Tags

Type your tag names separated by a space and hit enter

Generation of conditional knockout alleles for PDGF-C.
Genesis. 2007 Oct; 45(10):653-7.G

Abstract

PDGF-C is a newly identified member of the platelet-derived growth factor (PDGF) family, which is involved in multiple cellular functions by signaling through PDGF receptor (PDGFR)-alphaalpha and alphabeta dimers. PDGF-C deficiency is perinatal lethal due to the formation of cleft palate. To further characterize the cellular function of PDGF-C during both embryonic and postnatal development, we have generated two conditional alleles of the Pdgf-c gene in which two loxP sites flank exon 5. Global Cre-mediated excision of the floxed exon 5 in these alleles resulted in a complete loss of PDGF-C expression and caused embryonic defects identical to those previously described for the PDGF-C null embryos. These conditional alleles will therefore be the important genetic tools for dissecting the spatial and temporal roles of PDGF-C during development and in adult tissues. Furthermore, from this work, we have also described a simple approach for creating mouse conditional alleles in an efficient manner.

Authors+Show Affiliations

Department of Biochemistry and Medical Genetics, University of Manitoba, Winnipeg, Manitoba, Canada.No affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

17941048

Citation

Wu, Xiaoli, and Hao Ding. "Generation of Conditional Knockout Alleles for PDGF-C." Genesis (New York, N.Y. : 2000), vol. 45, no. 10, 2007, pp. 653-7.
Wu X, Ding H. Generation of conditional knockout alleles for PDGF-C. Genesis. 2007;45(10):653-7.
Wu, X., & Ding, H. (2007). Generation of conditional knockout alleles for PDGF-C. Genesis (New York, N.Y. : 2000), 45(10), 653-7.
Wu X, Ding H. Generation of Conditional Knockout Alleles for PDGF-C. Genesis. 2007;45(10):653-7. PubMed PMID: 17941048.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Generation of conditional knockout alleles for PDGF-C. AU - Wu,Xiaoli, AU - Ding,Hao, PY - 2007/10/18/pubmed PY - 2007/12/14/medline PY - 2007/10/18/entrez SP - 653 EP - 7 JF - Genesis (New York, N.Y. : 2000) JO - Genesis VL - 45 IS - 10 N2 - PDGF-C is a newly identified member of the platelet-derived growth factor (PDGF) family, which is involved in multiple cellular functions by signaling through PDGF receptor (PDGFR)-alphaalpha and alphabeta dimers. PDGF-C deficiency is perinatal lethal due to the formation of cleft palate. To further characterize the cellular function of PDGF-C during both embryonic and postnatal development, we have generated two conditional alleles of the Pdgf-c gene in which two loxP sites flank exon 5. Global Cre-mediated excision of the floxed exon 5 in these alleles resulted in a complete loss of PDGF-C expression and caused embryonic defects identical to those previously described for the PDGF-C null embryos. These conditional alleles will therefore be the important genetic tools for dissecting the spatial and temporal roles of PDGF-C during development and in adult tissues. Furthermore, from this work, we have also described a simple approach for creating mouse conditional alleles in an efficient manner. SN - 1526-954X UR - https://www.unboundmedicine.com/medline/citation/17941048/Generation_of_conditional_knockout_alleles_for_PDGF_C_ L2 - https://doi.org/10.1002/dvg.20339 DB - PRIME DP - Unbound Medicine ER -