Tags

Type your tag names separated by a space and hit enter

Antecedent short-term central nervous system administration of estrogen and progesterone alters counterregulatory responses to hypoglycemia in conscious male rats.
Am J Physiol Endocrinol Metab. 2007 Dec; 293(6):E1511-6.AJ

Abstract

The aim of this study was to test the hypothesis that antecedent short-term administration of estradiol or progesterone into the central nervous system (CNS) reduces levels of neuroendocrine counterregulatory hormones during subsequent hypoglycemia. Conscious unrestrained male Sprague-Dawley rats were studied during randomized 2-day experiments. Day 1 consisted of an 8-h lateral ventricle infusion of estradiol (1 mug/mul; n = 9), progesterone (1 mug/mul; n = 9), or saline (0.2 mul/min; n = 10). On day 2, a 2-h hyperinsulinemic (30 pmol.kg(-1).min(-1)) hypoglycemic (2.9 +/- 0.2 mM) clamp was performed on all rats. Central administration of estradiol on day 1 resulted in significantly lower plasma epinephrine levels during hypoglycemia compared with saline, whereas central administration of progesterone resulted in increased levels of plasma norepinephrine and decreased levels of corticosterone both at baseline and during hypoglycemia. Glucagon responses during hypoglycemia were unaffected by prior administration of estradiol or progesterone. Endogenous glucose production following day 1 estradiol was significantly lower during day 2 hypoglycemia, and consequently, the glucose infusion rate to maintain the glycemia was significantly greater after estradiol administration compared with saline. These data suggest that 1) CNS administration of both female reproductive hormones can have rapid effects in modulating levels of counterregulatory hormones during subsequent hypoglycemia in conscious male rats, 2) forebrain administration of reproductive hormones can significantly reduce pituitary adrenal and sympathetic nervous system drive during hypoglycemia, 3) reproductive steroid hormones produce differential effects on sympathetic nervous system activity during hypoglycemia, and 4) reduction of epinephrine resulted in significantly blunted metabolic counterregulatory responses during hypoglycemia.

Links

FREE Publisher Full Text

Authors+Show Affiliations

Sandoval DA
Univ. of Cincinnati, 2170 E. Galbraith Rd., Bldg. E., Rm. 317, Cincinnati, OH 45237, USA. Darleen.Sandoval@uc.edu
Gong B
No affiliation info available
Davis SN
No affiliation info available

MeSH

AnimalsBlood GlucoseCentral Nervous SystemCorticosteroneEpinephrineEstradiolGlucagonGlucose Clamp TechniqueHyperinsulinismHypoglycemiaInfusions, ParenteralInsulinMaleNorepinephrineProgesteroneRatsRats, Sprague-Dawley

Pub Type(s)

Journal Article
Research Support, N.I.H., Extramural

Language

eng

PubMed ID

17940215

Citation

Sandoval, Darleen A., et al. "Antecedent Short-term Central Nervous System Administration of Estrogen and Progesterone Alters Counterregulatory Responses to Hypoglycemia in Conscious Male Rats." American Journal of Physiology. Endocrinology and Metabolism, vol. 293, no. 6, 2007, pp. E1511-6.
Sandoval DA, Gong B, Davis SN. Antecedent short-term central nervous system administration of estrogen and progesterone alters counterregulatory responses to hypoglycemia in conscious male rats. Am J Physiol Endocrinol Metab. 2007;293(6):E1511-6.
Sandoval, D. A., Gong, B., & Davis, S. N. (2007). Antecedent short-term central nervous system administration of estrogen and progesterone alters counterregulatory responses to hypoglycemia in conscious male rats. American Journal of Physiology. Endocrinology and Metabolism, 293(6), E1511-6.
Sandoval DA, Gong B, Davis SN. Antecedent Short-term Central Nervous System Administration of Estrogen and Progesterone Alters Counterregulatory Responses to Hypoglycemia in Conscious Male Rats. Am J Physiol Endocrinol Metab. 2007;293(6):E1511-6. PubMed PMID: 17940215.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Antecedent short-term central nervous system administration of estrogen and progesterone alters counterregulatory responses to hypoglycemia in conscious male rats. AU - Sandoval,Darleen A, AU - Gong,Bin, AU - Davis,Stephen N, Y1 - 2007/10/16/ PY - 2007/10/18/pubmed PY - 2008/2/26/medline PY - 2007/10/18/entrez SP - E1511 EP - 6 JF - American journal of physiology. Endocrinology and metabolism JO - Am J Physiol Endocrinol Metab VL - 293 IS - 6 N2 - The aim of this study was to test the hypothesis that antecedent short-term administration of estradiol or progesterone into the central nervous system (CNS) reduces levels of neuroendocrine counterregulatory hormones during subsequent hypoglycemia. Conscious unrestrained male Sprague-Dawley rats were studied during randomized 2-day experiments. Day 1 consisted of an 8-h lateral ventricle infusion of estradiol (1 mug/mul; n = 9), progesterone (1 mug/mul; n = 9), or saline (0.2 mul/min; n = 10). On day 2, a 2-h hyperinsulinemic (30 pmol.kg(-1).min(-1)) hypoglycemic (2.9 +/- 0.2 mM) clamp was performed on all rats. Central administration of estradiol on day 1 resulted in significantly lower plasma epinephrine levels during hypoglycemia compared with saline, whereas central administration of progesterone resulted in increased levels of plasma norepinephrine and decreased levels of corticosterone both at baseline and during hypoglycemia. Glucagon responses during hypoglycemia were unaffected by prior administration of estradiol or progesterone. Endogenous glucose production following day 1 estradiol was significantly lower during day 2 hypoglycemia, and consequently, the glucose infusion rate to maintain the glycemia was significantly greater after estradiol administration compared with saline. These data suggest that 1) CNS administration of both female reproductive hormones can have rapid effects in modulating levels of counterregulatory hormones during subsequent hypoglycemia in conscious male rats, 2) forebrain administration of reproductive hormones can significantly reduce pituitary adrenal and sympathetic nervous system drive during hypoglycemia, 3) reproductive steroid hormones produce differential effects on sympathetic nervous system activity during hypoglycemia, and 4) reduction of epinephrine resulted in significantly blunted metabolic counterregulatory responses during hypoglycemia. SN - 0193-1849 UR - https://www.unboundmedicine.com/medline/citation/17940215/Antecedent_short_term_central_nervous_system_administration_of_estrogen_and_progesterone_alters_counterregulatory_responses_to_hypoglycemia_in_conscious_male_rats_ L2 - https://journals.physiology.org/doi/10.1152/ajpendo.00340.2007?url_ver=Z39.88-2003&rfr_id=ori:rid:crossref.org&rfr_dat=cr_pub=pubmed DB - PRIME DP - Unbound Medicine ER -