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Phex mutation causes overexpression of FGF23 in teeth.
Arch Oral Biol 2008; 53(2):99-104AO

Abstract

OBJECTIVE

Hyp mice have a disorder in phosphate homeostasis, and display hypo-mineralization in bones and teeth, while the Phex (phosphate regulating gene homologies to endopeptidase on the X chromosome) gene in Hyp mice has a deletion of the 3' end. We investigated whether a mutation of Phex has an effect on the expression level of fibroblast growth factor 23 (FGF23), one of the key factors of phosphate homeostasis, in developing teeth of Hyp mice.

DESIGN

RT-PCR and in situ hybridisation analyses for FGF23 were performed using developing teeth of WT mice. Quantitative RT-PCR analyses for FGF23 were performed using the tooth germs of WT and Hyp mice in both in vivo and in vitro experiments.

RESULTS

Undifferentiated and early secretory ameloblasts as well as odontoblasts expressed FGF23 mRNA during early tooth development. Further, quantitative RT-PCR analyses revealed that the amount of FGF23 mRNA in Hyp mouse teeth was significantly higher than that in wild type mice.

CONCLUSIONS

These findings suggest that loss of Phex function is related to overexpression of FGF23 in teeth, which is an intrinsic defect of Hyp mouse teeth.

Authors+Show Affiliations

Department of Pediatric Dentistry, Osaka University Graduate School of Dentistry, 1-8 Yamadaoka, Suita, Osaka 565-0871, Japan.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

17942069

Citation

Onishi, T, et al. "Phex Mutation Causes Overexpression of FGF23 in Teeth." Archives of Oral Biology, vol. 53, no. 2, 2008, pp. 99-104.
Onishi T, Umemura S, Shintani S, et al. Phex mutation causes overexpression of FGF23 in teeth. Arch Oral Biol. 2008;53(2):99-104.
Onishi, T., Umemura, S., Shintani, S., & Ooshima, T. (2008). Phex mutation causes overexpression of FGF23 in teeth. Archives of Oral Biology, 53(2), pp. 99-104.
Onishi T, et al. Phex Mutation Causes Overexpression of FGF23 in Teeth. Arch Oral Biol. 2008;53(2):99-104. PubMed PMID: 17942069.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Phex mutation causes overexpression of FGF23 in teeth. AU - Onishi,T, AU - Umemura,S, AU - Shintani,S, AU - Ooshima,T, Y1 - 2007/10/17/ PY - 2007/06/18/received PY - 2007/08/15/revised PY - 2007/08/18/accepted PY - 2007/10/19/pubmed PY - 2008/5/16/medline PY - 2007/10/19/entrez SP - 99 EP - 104 JF - Archives of oral biology JO - Arch. Oral Biol. VL - 53 IS - 2 N2 - OBJECTIVE: Hyp mice have a disorder in phosphate homeostasis, and display hypo-mineralization in bones and teeth, while the Phex (phosphate regulating gene homologies to endopeptidase on the X chromosome) gene in Hyp mice has a deletion of the 3' end. We investigated whether a mutation of Phex has an effect on the expression level of fibroblast growth factor 23 (FGF23), one of the key factors of phosphate homeostasis, in developing teeth of Hyp mice. DESIGN: RT-PCR and in situ hybridisation analyses for FGF23 were performed using developing teeth of WT mice. Quantitative RT-PCR analyses for FGF23 were performed using the tooth germs of WT and Hyp mice in both in vivo and in vitro experiments. RESULTS: Undifferentiated and early secretory ameloblasts as well as odontoblasts expressed FGF23 mRNA during early tooth development. Further, quantitative RT-PCR analyses revealed that the amount of FGF23 mRNA in Hyp mouse teeth was significantly higher than that in wild type mice. CONCLUSIONS: These findings suggest that loss of Phex function is related to overexpression of FGF23 in teeth, which is an intrinsic defect of Hyp mouse teeth. SN - 0003-9969 UR - https://www.unboundmedicine.com/medline/citation/17942069/Phex_mutation_causes_overexpression_of_FGF23_in_teeth L2 - https://linkinghub.elsevier.com/retrieve/pii/S0003-9969(07)00201-4 DB - PRIME DP - Unbound Medicine ER -