashi, Tsukuba, Ibaraki 305-8566, Japan.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

17938813

Citation

Oishi, Katsutaka, et al. "Food Deprivation Induces Adipose Plasminogen Activator Inhibitor-1 (PAI-1) Expression Without Accumulation of Plasma PAI-1 in Genetically Obese and Diabetic Db/db Mice." Thrombosis and Haemostasis, vol. 98, no. 4, 2007, pp. 864-70.
Oishi K, Ohkura N, Matsuda J, et al. Food deprivation induces adipose plasminogen activator inhibitor-1 (PAI-1) expression without accumulation of plasma PAI-1 in genetically obese and diabetic db/db mice. Thromb Haemost. 2007;98(4):864-70.
Oishi, K., Ohkura, N., Matsuda, J., & Ishida, N. (2007). Food deprivation induces adipose plasminogen activator inhibitor-1 (PAI-1) expression without accumulation of plasma PAI-1 in genetically obese and diabetic db/db mice. Thrombosis and Haemostasis, 98(4), pp. 864-70.
Oishi K, et al. Food Deprivation Induces Adipose Plasminogen Activator Inhibitor-1 (PAI-1) Expression Without Accumulation of Plasma PAI-1 in Genetically Obese and Diabetic Db/db Mice. Thromb Haemost. 2007;98(4):864-70. PubMed PMID: 17938813.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Food deprivation induces adipose plasminogen activator inhibitor-1 (PAI-1) expression without accumulation of plasma PAI-1 in genetically obese and diabetic db/db mice. AU - Oishi,Katsutaka, AU - Ohkura,Naoki, AU - Matsuda,Juzo, AU - Ishida,Norio, PY - 2007/10/17/pubmed PY - 2008/1/15/medline PY - 2007/10/17/entrez SP - 864 EP - 70 JF - Thrombosis and haemostasis JO - Thromb. Haemost. VL - 98 IS - 4 N2 - Relationships between energy intake and fibrinolytic functions have been documented in detail. We evaluated food deprivation (FD) as a means of modulating fibrinolytic activity in genetically obese and diabetic db/db mice and in their lean counterparts. Twelve hours of FD induced considerable gene expression of plasminogen activator inhibitor-1 (PAI-1) in both epididymal (3.8-fold, p < 0.05) and intestinal (2.4-fold, p < 0.05) adipose tissues without affecting plasma PAI-1 levels in db/db mice, whereas the FD did not affect these parameters in wild-type mice. Importantly, 24 hours of FD increased the plasma PAI-1 content in wild-type (1.9-fold, p < 0.01) but not in db/db mice, although adipose PAI-1 mRNA levels were significantly increased in db/db mice. The plasma PAI-1 content significantly correlated with hepatic PAI-1 mRNA levels in wild-type (r = 0.84, p < 0.01) and in db/db (r = 0.63, p < 0.01) mice. However, plasma PAI-1 did not correlate with adipose PAI-1 expression in db/db mice, although adipose tissue in general is thought to be the principal site of PAI-1 production in obesity. Hepatic PAI-1 expression was closely correlated with serum levels of free fatty acids in wild-type (r = 0.72, p < 0.01), but not in db/db mice. Adipose PAI-1 expression significantly correlated with serum corticosterone levels in both genotypes (wild-type, r = 0.52, p < 0.05; db/db, r = 0.51, p < 0.01), suggesting that adipose PAI-1 expression is up-regulated by fasting-induced glucocorticoids. The present findings suggested that fasting differentially affects fibrinolytic activity in obese and lean subjects and that PAI-1 expression in the liver as well as in adipose tissues comprises an important determinant of increased risk for cardiovascular disease in obesity. SN - 0340-6245 UR - https://www.unboundmedicine.com/medline/citation/17938813/Food_deprivation_induces_adipose_plasminogen_activator_inhibitor_1__PAI_1__expression_without_accumulation_of_plasma_PAI_1_in_genetically_obese_and_diabetic_db/db_mice_ L2 - https://medlineplus.gov/obesity.html DB - PRIME DP - Unbound Medicine ER -