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[Construction and immunogenicity of attenuated Salmonella typhimurium harbouring stable DNA vaccine against H5 subtype of avian influenza virus].
Wei Sheng Wu Xue Bao. 2007 Aug; 47(4):662-6.WS

Abstract

The hemagglutinin protein (HA) gene of avian influenza virus was amplified by polymerase chain reaction (PCR) from the recombinant plasmid pVAX1-HA, and subcloned into eukaryotic expression vector pmcDNA3.1+. The HA gene was identified by sequencing. The recombinant plasmids were transformed into attenuated Salmonella typhimurium SL7207, and the recombinants were designated as SL7207 (pmcDNA3.1-HA). In vitro and in vivo experiments showed that the plasmid stability of pmcDNA3.1-HA is apparently higher than pcDNA3.1-HA in SL7207. In order to compare the immune response induced by those two recombinant bacteria, BALB/c mice were immunized orally with them at the dosage of 2 x 10(9) CFU respectively. Both SL7207 (pcDNA3.1-HA) and SL7207 (pmcDNA3.1-HA) initiated HA-specific mucosal antibodies in immunized mice. Furthermore, commercial ISA brown chickens were immunized with SL7207 (pcDNA3.1-HA) and SL7207 (pmcDNA3.1-HA) at the dosage of 5 x 10(9) CFU and boosted two weeks later with the same dose. Intestinal mucosal immune responses were observed and their levels were significantly higher than that of negative and positive control. The result of protective immunity showed that the chicken immunized with SL7207 (pmcDNA3.1-HA) had the protective rate of 79.3%, higher than that of SL7207 (pcDNA3.1-HA) with 56.7%. In summary, the DNA vaccine delivered by attenuated Salmonella typhimurium has good immunogenicity. A novel mucosal DNA vaccine was developed and could be useful for controlling the infection and epidemic of avian influenza in the poultry.

Authors+Show Affiliations

Tang LH
Jiangsu Key Laboratory of Zoonosis, Yangzhou University, Yangzhou 225009, China.
Pan ZM
No affiliation info available
Cheng NN
No affiliation info available
Jiao XA
No affiliation info available
Zhang XM
No affiliation info available

MeSH

AnimalsChickensGene ExpressionGenetic EngineeringHemagglutinin Glycoproteins, Influenza VirusImmunizationInfluenza A Virus, H5N1 SubtypeInfluenza VaccinesInfluenza in BirdsMiceMice, Inbred BALB CRandom AllocationSalmonella typhimuriumVaccines, DNA

Pub Type(s)

English Abstract
Journal Article
Research Support, Non-U.S. Gov't

Language

chi

PubMed ID

17944368

Citation

Tang, Li-hua, et al. "[Construction and Immunogenicity of Attenuated Salmonella Typhimurium Harbouring Stable DNA Vaccine Against H5 Subtype of Avian Influenza Virus]." Wei Sheng Wu Xue Bao = Acta Microbiologica Sinica, vol. 47, no. 4, 2007, pp. 662-6.
Tang LH, Pan ZM, Cheng NN, et al. [Construction and immunogenicity of attenuated Salmonella typhimurium harbouring stable DNA vaccine against H5 subtype of avian influenza virus]. Wei Sheng Wu Xue Bao. 2007;47(4):662-6.
Tang, L. H., Pan, Z. M., Cheng, N. N., Jiao, X. A., & Zhang, X. M. (2007). [Construction and immunogenicity of attenuated Salmonella typhimurium harbouring stable DNA vaccine against H5 subtype of avian influenza virus]. Wei Sheng Wu Xue Bao = Acta Microbiologica Sinica, 47(4), 662-6.
Tang LH, et al. [Construction and Immunogenicity of Attenuated Salmonella Typhimurium Harbouring Stable DNA Vaccine Against H5 Subtype of Avian Influenza Virus]. Wei Sheng Wu Xue Bao. 2007;47(4):662-6. PubMed PMID: 17944368.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - [Construction and immunogenicity of attenuated Salmonella typhimurium harbouring stable DNA vaccine against H5 subtype of avian influenza virus]. AU - Tang,Li-hua, AU - Pan,Zhi-ming, AU - Cheng,Ning-ning, AU - Jiao,Xin-an, AU - Zhang,Xiao-ming, PY - 2007/10/20/pubmed PY - 2009/5/6/medline PY - 2007/10/20/entrez SP - 662 EP - 6 JF - Wei sheng wu xue bao = Acta microbiologica Sinica JO - Wei Sheng Wu Xue Bao VL - 47 IS - 4 N2 - The hemagglutinin protein (HA) gene of avian influenza virus was amplified by polymerase chain reaction (PCR) from the recombinant plasmid pVAX1-HA, and subcloned into eukaryotic expression vector pmcDNA3.1+. The HA gene was identified by sequencing. The recombinant plasmids were transformed into attenuated Salmonella typhimurium SL7207, and the recombinants were designated as SL7207 (pmcDNA3.1-HA). In vitro and in vivo experiments showed that the plasmid stability of pmcDNA3.1-HA is apparently higher than pcDNA3.1-HA in SL7207. In order to compare the immune response induced by those two recombinant bacteria, BALB/c mice were immunized orally with them at the dosage of 2 x 10(9) CFU respectively. Both SL7207 (pcDNA3.1-HA) and SL7207 (pmcDNA3.1-HA) initiated HA-specific mucosal antibodies in immunized mice. Furthermore, commercial ISA brown chickens were immunized with SL7207 (pcDNA3.1-HA) and SL7207 (pmcDNA3.1-HA) at the dosage of 5 x 10(9) CFU and boosted two weeks later with the same dose. Intestinal mucosal immune responses were observed and their levels were significantly higher than that of negative and positive control. The result of protective immunity showed that the chicken immunized with SL7207 (pmcDNA3.1-HA) had the protective rate of 79.3%, higher than that of SL7207 (pcDNA3.1-HA) with 56.7%. In summary, the DNA vaccine delivered by attenuated Salmonella typhimurium has good immunogenicity. A novel mucosal DNA vaccine was developed and could be useful for controlling the infection and epidemic of avian influenza in the poultry. SN - 0001-6209 UR - https://www.unboundmedicine.com/medline/citation/17944368/[Construction_and_immunogenicity_of_attenuated_Salmonella_typhimurium_harbouring_stable_DNA_vaccine_against_H5_subtype_of_avian_influenza_virus]_ L2 - https://medlineplus.gov/birdflu.html DB - PRIME DP - Unbound Medicine ER -