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Lauryl sulphate haemoglobin: a non-hazardous substitute for HiCN in haemoglobinometry.
Clin Lab Haematol. 1991; 13(3):279-90.CL

Abstract

The haemiglobincyanide (HiCN) method for measuring haemoglobin is used extensively worldwide; its advantage is the ready availability of a stable and internationally accepted reference standard/calibrator. However, its use may create a problem, especially in automated analysers, as the waste disposal of large volumes of reagent containing cyanide may constitute a potential toxic hazard. As an alternative, conversion of haemoglobin to a sulphate derivative by nontoxic sodium lauryl sulphate has been proposed and is available as a commercial product from Toa Medical Electronics Co. Ltd., Japan. This evaluation has shown it to be as reliable and reproducible as HiCN for measuring haemoglobin at all concentrations, by both spectrophotometry and automated analyser. Maximum absorbance is at 534 nm. Total conversion occurs almost instantaneously, and includes methaemoglobin but not sulphaemoglobin. HbF is also measured. The only disadvantage is that solutions of haemoglobin-sulphate are stable for only a few hours at room temperature and up to three weeks at 4 degrees C. Thus, for standardization and calibration of instruments it is necessary to use a sample of blood or lysate the haemoglobin value of which has been assigned initially by the HiCN method.

Authors+Show Affiliations

Department of Haematology, Royal Postgraduate Medical School, London.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Journal Article

Language

eng

PubMed ID

1794230

Citation

Lewis, S M., et al. "Lauryl Sulphate Haemoglobin: a Non-hazardous Substitute for HiCN in Haemoglobinometry." Clinical and Laboratory Haematology, vol. 13, no. 3, 1991, pp. 279-90.
Lewis SM, Garvey B, Manning R, et al. Lauryl sulphate haemoglobin: a non-hazardous substitute for HiCN in haemoglobinometry. Clin Lab Haematol. 1991;13(3):279-90.
Lewis, S. M., Garvey, B., Manning, R., Sharp, S. A., & Wardle, J. (1991). Lauryl sulphate haemoglobin: a non-hazardous substitute for HiCN in haemoglobinometry. Clinical and Laboratory Haematology, 13(3), 279-90.
Lewis SM, et al. Lauryl Sulphate Haemoglobin: a Non-hazardous Substitute for HiCN in Haemoglobinometry. Clin Lab Haematol. 1991;13(3):279-90. PubMed PMID: 1794230.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Lauryl sulphate haemoglobin: a non-hazardous substitute for HiCN in haemoglobinometry. AU - Lewis,S M, AU - Garvey,B, AU - Manning,R, AU - Sharp,S A, AU - Wardle,J, PY - 1991/1/1/pubmed PY - 1991/1/1/medline PY - 1991/1/1/entrez SP - 279 EP - 90 JF - Clinical and laboratory haematology JO - Clin Lab Haematol VL - 13 IS - 3 N2 - The haemiglobincyanide (HiCN) method for measuring haemoglobin is used extensively worldwide; its advantage is the ready availability of a stable and internationally accepted reference standard/calibrator. However, its use may create a problem, especially in automated analysers, as the waste disposal of large volumes of reagent containing cyanide may constitute a potential toxic hazard. As an alternative, conversion of haemoglobin to a sulphate derivative by nontoxic sodium lauryl sulphate has been proposed and is available as a commercial product from Toa Medical Electronics Co. Ltd., Japan. This evaluation has shown it to be as reliable and reproducible as HiCN for measuring haemoglobin at all concentrations, by both spectrophotometry and automated analyser. Maximum absorbance is at 534 nm. Total conversion occurs almost instantaneously, and includes methaemoglobin but not sulphaemoglobin. HbF is also measured. The only disadvantage is that solutions of haemoglobin-sulphate are stable for only a few hours at room temperature and up to three weeks at 4 degrees C. Thus, for standardization and calibration of instruments it is necessary to use a sample of blood or lysate the haemoglobin value of which has been assigned initially by the HiCN method. SN - 0141-9854 UR - https://www.unboundmedicine.com/medline/citation/1794230/Lauryl_sulphate_haemoglobin:_a_non_hazardous_substitute_for_HiCN_in_haemoglobinometry_ L2 - https://onlinelibrary.wiley.com/resolve/openurl?genre=article&sid=nlm:pubmed&issn=0141-9854&date=1991&volume=13&issue=3&spage=279 DB - PRIME DP - Unbound Medicine ER -